ADC VDOVIAKOVÁ, Katarína - DANKO, Ján - KREŠÁKOVÁ, Lenka - ŠIMAIOVÁ, Veronika - PETROVOVÁ, Eva - NOVOTNÝ, Jaroslav - ŽERT, Zdeněk - KOĽVEK, Filip - VALOCKÝ, Igor - VARGA, Maroš - ŠPAKOVSKÁ, Tatiana - PRIBULA, Jozef - GAŠPÁREK, Miroslav - GIRETOVÁ, Mária - ŠTULAJTEROVÁ, Radoslava - MEDVECKÝ, Ľubomír. The morphological, clinical and radiological outputs of the preclinical study after treatment of the osteochondral lesions in the porcine knee model using implantation of scaffold based on the of calcium phosphate biocement. In Frontiers in Materials. ISSN 2296-8016, 2021, vol. 8, no., art. no. 746800, p. [1-14]. (2020: 3.515 - IF, 3.536 - IF 5y, Q2

ADC VDOVIAKOVÁ, Katarína - DANKO, Ján - KREŠÁKOVÁ, Lenka - ŠIMAIOVÁ, Veronika - PETROVOVÁ, Eva - NOVOTNÝ, Jaroslav - ŽERT, Zdeněk - KOĽVEK, Filip - VALOCKÝ, Igor - VARGA, Maroš - ŠPAKOVSKÁ, Tatiana - PRIBULA, Jozef - GAŠPÁREK, Miroslav - GIRETOVÁ, Mária - ŠTULAJTEROVÁ, Radoslava - MEDVECKÝ, Ľubomír. The morphological, clinical and radiological outputs of the preclinical study after treatment of the osteochondral lesions in the porcine knee model using implantation of scaffold based on the of calcium phosphate biocement. In Frontiers in Materials. ISSN 2296-8016, 2021, vol. 8, no., art. no. 746800, p. [1-14]. (2020: 3.515 - IF, 3.536 - IF 5y, Q2 - JCR Best Q).

Basic data
OCA1. Surname awarded to the assessed person	Valocký
OCA2. Name awarded to the assessed person	Igor
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6025
OCA5. Area of assessment	study programme veterinary obstetrics and gynecology · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2021
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	443047
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildS9OHM&sid=D2BE33474DF4BCB756855BFBDF&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000732267700001
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADC VDOVIAKOVÁ, Katarína - DANKO, Ján - KREŠÁKOVÁ, Lenka - ŠIMAIOVÁ, Veronika - PETROVOVÁ, Eva - NOVOTNÝ, Jaroslav - ŽERT, Zdeněk - KOĽVEK, Filip - VALOCKÝ, Igor - VARGA, Maroš - ŠPAKOVSKÁ, Tatiana - PRIBULA, Jozef - GAŠPÁREK, Miroslav - GIRETOVÁ, Mária - ŠTULAJTEROVÁ, Radoslava - MEDVECKÝ, Ľubomír. The morphological, clinical and radiological outputs of the preclinical study after treatment of the osteochondral lesions in the porcine knee model using implantation of scaffold based on the of calcium phosphate biocement. In Frontiers in Materials. ISSN 2296-8016, 2021, vol. 8, no., art. no. 746800, p. [1-14]. (2020: 3.515 - IF, 3.536 - IF 5y, Q2 - JCR Best Q).
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.frontiersin.org/articles/10.3389/fmats.2021.746800/full
OCA14. Characteristics of the author's contribution	Premedication of the animals and introduction of general anesthesia into the left ear vein (v. Auricularis sinistra) by insertion of a permanent intravenous cannula by administration of thiopental 15 mg / kg. Postoperative care by application of antibiotic prophylaxis with repeated doses of broad-spectrum antibiotics oxytetracycline dihydrate 1 ml / 10 kg b.w. (Alamycin LA auv, Norbrook, Newry, UK), intramuscularly every other day for 7 days and from repeated injections of the non-steroidal anti-inflammatory drug flunixin meglumine 2.2 mg / kg body weight (Flunixin auv, Norbrook, Newry, UK), intramuscular once a day for 7 days. Monitoring of animals daily in the postoperative period until the end of the experiment. Evaluation of the following parameters: general health, body temperature, stool consistency, degree of limb pain, surgical wound, signs of inflammation (redness, swelling, heat, discharge, pain) and degree of curvature. Animal euthanasia and collect sampling.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	We studied the potential of calcium phosphate-based biocement consisting of a mixture of four amino acids (glycine, proline, hydroxyproline and lysine) in the regeneration process of an artificially created osteochondral defect on a porcine medial femoral condyle in the knee joint.
Other data
OCA16. Annotation of the output in English	Full-thickness cartilage lesions or cartilage degeneration lead to destruction of the normal chondral architecture and bone structure in the affected area, causing osteoarthritis and general damage to health. The knee joints are most often affected by this condition. Permanent damage to articular cartilage and subchondral bone has motivated many scientists and physicians to investigate new methods of regenerating osteochondral defects, such as new materials. an artificial osteochondral defect on the porcine medial femoral condyle in the knee joint. The weight ratio of amino acids in CAL biocement was 4: 2: 2: 1. The Ca / P ratio in the cement was 1.67, which corresponds to the hydroxyapatite ratio. We compared the results with the spontaneous healing of an artificial cyst with healthy tissue. The group of animals treated with CAL biocement paste showed completely filled osteochondral defects. The results confirmed histological and radiological examinations, which showed regenerated chondral and bone tissue in the examined knee joints. Macroscopic evaluation showed that the cartilage was well integrated with the neighboring native cartilage in the group of animals with biocement CAL compared to artificial cyst healing, where the treated cartilage surfaces were visibly lower than the surrounding native cartilage surface and the boundary between native and regenerated tissue was clear. Qualitative evaluation of implant histological specimens showed complete regeneration of hyaline cartilage and subchondral bone in CAL-cemented animals. The artificial cyst group showed remarkable fibrillation. Detailed MRI analysis of the cross-section of the osteochondral defect confirmed complete healing of the cartilage and subchondral bone, where the thickness of the regenerated cartilage was 1.5 mm. MRI imaging of the defects in the artificial cyst group showed incomplete healing.
OCA17. List of maximum 5 most significant citations corresponding to the output
OCA18. Characteristics of the output's impact on socio-economic practice	An important fact is that chondral and bone tissue are closely related. In the treatment of osteochondral defects, it is therefore necessary to consider them as a complex of articular cartilage and subchondral bone, which have very well organized tissue. Further research into the therapy of osteochondral lesions should focus on improving the modification of skeletons, which will have the ability to create a physiological architecture of regenerated cartilage and subchondral bone, supporting their functional character and their mechanical properties.
OCA19. Characteristics of the output and related activities' impact on the educational process	Development of new knowledge in the study of bone marrow cells, growth factors and cytokines in scaffolds, which are important and interesting for proper repair processes in osteochondral lesions, their difficult storage, low cell survival and factor instability. In the group of animals treated with CAL biomaterial, the defect filled with new regenerated chondral and bone tissue and biocement was almost completely reabsorbed.