MRAVCOVÁ, Kristína (44%) - ŠTRKOLCOVÁ, Gabriela (32%) - MUCHA, Rastislav (5%) - BARBUŠINOVÁ, Eva (5%) - GOLDOVÁ, Mária (1%) - KAČÍROVÁ, Jana (5%) - MAĎAR, Marián (8%). Cryptosporidium parvum – zoonotic subtype IIdA15G1 in a Slovakian patient .In Annals of Agricultural and Environmental Medicine. - ISSN 1232-1966. - Vol. 27, no. 3 (2020), p. 485-488. (2019: 0.982 - IF, 1.226 - IF 5y, 51 - H-index, 0.366 - SJR, Q3 - SJR Best Q, Q4

MRAVCOVÁ, Kristína (44%) - ŠTRKOLCOVÁ, Gabriela (32%) - MUCHA, Rastislav (5%) - BARBUŠINOVÁ, Eva (5%) - GOLDOVÁ, Mária (1%) - KAČÍROVÁ, Jana (5%) - MAĎAR, Marián (8%). Cryptosporidium parvum – zoonotic subtype IIdA15G1 in a Slovakian patient .In Annals of Agricultural and Environmental Medicine. - ISSN 1232-1966. - Vol. 27, no. 3 (2020), p. 485-488. (2019: 0.982 - IF, 1.226 - IF 5y, 51 - H-index, 0.366 - SJR, Q3 - SJR Best Q, Q4 - JCR Best Q).

Basic data
OCA1. Surname awarded to the assessed person	Štrkolcová
OCA2. Name awarded to the assessed person	Gabriela
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/26622
OCA5. Area of assessment	study programme parasitic diseases of animals · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2020
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	205259
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildW1912&sid=CF17AC7264020AC9945F20B4DF&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.webofscience.com/wos/woscc/full-record/WOS:000569458100026
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	MRAVCOVÁ, Kristína (44%) - ŠTRKOLCOVÁ, Gabriela (32%) - MUCHA, Rastislav (5%) - BARBUŠINOVÁ, Eva (5%) - GOLDOVÁ, Mária (1%) - KAČÍROVÁ, Jana (5%) - MAĎAR, Marián (8%). Cryptosporidium parvum – zoonotic subtype IIdA15G1 in a Slovakian patient .In Annals of Agricultural and Environmental Medicine. - ISSN 1232-1966. - Vol. 27, no. 3 (2020), p. 485-488. (2019: 0.982 - IF, 1.226 - IF 5y, 51 - H-index, 0.366 - SJR, Q3 - SJR Best Q, Q4 - JCR Best Q).
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://link.springer.com/article/10.10070.000000s00436-016-4935-3
OCA14. Characteristics of the author's contribution	Co-author; the bearer of ideas in the article.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	In order to confirm the suspected cryptosporidiosis, the ELISA test was used for proof of the coproantigen. The coproantigen was detected by means of a commercially available kit, according to the manufacturer’s instructions. A faecal smear was prepared to investigate the presence of Cryptosporidium oocysts. The samples were stained with a special acid-resistant stain according to Ziehl–Neelsen. DNA was extracted from the fecal sample using the ZR Fecal DNA MiniPrep™ kit according to manufacturer`s instructions, and then subjected to a nested PCR. Na identifikáciu Cryptosporidium spp. vo vzorkách stolice sa pomocou nested PCR sme amplifikovali fragment pokrývajúci 18S rRNA a gén GP60.
Other data
OCA16. Annotation of the output in English	The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. Until now, 38 valid species of Cryptosporidium genus have been described; C. hominis and C.parvum are responsible for most human infections. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified./ Parazit Cryptosporidium spp. je intracelulárny protozoárny prvok, ktorý má široký rozsah hostiteľov a zoonotický potenciál. V súčasnosti bolo opísaných 38 platných druhov z rodu Cryptosporidium, z ktorých viac ako 20 bolo hlásených u ľudí; C. hominis a C. parvum sú zodpovedné za väčšinu ľudských infekcií. Na potvrdenie prítomnosti oocýst Cryptosporidium bola použitá Ziehl-Neelsenova farbiaca technika. Na detekciu koproantigénu vo vzorkách stolice bol použitý test ELISA. Pomocou nested PCR sme amplifikovali malú ribozomálnu podjednotku( SSU rRNA), čím sme potvrdili druh C. parvum a následne pomocou sekvenácie génu GP60 sme určili zoonotický subtyp IIdA15G1 bol identifikovaný ako zoonotický podtyp.
OCA17. List of maximum 5 most significant citations corresponding to the output	Emergence of zoonotic Cryptosporidium parvum in China / Guo, Yaqiong [Autor, 25%] ; Ryan, Una [Autor, 25%] ; Feng, Yaoyu [Autor, 25%] ; Xiao, Lihua [Autor, 25%]. – DOI 10.1016/j.pt.2021.12.002. – WOS CC In: Trends in Parasitology [textový dokument (print)] . – Londýn (Veľká Británia) : Elsevier. – ISSN 1471-4922. – ISSN (online) 1471-5007. – Roč. 38, č. 4 (2022), s. 335-343 [tlačená forma]A, Corinne - FIDELLE, Francis - GRISEZ, Christelle - PREVOT, Francoise - JACQUIET, Philippe - RAZAKANDRAINIBE, Romy. Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum. In PARASITE. ISSN 1252-607X, 2020, vol. 27, no., pp., Registrované v: WoS Clinical cases of zoonotic Cryptosporidium parvum (subtype IIdA15G1) infections in Korean goats / Kim, Jongho [Autor, 14.286%] ; Lee, Kyunghyun [Autor, 14.286%] ; Roh, Su Gwon [Autor, 14.286%] ; Kim, Ha-Young [Autor, 14.286%] ; Kim, Jong Wan [Autor, 14.285%] ; So, Byungjae [Autor, 14.285%] ; Choi, Eun-Jin [Autor, 14.286%]. – DOI 10.17221/212/2020-VETMED. – WOS CC In: Veterinární medicína [textový dokument (print)] [elektronický dokument] : mezinárodní vědecký časopis vydávaný z pověření Ministerstva zemědělství České republiky a pod gescí České akademie zemědělských věd. – Praha (Česko) : Česká akademie zemědělských věd. – ISSN 0375-8427. – ISSN (zrušené) 0590-5214. – Roč. 67, č. 3 (2022), s. 156-162 [tlačená forma] [online] A survey of Cryptosporidium prevalence among birds in two zoos in China / Lu, Yaxian [Autor, 5.263%] ; Pu, Tianchun [Autor, 5.263%] ; Ma, Baohua [Autor, 5.263%] ; Wang, Lixin [Autor, 5.263%] ; Zhou, Mengchao [Autor, 5.263%] ; Chen, Yu [Autor, 5.263%] ; Li, Xiuyun [Autor, 5.263%] ; Zheng, Changming [Autor, 5.263%] ; Liu, Hetong [Autor, 5.263%] ; Liu, Jinpeng [Autor, 5.263%] ; Guan, Chunyu [Autor, 5.263%] ; Yu, Hongyan [Autor, 5.263%] ; Dai, Chunkuo [Autor, 5.263%] ; Huang, Yuan [Autor, 5.264%] ; Yang, Yuling [Autor, 5.263%] ; Peng, Zhiwei [Autor, 5.264%] ; Han, Lei [Autor, 5.263%] ; Chai, Hongliang [Autor, 5.264%] ; Hou, Zhijun [Autor, 5.263%]. – DOI 10.7717/peerj.12825. – WOS CC In: PeerJ [elektronický dokument] . – Londýn (Veľká Británia) : Peerj INC. – ISSN (online) 2167-8359. – Roč. 10 (2022), s. [online] Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in Central Inner Mongolia, Northern China / Zhao, Li [Autor, 6.667%] ; Chai, Hai-Liang [Autor, 6.667%] ; Wang, Ming-Yuan [Autor, 6.667%] ; Zhang, Zhan-Sheng [Autor, 6.667%] ; Han, Wen-Xiong [Autor, 6.667%] ; Yang, Bo [Autor, 6.666%] ; Wang, Yan [Autor, 6.667%] ; Zhang, Shan [Autor, 6.666%] ; Zhao, Wei-Hong [Autor, 6.667%] ; Ma, Yi-Min [Autor, 6.666%] ; Zhan, Yong-Jie [Autor, 6.667%] ; Wang, Li-Feng [Autor, 6.666%] ; Ding, Yu-Lin [Autor, 6.667%] ; Wang, Jin-Ling [Autor, 6.666%] ; Liu, Yong-Hong [Autor, 6.667%]. – DOI 10.1186/s12917-023-03696-z. – WOS CC In: BMC Veterinary Research [elektronický dokument] . – Londýn (Veľká Británia) : BMC. – ISSN (online) 1746-6148. – Roč. 19, č. 1 (2023), s. [online] Prevalence and molecular characterization of Cryptosporidium spp., Enterocytozoon bieneusi, and Giardia duodenalis in dairy cattle in Ningxia, northwestern China / Wang, Ming-Yuan [Autor, 8.333%] ; Zhang, Shan [Autor, 8.333%] ; Zhang, Zhan-Sheng [Autor, 8.333%] ; Qian, Xiao-Yin [Autor, 8.333%] ; Chai, Hai-Liang [Autor, 8.334%] ; Wang, Yan [Autor, 8.333%] ; Fan, Wen-Jun [Autor, 8.334%] ; Yi, Chao [Autor, 8.333%] ; Ding, Yu-Lin [Autor, 8.334%] ; Han, Wen-Xiong [Autor, 8.333%] ; Zhao, Li [Autor, 8.334%] ; Liu, Yong-Hong [Autor, 8.333%]. – DOI 10.1007/s11259-024-10364-6. – WOS CC In: Veterinary research communications [textový dokument (print)] [elektronický dokument] . – Amsterdam (Holandsko) : Springer Nature. – ISSN 0165-7380. – ISSN (online) 1573-7446. – Roč. 48, č. 4 (2024), s. 2629-2643 [tlačená forma] [online]
OCA18. Characteristics of the output's impact on socio-economic practice	The output reveals an information on epidemiology of cryptosporidiosis in Slovakia and the spread of this infection from animals to humans. Recognition of subtypes allows identification of the source of infection. On this basis, it is possible to develop measures to protect the health of the population and prevent the spread of this infection. . The presented case report indicates a potential danger of outbreaks of zoonotic cryptosporidiosis and the need for strict control and hygiene practices during the handling of young animals, especially calves.
OCA19. Characteristics of the output and related activities' impact on the educational process	The output describes our procedure for the identification of the oocysts and subtypes of Cryptosporidium spp. The procedure includes acid-resistant Ziehl-Nielsen staining to confirm oocysts, ELISA method to detect coproantigen, and the PCR method to confirm species and subtypes. Microscopically positive specimens are used for genotyping of Cryptosporidium spp., as well as the bioinformatics analysis of the obtained data. The methodology of this article can be used in the educational process. Also, all methodologies can be a model for the confirmation o of parasites during the solution of diploma and dissertation theses.