ADC Detection of West Nile virus and tick-borne encephalitis virus in birds in Slovakia, using a universal primer set / Csank Tomáš, Bhide Katarína, Bencúrová Elena, Dolinská Saskia, Drzewnioková Petra, Major Peter, Korytár Ľuboš, Bocková Eva, Bhide Mangesh Ramesh, Pistl Juraj, 2016. In: Archives of virology. - ISSN 0304-8608. - Vol. 161, č. 6(2016), s. 1679-1683.

Basic data
OCA1. Surname awarded to the assessed person	Korytár
OCA2. Name awarded to the assessed person	Ľuboš
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/20446
OCA5. Area of assessment	study programme animal welfare and protection · II. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2016
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	UVL.Košice.0024258
OCA9. Hyperlink to the record in CRPA or CRAA	http://www.crepc.sk/portal?fn=*recview&uid=1957240&pageId=resultform&full=0
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000376405500028
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADC Detection of West Nile virus and tick-borne encephalitis virus in birds in Slovakia, using a universal primer set / Csank Tomáš, Bhide Katarína, Bencúrová Elena, Dolinská Saskia, Drzewnioková Petra, Major Peter, Korytár Ľuboš, Bocková Eva, Bhide Mangesh Ramesh, Pistl Juraj, 2016. In: Archives of virology. - ISSN 0304-8608. - Vol. 161, č. 6(2016), s. 1679-1683.
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://link.springer.com/article/10.1007/s00705-016-2828-5
OCA14. Characteristics of the author's contribution	The author participated in conducting the experiments and evaluating the results obtained.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	Detection of TBEVs in the brain of common kite demonstrates the utility of PanFlavi primers in combination with sequencing for flavivirus monitoring. TBEV is endemic in Slovakia and the virus has been detected in birds. Recently, Mikryukova et al. confirmed the involvement of wild birds in natural outbreaks of TBEV in the Tomsk region of Russia. Antigen or viral RNA was found in almost 70% of the bird species tested. However, the highest prevalence was found in the field sparrowhawk, common robin and chickadees. This may be explained by frequent contact with infected ticks rather than susceptibility to infection. Rodents are the main maintainers and reservoir hosts of TBEV. They are abundant in outbreaks and are easily infected by large numbers of Ixodes ricinus ticks. We found TBEV RNA in the brain of a predator, suggesting possible spread of the virus to predators.
Other data
OCA16. Annotation of the output in English	West Nile virus (WNV) is a mosquito-borne neurotropic pathogen that presents a major public health concern. Information on WNV prevalence and circulation in Slovakia is insufficient. Oral and cloacal swabs and bird brain samples were tested for flavivirus RNA by RT-PCR using newly designed generic primers. The species designation was confirmed by sequencing. WNV was detected in swab and brain samples, whereas one brain sample was positive for tick-borne encephalitis virus (TBEV). The WNV sequences clustered with lineages 1 and 2. These results confirm the circulation of WNV in birds in Slovakia and emphasize the risk of infection of humans and horses.
OCA17. List of maximum 5 most significant citations corresponding to the output	Stanko, M., Derdáková, M., Špitalská, E., Kazimírová, M. Ticks and their epidemiological role in Slovakia: from the past till present (2022) Biologia, 77 (6), pp. 1575-1610. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115124568&doi=10.1007%2fs11756-021-00845-3&partnerID=40&md5=408cf89a1f7f05a4d71cd513403ab606 Takahashi, Y., Kobayashi, S., Nakao, R., Kariwa, H., Yoshii, K. Characterization of tick-borne encephalitis virus isolated from tick infesting dog in central Hokkaido in 2018 (2022) Ticks and Tick-borne Diseases, 13 (2), art. no. 101900, . https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123000036&doi=10.1016%2fj.ttbdis.2022.101900&partnerID=40&md5=9c8d108124b1e3c012832fc99c8faa78 Feyer, S., Bartenschlager, F., Bertram, C.A., Ziegler, U., Fast, C., Klopfleisch, R., Müller, K. Clinical, pathological and virological aspects of fatal West Nile virus infections in ten free-ranging goshawks (Accipiter gentilis) in Germany (2021) Transboundary and Emerging Diseases, 68 (2), pp. 907-919. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089397413&doi=10.1111%2ftbed.13759&partnerID=40&md5=ea036f736d625ecc04632bfa049a2d72 Hubálek, Z., Tomešek, M., Kosina, M., Šikutová, S., Straková, P., Rudolf, I. West Nile virus outbreak in captive and wild raptors, Czech Republic, 2018 (2019) Zoonoses and Public Health, 66 (8), pp. 978-981. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070064868&doi=10.1111%2fzph.12638&partnerID=40&md5=5e8c3317667c77793850d8e7b85f20d4 Hubálek, Z., Kosina, M., Rudolf, I., Mendel, J., Straková, P., Tomešek, M. Mortality of Goshawks (Accipiter gentilis) due to west nile virus lineage 2 (2018) Vector-Borne and Zoonotic Diseases, 18 (11), pp. 624-627. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85055836134&doi=10.1089%2fvbz.2018.2289&partnerID=40&md5=154f506533ffe8a6b7d0e72f1fc1f397
OCA18. Characteristics of the output's impact on socio-economic practice	Simultaneous detection of WNV and TBEV by RT-PCR using generic PanFlavi primers targeting the NS5 gene followed by sequencing is useful for monitoring flaviviruses in birds; However, attempts should be made to increase the sensitivity of the test and compare its specificity with other PCR methods) The high proportion of lineage 2 variants in wild birds indicates northward spread and provides a more accurate view of WNV epidemiology) The presence of WNV RNA in the brains of two hawks that died after showing neurological symptoms is the first evidence of West Nile fever in birds of prey in Slovakia.
OCA19. Characteristics of the output and related activities' impact on the educational process	The publication is a suitable teaching aid for students on the Animal Welfare and Conservation degree programme.