Detection of DNA damage in lymphocytes after exposure to pesticides
| Identifier | 09/2017 |
|---|---|
| Funding scheme | UVMP Internal grant agency |
| Principal investigator at UVLF | Galdíková Martina, MVDr., PhD. |
| Project duration | 01.05.2017 – 30.04.2018 |
The project will focus on monitoring the extent of damage to bovine lymphocytes by selected neonicotinoid insecticides. The effects of these insecticides on the formation of single- and double-stranded DNA breaks in livestock cells are as yet poorly studied. The extent of damage to the genetic material of cells will be monitored by two techniques: detection of phosphorylated histone H2AX (γH2AX) and comet assay (SCGEsingle cell gel electrophoresis). γH2AX is considered to be a sensitive and quantitative marker of double-strand breaks, which, among other things, may be the result of exposure of cells to long-term chemicals (pesticides). The determination of DNA damage will be complemented by the comet assay, which is increasingly applied in the field of genetic toxicology. The aim of the project is based on current knowledge in human medicine, which suggests that unrepaired (unrepaired) double-strand breaks are a source of genomic instability and have an impact on the subsequent development of cancer.