ProjectsNew players in nanotherapy of neurodegenerative diseases: conditioned media (KM) and extracellular vesicles (EV) of somatic stem cells

New players in nanotherapy of neurodegenerative diseases: conditioned media (KM) and extracellular vesicles (EV) of somatic stem cells

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Identifier	APVV-19-0193
Funding scheme	Slovak research and development agency
Principal investigator at UVLF	Čížková Daša, prof. MVDr., DrSc.
Project duration	01.07.2020 – 30.05.2024

The therapeutic potential of mesenchymal stem cells (MSC) is often mediated by their paracrine function. The analysis of secretory factors and the possibility of their use in regenerative medicine is currently being studied intensively with the aim of introducing this type of treatment into clinical application. Conditioned media (KM) and exosomes (EV) released by cells contain important bioactive molecules (proteins, lipids, metabolites, miRNA, mRNA, ncRNA, DNA) that have their specific content. Exosomes are able to deliver or transmit bio-information and relevant genetic information and proteins between nearby or distant cells and can subsequently modulate recipient cells. The aim of the project is to determine genomic and proteomic profile of KM and EV isolated from canine MSC of adult and neonatal origin. At the same time, in vitro conditions should verify their biological activity and select those KMs or EVs that potentiate endogenous regeneration and affect the immune response. Part of the last phase of the project will be the study and evaluation of the efficacy of KM and EV-mediated nanotherapy in dogs with selected neurodegenerative diseases (canine cognitive dysfunction, retinal degenerative changes, corneal trauma, spinal cord injury), which may be a suitable may be a suitable substitute for cellular therapy in veterinary practice and an ideal animal model for human medicine.

Project outputs

CAPUZ, Alice - SYLVAIN, Osien - KARNOUB, Melodie Anna - ABOULOUARD, Soulaimane - LAURENT, Estelle - COYAUD, Etienne - RAFFO-ROMERO, Antonella - DUHAMEL, Marie - BONNEFOND, Amélie - DERHOURHI, Mehdi - TREROTOLA, Marco - YAZIDI-BELKOURA, Ikram El - DEVOS, David - ŽILKOVÁ, Monika - KOBIESSY, Firas - VANDEN ABEELE, Fabien - FOURNIER, Isabelle - ČÍŽKOVÁ, Dáša - RODET, Franck - SALZET, Michel. Astrocytes express aberrant immunoglobulins as putative gatekeeper of astrocytes to neuronal progenitor conversion. In: Cell death and disease. 2023. ISSN 2041-4889, Vol. 14, no. 4 (2023), art. no. 237, p. [1-16]. (2023: 8.1 - IF, 8.6 - IF 5y, 2.447 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q, 1.997 - ABDC).
Publication details
HUDÁKOVÁ, Terézia - ŠULEKOVÁ, Monika - TAUCHEN, Jan - ŠEMELÁKOVÁ, Martina - VÁRADY, Matúš - POPELKA, Peter. Bioactive compounds and antioxidant activities of selected types of chilli peppers. In: Czech Journal of Food Sciences. 2023. ISSN 1212-1800, Vol. 41, no. 3 (2023), p. 204-211. (2023: 1.2 - IF, 1.4 - IF 5y, 0.349 - SJR, Q3 - SJR Best Q, Q4 - JCR Best Q, 0.198 - ABDC).
Publication details
CAPUZ, Alice - SYLVAIN, Osien - CARDON, Tristan - KARNOUB, Melodie Anna - ABOULOUARD, Soulaimane - RAFFO-ROMERO, Antonella - DUHAMEL, Marie - ČÍŽKOVÁ, Dáša - TREROTOLA, Marco - DEVOS, David - KOBEISSY, Firas - VAN DEN ABELLE, Fabien - BONNEFOND, Amélie - FOURNIER, Isabelle - RODET, Franck - SALZET, Michel. Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes: a new player in neural proteome. In: Cell death and disease. 2023. ISSN 2041-4889, Vol. 14, no. 8 (2023), art. no. 526, p. [1-23]. (2023: 8.1 - IF, 8.6 - IF 5y, 2.447 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q, 1.997 - ABDC).
Publication details