Use of a functional 3D model of chicken intestine to study the effect of new lactobacillus isolates during infection by major zoonotic foodborne infections

The world is dominated by the increased interest in the consumption of poultry meat, which is the main source of infection of the most commonly reported foodborne infections in humans. In prevention and therapy, the application of probiotic strains is proving to be a promising choice. In common practice, potential probiotic strains are tested first from a microbiological point of view in vitro and then on cell lines. The most commonly used are 2D cell lines, which are not a perfect model for the in vitro study of the mechanism of interaction between a probiotic bacterium and a host, because they are not able to realistically mimic the natural reactions of a living organism. The use of 3D models of cell lines provides a suitable model for the detailed study of bacterial-gut interactions. The project will focus on testing the probiotic properties of newly isolated lactobacilli 3D working model of the intestine of chickens, which represents a promising opportunity to acquire new knowledge of the immune system.