Genetic and epigenetic changes in ruminant cells exposed to pesticides
| Identifier | 1/0242/19 |
|---|---|
| Funding scheme | Scientific grant agency (Ministry of education, science, research and sport of the Slovak Republic) |
| Principal investigator at UVLF | Holečková Beáta, doc. RNDr., PhD. |
| Project duration | 01.01.2019 – 31.12.2021 |
The project is aimed at monitoring the extent of damage to genetic material and the induction of epigenetic changes in
cultured ruminant lymphocytes after application of selected combinations of pesticides. Presence
of double-strand breaks on DNA, the increase of which is associated with disruption of the integrity of the cell genome and tumour
will be assessed by detection of phosphorylated histone H2AX and comet assay. As biomarkers
cytogenetic markers will be used as biomarkers of early genotoxic effect: chromosome aberrations, exchanges
sister chromatids and micronucleus test. The experiments will be complemented by the determination of kinetics and gene
expression of some detoxifying enzymes in relation to oxidative stress and evaluation of the interaction of selected
pesticides with biological macromolecules. At present, little is known about epigenetic changes
induced by pesticides in livestock cells and their impact on health status. Therefore, the project will
also address the identification of DNA methylation changes in some genes for metabolising enzymes.
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