ADF Probiotic properties of vaginal lactic acid bacteria selected for harmonization of microenvironment of reproductive apparatus / Styková Eva, Valocký Igor, Novotný František, Guba Peter, 2012. In: Journal of Microbiology, Biotechnology and Food Sciences. - ISSN 1338-5178.

ADF Probiotic properties of vaginal lactic acid bacteria selected for harmonization of microenvironment of reproductive apparatus / Styková Eva, Valocký Igor, Novotný František, Guba Peter, 2012. In: Journal of Microbiology, Biotechnology and Food Sciences. - ISSN 1338-5178. - Vol. 2, č. 1(2012), s. 395-405

Basic data
OCA1. Surname awarded to the assessed person	Valocký
OCA2. Name awarded to the assessed person	Igor
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6025
OCA5. Area of assessment	study programme general veterinary medicine · I. + II. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2012
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	UVL.Košice.0016666
OCA9. Hyperlink to the record in CRPA or CRAA	http://www.crepc.sk/portal?fn=*recview&uid=740024&pageId=resultform&full=0
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000458065700033
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADF Probiotic properties of vaginal lactic acid bacteria selected for harmonization of microenvironment of reproductive apparatus / Styková Eva, Valocký Igor, Novotný František, Guba Peter, 2012. In: Journal of Microbiology, Biotechnology and Food Sciences. - ISSN 1338-5178. - Vol. 2, č. 1(2012), s. 395-405
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.jmbfs.org/wp-content/uploads/2012/08/Stykova_jmbfs_RF.pdf
OCA14. Characteristics of the author's contribution	Participation in the experimental part, evaluation of the achieved results and participation in the final editing of the publication.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The aim of this study was to isolate and screen for probiotic properties of Lactobacillus strains isolated from the vagina of heifers and cows with healthy reproductive systems. The properties initially tested were low pH tolerance, growth at different temperatures, autoaggregation, rapid growth and acid production. The strains were further tested for adherence to vaginal mucus. Negative selection was used and strains that did not meet the test criteria were excluded. Six strains of different taxa were selected from 244 samples collected from 122 heifers and cows. The strains L. buchneri 5/K, L. buchneri 24S8, L. mucosae 29S8, L. mucosae 9/K and L. mucosae BiocenolTM 7697 were selected and identified. These strains showed different biochemical properties even within the taxon and showed differences in the quantification of the selection criteria. Further evaluation of the properties of the selected strains will be carried out with the aim of considering their inclusion in a probiotic for topical use in the reproductive system.
Other data
OCA16. Annotation of the output in English	The aim of this study was isolation and screening of probiotic properties of Lactobacillus strains isolated from the vagina of heifers and cows with healthy reproductive apparatus. Initially tested properties were low pH tolerance, growth at different temperature, autoaggregation, fast growth and acid production. Strains were further tested for adherence to vaginal mucus. Negative selection was used and strains that did not meet tested criterion were excluded. From 244 samples taken from 122 heifers and cows were selected six strains of different taxa. Selected and identified were strains L. buchneri 5/K, L. buchneri 24S8, L. mucosae 29S8, L. mucosae 9/K and L. mucosae BiocenolTM 7697. These strains showed different biochemical properties also within a taxon and exhibited differences in the quantification of selection criteria. Further evaluation of the selected strain properties will be performed to consider their inclusion in a probiotic for local use in reproductive apparatus.
OCA17. List of maximum 5 most significant citations corresponding to the output	DASARI, Subramanyam - SHOURI, Raju Naidu Devanaboyaina - WUDAYAGIRI, Rajendra - VALLURU, Lokanatha. Antimicrobial activity of Lactobacillus against microbial flora of cervicovaginal infections. In Asian Pacific Journal of Tropical Disease. ISSN 22221808, 2014-02-01, 4, 1, pp. 18-24., Registrované v: Scopus AHN, Byeong Ki - MIN, Kyung Cheol - CHO, Sang Hyun - LEE, Dong Geun - KIM, Andre - LEE, Sang Hyeon. Isolation of lactic acid bacteria with anti-MRSA bacteriocin activity and characterization of the bacteriocin product. In Microbiology and Biotechnology Letters. ISSN 1598642X, 2021-06-28, 49, 2, pp. 131-137. Dostupné na: https://doi.org/10.48022/mbl.2012.12008., Registrované v: Scopus SRINIVASAN, M. - ADNANE, M. - ARCHUNAN, G. Significance of cervico-vaginal microbes in bovine reproduction and pheromone production – A hypothetical review. In Research in Veterinary Science, 2021-03-01, 135, pp. 66-71. ISSN 00345288. Dostupné na: https://doi.org/10.1016/j.rvsc.2021.01.003., Registrované v: SCOPUS VADIVOO, V. S. - DHINAKAR RAJ, G. - RANGASAMY, S. - BALASUBRAMANIAN, S. Spectrum of vaginal lactic acid bacteria in indigenous and cross bred cows of India. In Indian Journal of Traditional Knowledge, 2023-10-01, 22, 4, pp. 791-797. ISSN 09725938. Dostupné na: https://doi.org/10.56042/ijtk.v22i4.7203., Registrované v: SCOPUS ZANGIROLAMO, Amanda Fonseca - SOUZA, Anne Kemmer - YOKOMIZO, Deborah Nakayama - MIGUEL, Ana Karolyne Alves - COSTA, Márcio Carvalho da - ALFIERI, Amauri Alcindo - SENEDA, Marcelo Marcondes. Updates and Current Challenges in Reproductive Microbiome: A Comparative Analysis between Cows and Women. In Animals, 2024-07-01, 14, 13, pp. Dostupné na: https://doi.org/10.3390/ani14131971., Registrované v: SCOPUS
OCA18. Characteristics of the output's impact on socio-economic practice	For further testing, strains were selected based on colony morphology, non-hemolytic, Gram-positive, catalase-negative, growing anaerobically, growing in MRS broth adjusted to pH 3.6, growing at 15°C or 45°C, aggregating in MRS broth, with rapid growth and acid production. In the preliminary selection, 20 strains were selected from 244. These strains were tested for antibiotic susceptibility - resistant strains were excluded (unpublished data), for inhibition of pathogenic strains - strains that did not inhibit any tested pathogenic microorganism were excluded (unpublished data), for H2O2 production - strains that did not produce H2O2 (unpublished data), for adherence to vaginal mucus - non-adherent strains were excluded. Negative selection was used and strains that did not meet the test criteria were excluded. According to these criteria, six strains of different taxa were selected. Further evaluation of the properties of the selected strains will be carried out with the aim of considering their inclusion in a probiotic for the prevention and treatment of infectious metritis.
OCA19. Characteristics of the output and related activities' impact on the educational process	The publication is suitable study literature for students of the general veterinary medicine study program.