Chorioallantoic membrane - in vivo model for study of biocompatibility of materials

Tissue engineering is a combination of cells, biochemical, physico-chemical factors and biomaterials to improve or replace of biological functions. Many definitions of tissue engineering cover applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (cartilage, bone, blood vessels, skin, muscle, nerve, etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning which biomaterials have to guarantee. For the testing of biomaterial, the various methods are used in relation to their biocompatibility with individual organisms. Chicken embryo /or quail embryo is an interesting, recognized a very suitable animal model for studying angiogenesis by ex ovo or in ovo method. This is a low-tech method, which makes it possible to continuously monitor angiogenesis, to easily and quickly obtain results, and to evaluate them in a short time. The project of basic research is focused on the study of angiogenesis of biopolymer composites based on polysaccharides with or/without the hydrogel component implanted on the chick or/quail chorioallantoic membrane using macroscopic, histological, immunohistochemical and molecular methods. Modern and advanced scientific approach of developing new biomaterials used in the present project is characterized by tight multidisciplinary connection of biomaterial engineering methods and in vivo studied systems that with high probability allow assess their suitability as a models for testing biomaterials on angiogenesis.