ADC Paulik, S; Mojzisova, J ; Bajova, V ; Baranova, D; Paulikova, I. Evaluation of canine lymphocyte blastogenesis prior and after in vitro suppression by dog demodicosis serum using ethidium bromide fluorescence assay. In VETERINARNI MEDICINA, 1996, Volume41, Issue1, Page7-12
| Basic data | |
|---|---|
| OCA1. Surname awarded to the assessed person | Mojžišová Vaščinec |
| OCA2. Name awarded to the assessed person | Jana |
| OCA3. Degrees awarded to the assessed person | Dr. h. c. Prof. , DVM, PhD |
| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6013 |
| OCA5. Area of assessment | study programme infectious diseases of animals · III. degree |
| OCA6. Category of the research/artistic/other output | scientific output |
| OCA7. Year of publication of the research/artistic/other output | 1996 |
| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | |
| OCA9. Hyperlink to the record in CRPA or CRAA | |
| Characteristics of the output that is not registered in CRPA or CRAA | |
| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://www.webofscience.com/wos/woscc/full-record/WOS:A1996TW31700002 |
| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | ADC Paulik, S; Mojzisova, J ; Bajova, V ; Baranova, D; Paulikova, I. Evaluation of canine lymphocyte blastogenesis prior and after in vitro suppression by dog demodicosis serum using ethidium bromide fluorescence assay. In VETERINARNI MEDICINA, 1996, Volume41, Issue1, Page7-12 |
| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://pubmed.ncbi.nlm.nih.gov/8619280/ |
| OCA14. Characteristics of the author's contribution | The author had a key role in the experimental part and an important role in the finalization of the publication. |
| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | The mitogen induced blastogenic response of lymphocytes from normal dogs and dogs with generalized demodicosis (GD) was measured by the ethidium bromide (EB) fluorescence assay. Serum from GD dogs significantly suppressed the in vitro reactivity to Con A of peripheral blood lymphocytes (PBL) from normal dogs and GD dogs, however with a different percentage of suppression 40.6 and 81.2%, respectively. As a result, the degree of lymphocyte blastogenesis suppression in GD dogs did not parallel the immunosuppressive potency of their serum. The data indicate that PBL obtained from GD dogs did not respond to Con A as well in the presence of serum from normal dogs as did PBL from normal dogs. IOur results indicated that EB fluorescence assay is a useful method for detection a respondence of canine lymphocyte blastogenesis. |
| Other data | |
| OCA16. Annotation of the output in English | The mitogen induced blastogenic response of lymphocytes from normal dogs and dogs with generalized demodicosis (GD) was measured by the ethidium bromide (EB) fluorescence assay. As a result, the degree of lymphocyte blastogenesis suppression in GD dogs did not parallel the immunosuppressive potency of their serum. The data indicate that PBL obtained from GD dogs did not respond to Con A as well in the presence of serum from normal dogs as did PBL from normal dogs. The basis for this cellular modified response is unknown. It does not appear that the age or the chronicity of the disease are related to this observation. Further studies are necessary to elucidate this relation. The GD dogs showed not only a significant depression of the lymphocyte response to Con A but also enhancement of the ability of unstimulated cells to proliferate was also observed. No significant difference of PBL responsiveness to Con A between healthy dogs with respect to the age and the time of examination was observed. Results indicated that EB fluorescence assay is a useful method for detection a respondence of canine lymphocyte blastogenesis. |
| OCA17. List of maximum 5 most significant citations corresponding to the output | Mueller, R.S., Rosenkrantz, W., Bensignor, E., Karaś-Tęcza, J., Paterson, T., Shipstone, M.A. Diagnosis and treatment of demodicosis in dogs and cats: Clinical consensus guidelines of the World Association for Veterinary Dermatology (2020) Veterinary Dermatology, 31 (1), pp. 5-27 Oliveira, C.D., Larsson, C.E., de Camargo, M.M. Longitudinal assessment of T-lymphocyte subpopulations during generalized demodicosis in dogs and their relationship with remission(2015) Veterinary Dermatology, 26 (1), pp. 18-e6 Kuznetsova, E., Bettenay, S., Nikolaeva, L., Majzoub, M., Mueller, R. Influence of systemic antibiotics on the treatment of dogs with generalized demodicosis(2012) Veterinary Parasitology, 188 (1-2), pp. 148-155 Faldyna, M., Laznicka, A., Toman, M. Immunosuppression in bitches with pyometra(2001) Journal of Small Animal Practice, 42 (1), pp. 5-10 . Toman, M., Svoboda, M., Rybníček, J., Krejčí, J., Faldyna, M., Bárta, O. Immunosuppression in dogs with pyoderma and/or demodicosis (1997) Veterinarni Medicina, 42 (10), pp. 299-306 |
| OCA18. Characteristics of the output's impact on socio-economic practice | The publication brings new theoretical knowledge, thereby enriching social practice. After the introduction of procedures obtained during research into practice, there is also an assumption of an economic benefit. |
| OCA19. Characteristics of the output and related activities' impact on the educational process | The publication enriches students of veterinary medicine with new theoretical knowledge, thereby expanding their educational dimension and preparation for practice. |