ADE - MARCIN, Andrej - NAĎ, Pavel - BUJŇÁK, Lukáš - SKALICKÁ, Magdaléna. Digestibility and performance of broiler chickens fed diets with humic substances. In: Animal Welfare, Ethology and Housing Systems. 2020. ISSN 1786-8440, Vol. 16, no. 2 (2020), p. 137-145.

Basic data
OCA1. Surname awarded to the assessed person	Marcin
OCA2. Name awarded to the assessed person	Andrej
OCA3. Degrees awarded to the assessed person	Assoc. prof. , B.Sc., DVM, CSc.
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6166
OCA5. Area of assessment	study programme feed and foodstuff safety · I. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2020
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID: 230029
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=1447EAD9E9ED6D3C2273565EB0
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	DOI10.17205/SZIE.AWETH.2020.2.137
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADE - MARCIN, Andrej - NAĎ, Pavel - BUJŇÁK, Lukáš - SKALICKÁ, Magdaléna. Digestibility and performance of broiler chickens fed diets with humic substances. In: Animal Welfare, Ethology and Housing Systems. 2020. ISSN 1786-8440, Vol. 16, no. 2 (2020), p. 137-145.
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	http://real.mtak.hu/118809/1/AWETH202002137145_doi.pdf
OCA14. Characteristics of the author's contribution	The first author performed laboratory analyses of enzymatic activity in the GIT of chickens, laboratory analyses of digestibility of nitrogenous substances in the GIT of chickens, and statistical analysis.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The objective of the study was to investigate the effects of dietary intake of humic substances (HS) on the ileal digestibility of dry matter (DM) and crude protein (CP). One-day-old broiler chickens (Cobb 500, n=120, average weight 50 g) were divided at random into 4 equal groups (A, B, C / negative control). Chickens were fed with mixtures with the content of CP - BR1 230.20, BR2 222.20, BR3 209.40 g.kg-1 DM for 37 days. The applied additives HN/HNM (Humac Ltd., Slovak Republic) contained humic acids (HA) min. 650/570 and fulvic acids (FA) min. 50/50 g.kg-1. The humic substances were added into diets of experimental groups according to the content of HA/FA in the feed additives A 4.55/0.35, B 3.99/0.35, C 2.85/0.25 g.kg-1. The body weights (BW) and feed consumption were assessed once a week. The average daily weight gains (ADWG), average daily feed intake (ADFI) and the feed conversion ratio (FCR) were calculated. The ileal chymus, sampled from 48 birds after slaughter, was used for the quantification of DM, CP, ash and ash insoluble in HCl on day 37. The production parameters of chickens were from day 1 – 37 as follows. The average finishing body weights were A 2506.67 ± 160.47, B 2490.25 ± 166.39, C 2377.75 ± 133.19 and control 2319.42 ± 92.55 g. The average weight gains achieved the values A 66.39 ± 16.85, B 65.95 ± 17.77, C 62.91 ± 18.06 and control 61.34 ± 17.31 g.day-1. The average daily feed intakes were A 108.97 ± 50.11, B 116.91 ± 55.35, C 108.85 ± 51.44 and control 106.83 ± 46.97 g.day-1 from day 1 – 37. The addition of humic substances caused the increase of ileal digestibility in B group compared to control: DM 38.32 ± 0.18 / 30.94 ± 2.41% (p < 0.05) and CP 40.39 ± 0.05 / 34.29 ± 0.26% (p < 0.05). The dietary intake of feed additives containing HS seems to have a positive effect on finishing BW and the ileal digestibility of DM and CP in the case of the minimal inclusion level of HA/FA 3.99/0.35 g.kg-1.
Other data
OCA16. Annotation of the output in English	The dietary intake of HS seems to have a positive effect on the ileal digestibility at the inclusion level HA/FA at minimum 3.99/0.35 g.kg-1. There were observed the increased digestibility of DM by 7.38% (p < 0.05) and CP by 6.1% (p < 0.05) compared to negative control. The finishing individual BW of experimental groups were positively influenced in A by 187.25 (p < 0.05) and in B groups by 170.83 g (p < 0.05).
OCA17. List of maximum 5 most significant citations corresponding to the output
OCA18. Characteristics of the output's impact on socio-economic practice	Dietary humic substances (HS) intake had a positive effect on increasing proteolytic activities in the intestinal tract and caused a significant increase in AMCN in birds from the experimental groups. However, the values of total protein and serum albumin were significantly reduced in the group after adding HA/FA 4.55/0.35 or 3.99/0.35 to the feed.
OCA19. Characteristics of the output and related activities' impact on the educational process	Methodology of a feeding trial with broiler chickens using humic substances (HS) with the potential to influence digestibility and proteolytic activity in the GIT. The methodology can be implemented in the educational process at the 1st level of university studies as a model of its practical use.