The complex solution of molecular diagnostics in choosen opportunistic infections and standardization of individual methods

The global spread of opportunistic infections as it is evident from the increased frequency of infections in human and animal populations refers to the requirement of quick, sensitive and exact diagnostic methods. The most sensitive proof on the level of “corpus delicti” is the direct isolation of microorganism (bacteria, virus, protozoa, fungi, etc.) from the analyzed material that was obtained from a patient. We can observe it especially during the current diagnostics of opportunistic infections that become important due to the more frequent occurrence of immunosupressors of different etiology. The diagnostics of these infections are not limited only for human population (AIDS patients, patients after transplantations and cancer patients) but many immunosupressed factors also occur in animal population. These factors make possible a clinical manifestation of the opportunistic infection. Their diagnostics is currently neglected and therefore it is important to standardize methods for quick and precise d