ProjectsStudy of paracrine signaling of somatic stem cells affected by a targeted stimulatory microenvironment.

Study of paracrine signaling of somatic stem cells affected by a targeted stimulatory microenvironment.

Identifier	1/0122/24
Funding scheme	Scientific grant agency (Ministry of education, science, research and sport of the Slovak Republic)
Principal investigator at UVLF	Čížková Daša, prof. MVDr., DrSc.
Project duration	01.01.2024 – 31.12.2027

A paradigm shift toward paracrine signaling as the primary mechanism of therapeutic efficacy of mesenchymal stem cells (MSCs) is leading to using conditioned medium (CM) in neuroregeneration. KM comprises soluble factors (secretomes) and extracellular vesicles (ECVs) released from MSCs containing functional bioactive molecules. Preconditioning MSCs in a hypoxic or pro-inflammatory microenvironment improves their immunomodulatory, anti-apoptotic, angiogenic and pro-regenerative properties. The goal of the project is to optimize the stimulating hypoxic-proinflammatory intervention of MSCs in vitro conditions in order to create \\\"CM and ECV of a new generation\\\" with higher biological activity, which we will verify on neuronal cell lines and primary cultures. Subsequently, we will perform a combined therapy, administering CM (i.v.) and ECV (locally) with the highest neuroregenerative profile in dogs with spinal cord injury. Stimulated CM and ECV can represent an innovative therapy for veterinary practice.

Project outputs

CAPUZ, Alice - SYLVAIN, Osien - KARNOUB, Melodie Anna - ABOULOUARD, Soulaimane - LAURENT, Estelle - COYAUD, Etienne - RAFFO-ROMERO, Antonella - DUHAMEL, Marie - BONNEFOND, Amélie - DERHOURHI, Mehdi - TREROTOLA, Marco - YAZIDI-BELKOURA, Ikram El - DEVOS, David - ŽILKOVÁ, Monika - KOBIESSY, Firas - VANDEN ABEELE, Fabien - FOURNIER, Isabelle - ČÍŽKOVÁ, Dáša - RODET, Franck - SALZET, Michel. Astrocytes express aberrant immunoglobulins as putative gatekeeper of astrocytes to neuronal progenitor conversion. In: Cell death and disease. 2023. ISSN 2041-4889, Vol. 14, no. 4 (2023), art. no. 237, p. [1-16]. (2023: 8.1 - IF, 8.6 - IF 5y, 2.447 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q, 1.997 - ABDC).
Publication details
HUDÁKOVÁ, Terézia - ŠULEKOVÁ, Monika - TAUCHEN, Jan - ŠEMELÁKOVÁ, Martina - VÁRADY, Matúš - POPELKA, Peter. Bioactive compounds and antioxidant activities of selected types of chilli peppers. In: Czech Journal of Food Sciences. 2023. ISSN 1212-1800, Vol. 41, no. 3 (2023), p. 204-211. (2023: 1.2 - IF, 1.4 - IF 5y, 0.349 - SJR, Q3 - SJR Best Q, Q4 - JCR Best Q, 0.198 - ABDC).
Publication details