Stem cell secretome and extracellular vesicles in relation to neuroregeneration and neuroplasticity.

The therapeutic effect of stem cells is often mediated by their paracrine function. Secretome (S) and extracellular vesicles (ECV) (exosomes, microvesicles) released from cells contain an important bioactive molecules (proteins, lipids, miRNA, mRNA, ncRNA, DNA), that can be used to treat severe neurodegenerative diseases. The main goal of our project will be to influence several post-traumatic factors in nerve tissue by using secretome and ECV derived from mesenchymal stem cells (S-MSC, ECV-MSC). The experiments will be performed initially under in in vitro conditions and subsequently in vivo in an animal model. We aim to clarify the mechanisms of action of S-MSC, ECV-MSC to reduce pro-inflammatory cytokines release from microglia/macrophages, reduce glial scar, stimulate neural fiber growth and revascularization in vitro and in vivo. With our research, we will contribute to the development of new therapies in regenerative medicine with the potential for transfer to veterinary and human clinical practice.