| Basic data |
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| OCA1. Surname awarded to the assessed person | Sopková |
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| OCA2. Name awarded to the assessed person | Drahomíra |
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| OCA3. Degrees awarded to the assessed person | Assoc. prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6022 |
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| OCA5. Area of assessment | study programme veterinary morphology and physiology · III. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2020 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 202878 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioForm&sid=0675C5A17FC713092F9A30ED75 |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://pubmed.ncbi.nlm.nih.gov/31410766/ |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | ADM ANDREJČÁKOVÁ, Zuzana - SOPKOVÁ, Drahomíra - VLČKOVÁ, Radoslava - HERTELYOVÁ, Zdenka - GANCARČÍKOVÁ, Soňa - NEMCOVÁ, Radomíra. The application of Lactobacillus reuteri CCM 8617 and flaxseed positively improved the health of mice challenged with enterotoxigenic E. coli O149:F4. In Probiotics and Antimicrobial Proteins. ISSN 1867-1306, 2020, vol. 12, no. 3, p. 937-951. (2019: 3.533 - IF, 3.204 - IF 5y, 23 - H-index, 0.718 - SJR, Q3 - SJR Best Q, Q2 - JCR Best Q). |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://link.springer.com/article/10.1007/s12602-019-09578-x |
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| OCA14. Characteristics of the author's contribution | Preparation of the experiment, electrophoretic analysis, gas chromatography |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | The aim of our study was to monitor the effects of dietary synbiotics on experimentally infected mice. Sixty mice were divided into the following three groups: negative control group C1, positive control group C2 (mice infected with enterotoxigenic Escherichia coli O149: F4NAL) and experimental group LF (probiotic strain Lactobacillus reuteri CCM 8617RIF + 10% flaxseed + E. coli O149 F4NAL). The additives were administered for 42 days. We performed microbiological, hematological and biochemical analyzes, electrophoretic analysis of lactate dehydrogenase (LDH) isoenzymes and analysis of fatty acids using gas chromatography and isotachophoresis. We observed a higher number of lactobacilli in the jejunum and ileum, a lower number of Enterobacteriaceae representatives in the faeces of animals and an increased production of organic acids in the group fed synbiotics. Applied synbiotics led to an increase in n-3 metabolism at the expense of n-6 polyunsaturated fatty acid metabolism; thus, they affected the n-6: n-3 ratios and the ratio of eicosapentaenoic acid to arachidonic acid. In addition, the incorporation of n-3 polyunsaturated fatty acids into the cell membrane reduced the activity of LDH, transaminases and alkaline phosphatase. The results obtained in our study suggest a positive effect of continuous supplementation of a combination of probiotic cheese enriched with L. reuteri CCM 8617RIF and shredded flaxseed on the composition of the intestinal microflora and alleviation of the course of infection caused by the pathogenic bacterium E. coli O149: F4NAL. |
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| Other data |
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| OCA16. Annotation of the output in English | The aim of our study was to monitor the effects of dietary synbiotics on experimentally infected mice. Sixty mice were divided into the following three groups: negative control group C1, positive control group C2 (mice infected with enterotoxigenic Escherichia coli O149:F4NAL), and experimental group LF (Lactobacillus reuteri CCM 8617RIF + 10% flaxseed + E. coli O149:F4NAL). Supplements were administered for 42 days. Microbiological, hematological, and biochemical analyses, electrophoretic analysis of lactate dehydrogenase (LDH) isoenzymes, and analysis of fatty acids using gas chromatography and isotachophoresis were performed. We recorded higher numbers of jejunal and ileal lactic acid bacteria, lower Enterobacteriaceae counts in the feces of the animals, and an increased production of organic acids in the synbiotic-fed group. The supplements applied favored n−3 polyunsaturated fatty acid (PUFA) metabolism and inhibited n−6 PUFA metabolism; thus, they influenced the n−6 to n−3 and eicosapentaenoic to arachidonic acid ratios. Additionally, the incorporation of n−3 PUFAs to the cell membrane decreased the activity of LDH, transaminases, and alkaline phosphatase. Results obtained in our study indicate the positive effect of continuous supplementation of combination of probiotic cheese enriched with L. reuteri CCM 8617RIF and crushed flaxseed on composition of intestinal microflora and alleviation of the course of infection induced by pathogenic bacterium E. coli O149:F4NAL. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | |
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| OCA18. Characteristics of the output's impact on socio-economic practice | Synbiotics appear to be a suitable food supplement that can influence the development of the infectious process in the animal\'s body. The prebiotic component (flaxseed) helps the colonization of the intestine by healthy probiotics and thus improves the health of the individual. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The results of the work lead to new knowledge in connection with the use of synbiotics in overcoming experimentally induced infection of mice. The used methods such as electrophoresis, spectroscopic analysis, gas chromatography are used at the Department of Biology and Physiology in the teaching process and in acquiring the skills of graduates and doctoral students. |
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