Genotoxic and cytotoxic effects of neonicotinoid insecticides
| Identifier | 1/0176/16 |
|---|---|
| Funding scheme | Scientific grant agency (Ministry of education, science, research and sport of the Slovak Republic) |
| Principal investigator at UVLF | Dianovský Ján, prof. MVDr., PhD. |
| Project duration | 01.04.2016 – 31.03.2018 |
Project is focused on the assessment of cytotoxic and genotoxic potential of the selected neonikotinoid insecticides used in agriculture. Induction of bovine lymphocyte damage will be investigated on the DNA level by the methods of comet assay, DNA fragmentation, interactions of chemicals with DNA and changes in expression of some genes included in xenobiotic metabolism and apoptosis. Micronuclei frequencies and detection of unstable chromosomal aberrations will be completed by fluorescent in situ hybridisation using the bovine whole chromosome painting probes, allowing visualisation of stable chromosomal aberrations (translocations). Kinetic measure of enzymes included in xenobiotic metabolism such as superoxide dismutase, glutathione transferase and catalase will be investigated in relation to oxidative stress.