Development of protocols for the molecular biological study of pathogenicity and virulence factors of medically important bacterial pathogens

The project will focus on the development of protocols focused on the currently used molecular biology methods, used to study the pathogenicity and virulence factors of medically important bacterial pathogens, primarily the agents of zoonoses (diseases transmissible from animals to humans). The preferred method of studying the pathogenicity and virulence factors of bacterial pathogens involves working with living organisms in natural - in vivo or in laboratory - in vitro conditions. Knowledge of the complete nucleotide sequence of the genomes of almost all medically important bacterial pathogens allows the research of pathogenicity and virulence factors to be advanced to the level of in silico analysis (i.e. based on works or computer simulations) using bioinformatics methods. New knowledge about the pathogenicity and virulence factors of medically important bacterial pathogens and, above all, agents with zoonotic potential, reveal a detailed view of the mechanism of pathogenesis of the given disease, which provides innovative possibilities for more accurate and faster diagnosis, more effective prevention and therapy. After the publication of the monograph „Bioinformatics and molecular biological tools in the study of pathogenicity and virulence factors of medically important bacterial pathogens“ by the authors of the presented project and the organization of a workshop with high participation and positive feedback not only after its completion, but also during the discussion on individual topics, a request for the publication of protocols reflecting current needs in the field of molecular methods. Doctoral students and researchers are also interested in organizing another workshop aimed at expanding knowledge about current and modern approaches to monitoring pathogenicity factors at the level of gene and protein level. Our goal is to provide the scientific public (researchers and university workers, doctoral students and students) with protocols providing instructions and procedures for working with online databases, analysis of obtained data and their interpretation in the light of biological-medical contexts. In addition to instructions for bioinformatics approaches in molecular biology, the protocols will also contain the currently most used genomic and proteomic procedures. This purpose will be achieved through: protocols for the molecular biological study of pathogenicity and virulence factors of medically important bacterial pathogens. 1. Publications of protocols entitled: Protocols for the molecular biological study of pathogenicity and virulence factors of medically important bacterial pathogens. 2. Organizing the workshop. 3. Expansion of online technical support for working with databases.