Toll-like 3 receptor mutations and their influence on cell mediated immune response in relation to susceptibility to selected flavivirus infections in animals

Toll like receptors belong to group of receptors, triggering the defense mechanisms and mutations in the TLRs contribute to influence the susceptibility of individuals. The aim of this project is to analyze the divergence in selected locations of the TLR3 in selected animals and a subsequent comparison of mapped out sections among selected species as well as evaluation of TLR3 mutations in individual animal populations and comparison of selected regions of the TLR3 gene in WNV/TBEV seropositive/seronegative animals respectively. Also this project focuses on evaluation of polyinosine-polycitidylic acid synthetic analog of dsRNA) stimulated cells immune response in association with TLR3 mutation. Benefits in basic research field will be the enrichment of the Genbank and detection of cross-species differences in genes. Knowledge about natural resistance influence the susceptibility of target species and serve as a tool for genetic selection and can serve as a tool for further host-pathogen interaction studies.