TARKO, Adam - FABOVÁ, Zuzana - KOTWICA, Jan - VALOCKÝ, Igor - ALREZAKI, Abdulkarem - ALWASEL, Saleh - HARRATH, Abdel Halim - SIROTKIN, Alexander V. The inhibitory influence of toluene on mare ovarian granulosa cells can be prevented by fennel. In General and comparative endocrinology. ISSN 1095-6840, 2020, vol. 295, no, art. no. 113491, p. [1-6]. (2020: 2.822 - IF, 2.772 - IF 5y, 119 - H-index, 0.819 - SJR, Q1 - SJR Best Q, Q3 - JCR Best Q). (2020

TARKO, Adam - FABOVÁ, Zuzana - KOTWICA, Jan - VALOCKÝ, Igor - ALREZAKI, Abdulkarem - ALWASEL, Saleh - HARRATH, Abdel Halim - SIROTKIN, Alexander V. The inhibitory influence of toluene on mare ovarian granulosa cells can be prevented by fennel. In General and comparative endocrinology. ISSN 1095-6840, 2020, vol. 295, no, art. no. 113491, p. [1-6]. (2020: 2.822 - IF, 2.772 - IF 5y, 119 - H-index, 0.819 - SJR, Q1 - SJR Best Q, Q3 - JCR Best Q). (2020 - Current Contents).

Basic data
OCA1. Surname awarded to the assessed person	Valocký
OCA2. Name awarded to the assessed person	Igor
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6025
OCA5. Area of assessment	study programme general veterinary medicine · I. + II. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2020
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	186928
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildODS05&sid=9EB7DDC054CB7EBFE17AB5FCE5&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://arl4.library.sk/arl-uvlk/sk/detail-uvlk_un_cat-0034799-The-inhibitory-influence-of-toluene-on-mare-ovarian-granulosa-cells-can-be-prevented-by-fennel/?disprec=1&iset=3
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	TARKO, Adam - FABOVÁ, Zuzana - KOTWICA, Jan - VALOCKÝ, Igor - ALREZAKI, Abdulkarem - ALWASEL, Saleh - HARRATH, Abdel Halim - SIROTKIN, Alexander V. The inhibitory influence of toluene on mare ovarian granulosa cells can be prevented by fennel. In General and comparative endocrinology. ISSN 1095-6840, 2020, vol. 295, no, art. no. 113491, p. [1-6]. (2020: 2.822 - IF, 2.772 - IF 5y, 119 - H-index, 0.819 - SJR, Q1 - SJR Best Q, Q3 - JCR Best Q). (2020 - Current Contents).
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.sciencedirect.com/science/article/pii/S0016648020300290
OCA14. Characteristics of the author's contribution	Participation in the experimental part, evaluation of the achieved results and participation in the final editing of the publication.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	This study aimed to examine the action of toluene (20 μg/mL) and fennel extract (0, 1, 10, 100 μg/mL), and assess their combination on viability, proliferation, apoptosis, and hormone release by cultured healthy mare ovarian granulosa cells. Viability, proliferation (percentage of PCNA-positive cells), apoptosis and release of progesterone, oxytocin and prostaglandin F were evaluated by using Trypan blue exclusion tests, immunocytochemistry and enzyme immunoassays, respectively. Toluene, when given alone, inhibited viability, proliferation, apoptosis, progesterone, prostaglandin F and IGF-I. However, it did not affect oxytocin release. Moreover, fennel, when given alone, inhibited viability, progesterone, and prostaglandin F release, as well as stimulating proliferation and oxytocin release.
Other data
OCA16. Annotation of the output in English	Our observations provide the first evidence that fennel can be potential for the prevention of adverse effects of toluene on ovarian cell viability, proliferation, apoptosis, and prostaglandin F and IGF-I release. All these processes are necessary for successful reproduction . Therefore, their damage by toluene can negatively affect reproductive processes, whilst fennel could be a natural protector against toluene negative effects. This could be sustained to improve the reproductive function of mares, even in conditions of environmental contamination by oil products.
OCA17. List of maximum 5 most significant citations corresponding to the output	ZHONG, Lulu - LUO, Yue - ZHOU, Feng - YANG, Xuebin - TAN, Yang - LI, Shunmin - PEI, Gang. The effects of natural products and bioactive ingredients of traditional Chinese medicine on apoptosis of ovarian granulosa cells. In Journal of Applied Toxicology, 2023-06-01, 43, 6, pp. 772-788. ISSN 0260437X. Dostupné na: https://doi.org/10.1002/jat.4406., Registrované v: SCOPUS
OCA18. Characteristics of the output's impact on socio-economic practice	Our observations are the first demonstration that fennel can be used as a natural protector of ovarian cells against the action of toluene, and maybe other oil-related environmental contaminants. Therefore, application of fennel as natural protector against adverse action of oil-related contaminants on reproductive processes in other animal species and humans cannot be excluded.
OCA19. Characteristics of the output and related activities' impact on the educational process	The publication is suitable study literature for students of the general veterinary medicine study program.