Etiology and resistance of bacterial pathogens of mastitis in dairy cows and sheep herds situated in marginal parts of Slovakia

The aim of project was to evaluate the prevalence and effectiveness of different antibiotics against mastitis causing microorganisms at the two herds of dairy cows and sheep. The diagnosis of mastitis was performed on the basis of clinical examination of the udder, macroscopic evaluation of milk, determination of somatic cell count (SCC) and bacteriological examination of raw milk samples. Resistance was observed against penicillin (22.2% of Stap. aureus) amoxicillin (22.2% of Stap. aureus and 10.5% of Strep. uberis) and streptomycin (22.2% of Stap. aureus and 52.7% of Strep. uberis). The use of antibiotics in treatment of mastitis and other diseases in dairy ruminants can have a negative impact on public health through the increase in resistant bacteria or bacteria producing resistant genes that pass into the organism of people directly or indirectly.