Molecular characteristics of selected zoonotic species and genotypes of intestinal pathogens in the environment in Slovakia

The project will focus on the innovation of molecular tools for species and genotype analysis of DNA of zoonotic species of intestinal parasites, focusing on cryptosporidiosis, microsporidiosis, and blastocystosis. It builds on the APVV-15-0134 project, deepens and extends the lessons learned from this issue and focuses on the introduction of new molecular method multilocus sequencing MLST, which should genotyping of intestinal parasites. Due to the fact that the host spectrum of these pathogens is rapidly expanding and we confirmed in our findings the presence of zoonotic species also in the environment (water reservoirs), it is necessary to extend the area of interest to water, soil, wastewater and the environment especially in the field of animal breeding. Concrete benefits will be a complex picture of the occurrence and spread of zoonotic species and genotypes of these intestinal parasites on the territory of Slovakia, not only in the hosts, but also in the environment.