Antagonistic effect of bioactive substances on clinically significant antibiotic-resistant and biofilm forming coagulase negative staphylococci

The aim of the submitted project focuses on determination of prevalence of coagulase negative biofilm forming multiresistant staphylococci in samples from environment, potable water, wastewater treatment plants, animals farms and slaughterhouses, milk from dairy cows with contagious mastitis, and clinical samples from human patients from medical services. Another part of the study will be devoted to determination respectively monitoring of the factors of virulence and study of antibacterial and anti-biofilm effect of bioactive compounds of plant (pomiferin) and bacterial (biosurfactants) origin. The results obtained will contribute to the expansion of knowledge in the field of factors and mechanisms controlling and influencing the formation of biofilms and the development of resistance of coagulase-negative staphylococci. Also it can help to develop more effective prophylactic and therapeutic approaches to reduce antibiotic resistance with application in veterinary and human medicine.