ADC A serosurvey of flavivirus infection in horses and birds in Slovakia. Csank T., Korytár Ľ., Major P., Gyuranecz M., Pistl J., Bakonyi T., Drzewnioková P. In Vector-Borne and Zoonotic Diseases, 2018, Roč. 18, č. 4, s. 206-213

Basic data
OCA1. Surname awarded to the assessed person	Korytár
OCA2. Name awarded to the assessed person	Ľuboš
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/20446
OCA5. Area of assessment	study programme infectious diseases of animals · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2018
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	65408
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=0DEA395DAA35342961B2725F
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000425057900001
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADC A serosurvey of flavivirus infection in horses and birds in Slovakia. Csank T., Korytár Ľ., Major P., Gyuranecz M., Pistl J., Bakonyi T., Drzewnioková P. In Vector-Borne and Zoonotic Diseases, 2018, Roč. 18, č. 4, s. 206-213
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.liebertpub.com/doi/10.1089/vbz.2017.2216
OCA14. Characteristics of the author's contribution	Trapping of birds in the field, collection and processing of biological samples, analysis of results, preparation and revision of the manuscript.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	At least three flaviviruses circulate between vectors and vertebrate hosts in central Europe. West Nile virus (WNV) and Usutu virus (USUV) are mosquito-borne viruses that are maintained in nature through an enzootic cycle between mosquitoes and birds. Tick-borne encephalitis virus (TBEV) is a flavivirus that causes cases of human disease in Slovakia every year. The aim of this study was to assess the prevalence of flavivirus infections in horses (n = 145) and birds (n = 109) using ELISA and confirmation by virus neutralization test (VNT). Antibodies to WNV were detected in 11.7% of horses and 11.9% of birds tested and confirmed in 6.9% of horse samples and 9.2% of bird samples. None of the seropositive or dubious horses had WNV IgM (ELISA) and none of the horses tested had USUV neutralizing antibodies. Autochthonous WNV infections were confirmed in 16.7% of horses with no travel history. Most of them were from western Slovakia with known endemic WNV transmission. An autochthonous WNV infection was found in a horse from the Kremnica Hills (central Slovakia) with no data on WNV circulation in the locality and no travel history. TBEV antibodies were detected in 6.2% of horses and confirmed in 3.4%. In two horses, it was not possible to distinguish between WNV and TBEV infection. The presence of specific antibodies to WNV was confirmed in eight birds of prey with non-specific symptoms, one white stork and one house sparrow. The sparrow was captured in a locality in eastern Slovakia where WNV RNA had previously been detected in sparrows. Neutralizing antibodies to USUV were present in the pooled sample from four great tits. Due to insufficient sample volumes, VNT for TBEV was not performed in the birds. The results further demonstrate the endemicity of WNV and other vector-borne flaviviruses in natural and accidental hosts in Slovakia, which adds to the overview of flavivirus epidemiology in European countries in general.
Other data
OCA16. Annotation of the output in English	In central Europe, at least three flaviviruses circulate among vectors and vertebrate hosts. West Nile virus (WNV) and Usutu virus (USUV) are mosquito-borne viruses maintained in the nature by enzootic cycle between mosquitoes and birds. Tick-borne encephalitis virus (TBEV) is a flavivirus causing annual human cases in Slovakia. The aim of this study is the prevalence assessment of flavivirus infections in horses (n = 145) and birds (n = 109) by enzyme-linked immunosorbent assay (ELISA) and confirmation by neutralization test (VNT). WNV antibodies have been detected in 11.7% of tested horses and 11.9% of tested birds and confirmed in 6.9% of horse and 9.2% of bird samples. None of the WNV seropositive or dubious horses had WNV IgM (ELISA), and none of the tested horses had USUV neutralizing antibodies. Autochthonous WNV infections have been confirmed in 16.7% of horses without international travelling history. Most of them were from western Slovakia with known endemic WNV transmission. An autochthonous WNV infection in a horse from highland area of Kremnické vrchy (central Slovakia) with unknown data of WNV circulation and without travelling history was detected. TBEV antibody was detected in 6.2% of horses and in 3.4% has been confirmed. In two horses, WNV and TBEV infection could not be distinguished. Confirmed WNV seropositive were eight raptors showing nonspecific signs or suffering from trauma, one white stork, and one house sparrow. The sparrow was caught in a locality in eastern Slovakia, where WNV RNA had been previously detected in sparrows. USUV neutralizing antibodies were present in pooled sample from four Eurasian great tits. Because of insufficient volume, TBEV VNT was not carried out in birds. Results further prove the endemicity of WNV and other vector-borne flaviviruses in natural and accidental hosts in Slovakia, giving better insight in flavivirus epidemiology in European countries in general.
OCA17. List of maximum 5 most significant citations corresponding to the output	LECOLLINET, Sylvie - PRONOST, Stephane - COULPIER, Muriel - BECK, Cecile - GONZALEZ, Gaelle - LEBLOND, Agnes - TRITZ, Pierre. Viral Equine Encephalitis, a Growing Threat to the Horse Population in Europe? In VIRUSES-BASEL, 2020, vol. 12, no. 1, pp., Registrované v: WoS; ZUBRIKOVA, Dana - WITTMANN, Maria - HONIG, Vaclav - SVEC, Pavel - VICHOVA, Bronislava - ESSBAUER, Sandra - DOBLER, Gerhard - GRUBHOFFER, Libor - PFISTER, Kurt. Prevalence of tick-borne encephalitis virus and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks in Lower Bavaria and Upper Palatinate, Germany. In TICKS AND TICK-BORNE DISEASES. ISSN 1877-959X, 2020, vol. 11, no. 3, pp., Registrované v: WoS AUERSWALD, Heidi - RUGET, Anne Sophie - LADREYT, Helena - IN, Saraden - MAO, Sokthearom - SORN, San - TUM, Sothyra - DUONG, Veasna - DUSSART, Philippe - CAPPELLE, Julien - CHEVALIER, Véronique. Serological Evidence for Japanese Encephalitis and West Nile Virus Infections in Domestic Birds in Cambodia. In Frontiers in Veterinary Science, 2020-01-29, 7, pp., Registrované v: Scopus CAMINO, E. - SCHMID, S. - WEBER, F. - POZO, P. - DE JUAN, L. - KOENIG, M. - CRUZ-LOPEZ, F. Detection of antibodies against tick-borne encephalitis flaviviruses in breeding and sport horses from Spain. In TICKS AND TICK-BORNE DISEASES. ISSN 1877-959X, 2020, vol. 11, no. 5, pp., Registrované v: WoS VIDANA, Beatriz - BUSQUETS, Nuria - NAPP, Sebastian - PEREZ-RAMIREZ, Elisa - JIMENEZ-CLAVERO, Miguel angel - JOHNSON, Nicholas. The Role of Birds of Prey in West Nile Virus Epidemiology. In VACCINES, 2020, vol. 8, no. 3, pp., Registrované v: WoS
OCA18. Characteristics of the output's impact on socio-economic practice	The aim of this study was the prevalence assessment of flavivirus infections in horses (n = 145) and birds (n = 109) by enzyme-linked immunosorbent assay (ELISA) and confirmation by neutralization test (VNT). WNV antibodies have been detected in 11.7 % of tested horses and 11.9 % of tested birds and confirmed in 6.9 % of horse and 9.2 % of bird samples. Autochthonous WNV infections have been confirmed in 16.7 % of horses without international travelling history. Confirmed WNV seropositive were eight raptors showing nonspecific signs or suffering from trauma, one white stork, and one house sparrow. The sparrow was caught in a locality in eastern Slovakia, where WNV RNA had been previously detected in sparrows. USUV neutralizing antibodies were present in pooled sample from four Eurasian great tits. This is the first evidence of USUV circulation in birds in the Slovak Republic. Results further prove the endemicity of WNV and other vector-borne flaviviruses in natural and accidental hosts in Slovakia, giving better insight in flavivirus epidemiology in European countries in general.
OCA19. Characteristics of the output and related activities' impact on the educational process	The present work documents the results of serological screening of arboviruses in equine and avian populations in the Slovak Republic and extends our knowledge of these zoonotic pathogens. The impact of the work will be reflected in the teaching of courses with epidemiological content.