ADN - MARCIN, Andrej - NAĎ, Pavel – SKALICKÁ, Magdaléna – BUJŇÁK, Lukáš – GANCARČÍKOVÁ, Soňa. Effect of humic substances on proteolytic activity in intestine, digestibility of crude protein and protein content in the blood of broiler chickens. In Acta fytotechn zootechn. ISSN 1336-9245, 2021, roč. 24, (Monothematic Issue: Problems and Risks in Animal Production), s. 159-163.

Basic data
OCA1. Surname awarded to the assessed person	Marcin
OCA2. Name awarded to the assessed person	Andrej
OCA3. Degrees awarded to the assessed person	Assoc. prof. , B.Sc., DVM, CSc.
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6166
OCA5. Area of assessment	study programme nutrition of animals and dietetics · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2021
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID 247111
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=20124479BBF29892273D79E970
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.scopus.com/record/display.uri?eid=2-s2.0-85102351089&origin=resultslist&sort=plf-f&src=s&sid=f73399d4d0ee20b0613eb08deb7c317b&sot=b&sdt=b&sl=163&s=TITLE-ABS-KEY%28Effect+of+humic+substances+on+proteolytic+activity+in+intestine%2c+digestibility+of+crude+protein+and+protein+content+in+the+blood+of+broiler+chickens%29&relpos=0&citeCnt=0&searchTerm=
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADN - MARCIN, Andrej - NAĎ, Pavel – SKALICKÁ, Magdaléna – BUJŇÁK, Lukáš – GANCARČÍKOVÁ, Soňa. Effect of humic substances on proteolytic activity in intestine, digestibility of crude protein and protein content in the blood of broiler chickens. In Acta fytotechn zootechn. ISSN 1336-9245, 2021, roč. 24, (Monothematic Issue: Problems and Risks in Animal Production), s. 159-163.
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	http://acta.fapz.uniag.sk/journal/index.php/on_line/article/view/878
OCA14. Characteristics of the author's contribution	The first author performed: 1) the quantification of proteolytic activity in the GIT of broiler chickens, 2) the analysis of nitrogen digestibility (NL) measured as apparent assimilable mass coefficient CP corrected for protein catabolism (AMCN).
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	It was performed an experimental feeding study with broiler chickens Cobb 500 (n = 120). The evaluation of dietary effects of HS on proteolytic activity and digestibility of CP in the chymus of the gut and the protein content of the blood serum was performed. Birds (groups A, B, C - negative control) were fed with mixtures with CP (g/kg DM) – Hyd1 230.20 (d1–7), Hyd2 222.20 (d8–28), Hyd3 209.40 (d29–37). HS were added into diets of experimental groups according to content of humic/fulvic acids (HA/FA; g/kg) A 4.55/0.35, B 3.99/0.35, C 2.85/0.25. The body weights and feed consumption were measured once a week. The average daily weight gains and the feed conversion ratio were calculated. The intake of HS had a positive effect on the increase of proteolytic activities in the intestinal apparatus and caused a significant enhancement of AMCN of birds from experimental groups on days 17, 24 and 31. The values of the total protein and the albumin in the serum were significantly decreased in the groups after intake of HA/FA in A and B groups.
Other data
OCA16. Annotation of the output in English	The study aimed to investigate the effects of dietary intake of humic substances (HS) on the proteolytic activity and the digestibility of crude protein (CP) measured as the apparent assimilable mass coefficient of CP corrected for protein catabolism (AMCN) in the intestine as well as on the total protein and albumin content in the serum of broiler chickens Cobb 500 (n =120). Birds (groups A, B, C - negative control) were fed with mixtures with CP (g/kg DM) – Hyd1 230.20 (d1–7), Hyd2 222.20 (d8–28), Hyd3 209.40 (d29–37). HS were added into diets of experimental groups according to the content of humic/fulvic acids (HA/FA; g/kg) A 4.55/0.35, B 3.99/0.35, C 2.85/0.25. The body weights and feed consumption were measured once a week. The average daily weight gains and the feed conversion ratio were calculated. The intake of HS had a positive effect on the increase of proteolytic activities in the intestinal apparatus and caused a significant enhancement of AMCN of birds from experimental groups on days 17, 24 and 31. The values of the total protein and the albumin in the serum were significantly decreased in the groups after intake of HA/FA in the A and B groups.
OCA17. List of maximum 5 most significant citations corresponding to the output
OCA18. Characteristics of the output's impact on socio-economic practice	Experimental results of testing the effects of humic substances in Cobb 500 broiler chickens with the intention to influence their proteolytic activity and digestibility in the chymus of the intestinal tract, as well as protein content in the blood serum of chickens have the potential to be used in farm conditions of broiler chickens. better use of feed.
OCA19. Characteristics of the output and related activities' impact on the educational process	The methodology and the experimental results of testing of the effects of humic substances with Cobb 500 broiler chickens with the intention to determine their influence on proteolytic activity and digestibility in intestinal chemistry, as well as protein content in the blood serum of chickens are usable in the theoretical educational process and also in the experimental activities of students in the preparation of final theses.