Metabolic load and endocrine dysregulation in the reproductive cycle of mares.

The project is aimed at obtain a new knowledge about the dynamics and impact of metabolic and endocrine dysregulations during the reproductive cycles of mares. Pregnancy, lactation, but also the period of estrus and ovulation are physiological but stressful periods, which are characterized by many endocrine changes. Changes in serum concentrations of cortisol, thyroid hormones, glucagon, ß-endorphins, P4, FSH, LH, leptin and adiponectin will be monitored to monitor animal welfare, stress and emotional responses. However, the concentration of hormones can be influenced by several factors, such as nutrition, climate, physiological state of the organism, breed, age and activity. The obtained results will be thoroughly analyzed in response to stressful conditions. USG and X-ray, RIA, ELISA and PCR methods will be used to achieve the set goals.