UVLF University portalUniversity of Veterinary Medicine and Pharmacy in Košice
Slovenčina English

Main menu

ProjectsSex-specific microbiome and gene interactions in the pathogenesis of behavioral and gastrointestinal symptoms in the animal model of autism spectrum disorder.

Sex-specific microbiome and gene interactions in the pathogenesis of behavioral and gastrointestinal symptoms in the animal model of autism spectrum disorder.

Back

Identifier20-0114
Funding schemeSlovak research and development agency
Principal investigator at UVLFGancarčíková Soňa, MVDr., PhD.
Name of the project’s principal investigatorAleksandra Tomova doc., MUDr., PHD.
Project duration01.07.2021 – 27.06.2025
Autism spectrum disorder (ASD) is a complex of neurodevelopmental conditions with increasing incidence. Genetic background of ASD explains its development partially, suggesting the contribution of environmental factors, as gut microbiota. Recent research indicates microbiota-gut-brain axis is involved in pathogenesis of neurodevelopmental disorders and modulates many aspects of behavior. Except behavioral, children with ASD frequently have gastrointestinal (GI) manifestations, suggesting the gut microbiota involvement. Changes in the gut microbiota composition in children with ASD are documented and they correlate with behavioral and GI manifestations. Disturbed microbiota impacts intestinal permeability and causes low degree of intestinal inflammation, possible cause of GI complains. Children with ASD have also more frequently the unusual feeding behaviors, as food selectivity. We have found food selectivity was associated with the gut microbiota. On the other side bacterial metabolites are involved in the control of food intake. Other important aspect is that males are more frequently diagnosed with ASD. It has been established, that there is a gender-specificity of gut microbiota, gender-specificity in food intake habits and, finally gender-specificity of behaviour. Thus, increasing evidence supports \\\"three-hit\\\" ASD etiology hypothesis, including genetic load, gender specificity and environmental factor as microbiota, and this project aims to investigate their interconnection in ASD development. Using fecal microbiota transplantation from children with ASD and neurotypical controls of both genders into male and female mice with/without ASD genetic background, we will study the impactof sex-specific gut microbiota and genes on behavioral, GI manifestations and on the character of food intake regulation. Following correction of gut microbiota with probiotics and FMT from neurotypical controls would prove the perspective of this interventions for ASD.

Partners

  • Comenius University Bratislava (Project coordinator)
  • University of Veterinary Medicine and Pharmacy in Košice

Project outputs

  1. KUBAŠOVÁ, Ivana - STROMPFOVÁ, Viola - NEMCOVÁ, Radomíra - GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - HAJDUČKOVÁ, Vanda. Growth of probiotic and pathogenic strains in the presence of different concertation of flaxseed. In: Animal Physiology. Animal Physiology 2022 : Book of abstract: 17th international scientific conference. 1. vyd. Košice : Centrum biovied SAV, 2022. ISBN 978-80-974246-0-2, online, s. 73.

    Publication details

  2. PAČUTA, Ivan - GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - HAJDUČKOVÁ, Vanda - KOLESÁR FECSKEOVÁ, Lívia - MAĎAR, Marián - ŠTRKOLCOVÁ, Gabriela - JANIČKO, Martin - HIJOVÁ, Emília - STROJNÝ, Ladislav - KVAKOVÁ, Monika - KAMLÁROVÁ, Anna - GUĽÁŠOVÁ, Zuzana - AMBRO, Ľuboš - NEMCOVÁ, Radomíra - NEMETOVÁ, Daniela - BERTKOVÁ, Izabela. Quantitative fluorescence in situ hybridization of selected intestinal bacteria of human microbiota-associated murine model. In: Animal Physiology. Animal Physiology 2022 : Book of abstract: 17th international scientific conference. 1. vyd. Košice : Centrum biovied SAV, 2022. ISBN 978-80-974246-0-2, online, s. 84.

    Publication details

  3. HRADICKÁ, Petra - ADAMKOVÁ, Petra - LENHARDT, Ľudovít - GANCARČÍKOVÁ, Soňa - FARKAŠOVÁ IANNACCONE, Silvia - DEMEČKOVÁ, Vlasta. Addressing safety concerns of long-term probiotic use: In vivo evidence from a rat model. In: Journal of Functional Foods. 2023. ISSN 1756-4646, Vol. 104, no. (2023), art. no. 105521, p. [1-11]. (2023: 3.8 - IF, 4.6 - IF 5y, 0.9 - SJR, Q1 - SJR Best Q, Q2 - JCR Best Q, 0.641 - ABDC).

    Publication details

  4. GANCARČÍKOVÁ, Soňa - HAJDUČKOVÁ, Vanda - LAUKO, Stanislav - DEMEČKOVÁ, Vlasta - RYNIKOVÁ, Mária - ADAMKOVÁ, Petra - SASHOVA TOMOVA, Aleksandra - RAŠKOVÁ, Barbara - OSTATNÍKOVÁ, Daniela - NÉMETOVÁ, Daniela - PAČUTA, Ivan. Comparison of gut microbiota of children with autism spectrum disorder and of neurotypical girls. In: Animal Physiology 2023 : 1. vyd. Krakow : Pedagogical University, 2023. online, s. 20.

    Publication details

  5. GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - NÉMETOVÁ, Daniela - HAJDUČKOVÁ, Vanda - SASHOVA TOMOVA, Aleksandra - RAŠKOVÁ, Barbara - OSTATNÍKOVÁ, Daniela - RODÁKOVÁ, Kristína - ŠTEFANCOVÁ, Diana - DEMEČKOVÁ, Vlasta - RYNIKOVÁ, Mária - ADAMKOVÁ, Petra - LECOVÁ, Martina - PAČUTA, Ivan - MUDROŇOVÁ, Dagmar - KOŠČOVÁ, Jana - SOPKOVÁ, Drahomíra. Gastrointestinal alterations in an animal model with autism spectrum disorders. In: Animal Physiology 2023 : 1. vyd. Krakow : Pedagogical University, 2023. online, s. 21.

    Publication details

  6. LAUKO, Stanislav - GANCARČÍKOVÁ, Soňa - HAJDUČKOVÁ, Vanda - HRČKOVÁ, Gabriela - NÉMETOVÁ, Daniela - RODÁKOVÁ, Kristína - ŠTEFANCOVÁ, Diana - LECOVÁ, Martina - PAČUTA, Ivan - DEMEČKOVÁ, Vlasta - RYNIKOVÁ, Mária - ADAMKOVÁ, Petra - SASHOVA TOMOVA, Aleksandra - RAŠKOVÁ, Barbara - OSTATNÍKOVÁ, Daniela - ANDREJČÁKOVÁ, Zuzana - SOPKOVÁ, Drahomíra. Histophatology of intestinal mucosa in PGF animal model with dysbiotic microbiota of children with autism spectrum disorders. In: Animal Physiology 2023 : 1. vyd. Krakow : Pedagogical University, 2023. online, s. 37.

    Publication details

  7. RYNIKOVÁ, Mária - DEMEČKOVÁ, Vlasta - ADAMKOVÁ, Petra - LAUKO, Stanislav - HAJDUČKOVÁ, Vanda - GANCARČÍKOVÁ, Soňa - SASHOVA TOMOVA, Aleksandra - RAŠKOVÁ, Barbara. The promising role of probiotics and prebiotics in managing the altered gut microbiome and gastrointestinal symptoms of autism spectrum disorders. In: Animal Physiology 2023 : 1. vyd. Krakow : Pedagogical University, 2023. online, s. 57-58.

    Publication details

  8. LECOVÁ, Martina - ŠTEFANCOVÁ, Diana - SOPKOVÁ, Drahomíra - RODÁKOVÁ, Kristína - ANDREJČÁKOVÁ, Zuzana - GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - HAJDUČKOVÁ, Vanda - SASHOVA TOMOVA, Aleksandra - VLČKOVÁ, Radoslava. Direct effect of flaxseed components on the hormone production by mice ovaries. In: Animal Physiology 2023 : 1. vyd. Krakow : Pedagogical University, 2023. online, s. 38.

    Publication details

  9. RODÁKOVÁ, Kristína - SOPKOVÁ, Drahomíra - ANDREJČÁKOVÁ, Zuzana - VLČKOVÁ, Radoslava - GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - HAJDUČKOVÁ, Vanda - PETRILLA, Vladimír - LECOVÁ, Martina - ŠTEFANCOVÁ, Diana - NÉMETOVÁ, Daniela. Therapeutic potential of FMT on the biochemical marker of lactate dehydrogenase (LDH) and its isoenzymes in gastrointestinal disorders using the PGF animal model. In: Animal Physiology 2023 : 1. vyd. Krakow : Pedagogical University, 2023. online, s. 56.

    Publication details

  10. RYNIKOVÁ, Mária - ADAMKOVÁ, Petra - GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - HAJDUČKOVÁ, Vanda - RAŠKOVÁ, Barbara - SASHOVA TOMOVA, Aleksandra - DEMEČKOVÁ, Vlasta. Synbiotic improves gut microbiome diversity and gastrointestinal symptoms in preclinical study of autism spectrum disorder. In: Gut Microbiota for Health : 1. vyd. Vienna : ESNM, 2023. online, s. 1-2 (No 24).

    Publication details

  11. NÉMETOVÁ, Daniela - GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - HRČKOVÁ, Gabriela - HAJDUČKOVÁ, Vanda - PAČUTA, Ivan - SASHOVA TOMOVA, Aleksandra - RAŠKOVÁ, Barbara - OSTATNÍKOVÁ, Daniela. Morfologické a funkčné zmeny gastrointestinálneho traktu asociované s poruchami autistického spektra u pseudo germ-free (PGF) animálneho modelu. In: Vedecké práce doktorandov 2023 : Zborník zo seminára doktorandov venovaného pamiatke akademika Boďu : XVIII. ročník. 1. vyd. Košice : ÚFHZ SAV, 2023. ISBN 978-80-974246-4-0, s. 58-60.

    Publication details

  12. RYNIKOVÁ, Mária - ADAMKOVÁ, Petra - GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - HAJDUČKOVÁ, Vanda - RAŠKOVÁ, Barbara - SASHOVA TOMOVA, Aleksandra - DEMEČKOVÁ, Vlasta. Využitie synbiotík na zmiernenie črevnej dysbiózy a gastrointestinálnych príznakov porúch autistického spektra. In: Vedecké práce doktorandov 2023 : Zborník zo seminára doktorandov venovaného pamiatke akademika Boďu : XVIII. ročník. 1. vyd. Košice : ÚFHZ SAV, 2023. ISBN 978-80-974246-4-0, s. 73-75.

    Publication details

  13. NÉMETOVÁ, Daniela - GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - HRČKOVÁ, Gabriela - HAJDUČKOVÁ, Vanda - DEMEČKOVÁ, Vlasta - RYNIKOVÁ, Mária - RODÁKOVÁ, Kristína - ŠTEFANCOVÁ, Diana - LECOVÁ, Martina - SASHOVA TOMOVA, Aleksandra - RAŠKOVÁ, Barbara - OSTATNÍKOVÁ, Daniela - PAČUTA, Ivan - STROMPFOVÁ, Viola - KUBAŠOVÁ, Ivana - SOPKOVÁ, Drahomíra. Morfologické a funkčné zmeny gastrointestinálneho traktu asociované s poruchami autistického spektra u pseudo germ-free (PGF) animálneho modelu. In: Mikro – Imuno Seminár Doktorandov 2024 : Zborník vedeckých prác. 1. vyd. Košice : UVLF, 2024. ISBN 978-80-8077-820-0, s. 27-31.

    Publication details

  14. BABJÁKOVÁ, Diana - LECOVÁ, Martina - RODÁKOVÁ, Kristína - GANCARČÍKOVÁ, Soňa - SOPKOVÁ, Drahomíra - VLČKOVÁ, Radoslava. Vplyv metabolitov ľanového semena na proliferáciu a apoptózu buniek endometria myší [The effect of flaxseed metabolites on proliferation and apoptosis in the mouse endometrium]. In: 27. Košický morfologický deň: Miesto morfológie v súčasnej medicíne : Zborník vedeckých prác. 1. vyd. Košice : UPJŠ v Košiciach, 2024. ISBN 978-80-574-0313-5, online, s. 17-22.

    Publication details

  15. BABJÁKOVÁ, Diana - LECOVÁ, Martina - RODÁKOVÁ, Kristína - GANCARČÍKOVÁ, Soňa - SOPKOVÁ, Drahomíra - VLČKOVÁ, Radoslava. The effect of enterolactone and n-3 PUFAs on the proliferation and apoptosis in the mouse endometrium. In: Animal Physiology 2024. Animal Physiology 2024 : Book of abstracts from 19th International Scientific Conference. 1. vyd. Nitra : SPU v Nitre, 2024. ISBN 978-80-552-2744-3, online, s. 12.

    Publication details

  16. ŠUHAJDOVÁ, Gabriela. Vplyv fekálnej transplantácie mikrobiómu na aktivitu tráviacich enzýmov u myší : diplomová práca. Košice : UVLF 2025. 58 l + CD-ROM.

    Publication details

  17. RODÁKOVÁ, Kristína - ANDREJČÁKOVÁ, Zuzana - VLČKOVÁ, Radoslava - GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - SPIŠÁKOVÁ, Daniela - KOZIOŁ, Katarzyna - DEMEČKOVÁ, Vlasta - KOZIOROWSKA, Anna - RYNIKOVÁ, Mária - STROMPFOVÁ, Viola - SASHOVA TOMOVA, Aleksandra - OSTATNÍKOVÁ, Daniela - SOPKOVÁ, Drahomíra. Pancreatic lipase secretion in the pancreas and its production in duodenal enterocytes following fecal microbiota transplantation, probiotics, and prebiotic administration in a pseudo germ-free animal model. In: Morphology 2025 : Olomouc : UNIVERZITA PALACKÉHO, 2025. ISBN 978-80-244-6680-4, s. 64-65.

    Publication details

  18. GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - SPIŠÁKOVÁ, Daniela - DEMEČKOVÁ, Vlasta - RYNIKOVÁ, Mária - ŠMAJDA RODÁKOVÁ, Kristína - STROMPFOVÁ, Viola - HRČKOVÁ, Gabriela - FARKAŠOVÁ, Soňa - RAŠKOVÁ, Barbara - OSTATNÍKOVÁ, Daniela - SASHOVA TOMOVA, Aleksandra. Effects of probiotic and prebiotic modulation of gut microbiota on gastrointestinal symptoms associated with autism spectrum disorders. In: Targeting Microbiota : Padova : International Society of Microbiota, 2025. s. 75.

    Publication details

  19. SUŠIENKOVÁ, Petronela - KORITKOVÁ, Dominika Monika - DUDOVÁ, Natália - BORBÉLYOVÁ, Diana - GANCARČÍKOVÁ, Soňa - LAUKO, Stanislav - RODÁKOVÁ, Kristína - SPIŠÁKOVÁ, Daniela - SASHOVA TOMOVA, Aleksandra. Vplyv transplantácie fekálneho mikrobiómu na správanie podobné poruchám autistického spektra u samíc pseudo germ-free myší. In: 100. Fyziologické dny : Praha : Fyziologický ústav 1. lékařské fakulty Univerzity Karlovy, 2025. s. 51-52.

    Publication details

  20. ŠMAJDA RODÁKOVÁ, Kristína - GANCARČÍKOVÁ, Soňa - DEMEČKOVÁ, Vlasta - LAUKO, Stanislav - RYNÍKOVÁ, Mária - ANDREJČÁKOVÁ, Zuzana - SPIŠÁKOVÁ, Daniela - FUSEK, Michal - VLČKOVÁ, Radoslava - STROMPFOVÁ, Viola - TOMOVÁ, Alexandra - RAŠKOVÁ, Barbara - SOPKOVÁ, Drahomíra. Modulation of gut–liver axis by ASD-associated microbiota and synbiotic intervention in a pseudo-germ-free mouse model. In: Applied Sciences. 2026. ISSN 2076-3417, Vol. 16, no. 11 (2026), art. no. 5529, p. [1-35]. (2024: 2.5 - IF, 162 - H-index, 2.7 - IF 5y, 0.521 - SJR, Q2 - SJR Best Q, Q2 - JCR Best Q, 0.438 - ABDC).

    Publication details