| Basic data |
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| OCA1. Surname awarded to the assessed person | Mudroňová |
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| OCA2. Name awarded to the assessed person | Dagmar |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6094 |
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| OCA5. Area of assessment | study programme microbiology and immunology · III. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2025 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 1307809 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioForm&sid=3113E0813724FF86377677BE7BA3 |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://pubmed.ncbi.nlm.nih.gov/39955331/ |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | ID: 1307809 | Flavonolignans silybin, silychristin and 2,3-dehydrosilybin showed differential cytoprotective, antioxidant and anti-apoptotic effects on splenocytes from Balb/c mice / Jurčacková, Zuzana [Autor, 20%] ; Hrčková, Gabriela [Autor, 20%] ; Mudroňová, Dagmar [Autor, 20%] ; Alexovič Matiašová, Anna [Autor, 20%] ; Biedermann, David [Autor, 20%]. – [recenzované]. – DOI 10.1038/s41598-025-89824-1. – WOS CC ; CCC |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://www.nature.com/articles/s41598-025-89824-1 |
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| OCA14. Characteristics of the author's contribution | The author is one of the bearers of the idea of the output and at the same time the implementer and creator of the output. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | There is a limited amount of information about the effects of individual flavonolignans on immune cells. Therefore, we focused on evaluating the antioxidant, cytoprotective and anti-apoptotic effects of silybin (SB), silychristin (SCH) and 2,3-dehydrosilybin (DHSB) on the spleen cells isolated from healthy Balb/c mice by monitoring viability, changes in mitochondrial activity and apoptosis as well as the effect of substances on the production of free oxygen and nitrogen radicals, H2O2 and changes in the expression of selected genes with a role in endogenous antioxidant mechanisms. A cytoprotective effect of SB, SCH and DHSB was observed at concentrations as low as 5 µmol/ml. At 50 µmol/ml, these substances restored and/or stimulated viability and mitochondrial membrane potential and had anti-apoptotic effect in the order SB > DHSB > SCH. The substances demonstrated a concentration-dependent activity in restoring the redox balance in the order DHSB > SCH > SB, which correlated with the suppressed expression of nuclear factor Nrf2, catalase and glutathione peroxidase. Our study showed significant differences in the cytoprotective, antioxidant and anti-apoptotic activities of SB, SCH, and DHSB on splenocytes exposed to mild and AAPH-induced oxidative stress. |
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| Other data |
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| OCA16. Annotation of the output in English | There is a limited amount of information about the effects of individual flavonolignans on immune cells. In our work, we, therefore, focused on evaluating the antioxidant, cytoprotective and anti-apoptotic effects of silybin, silychristn and 2,3-dehydrosilybin on the spleen cells isolated from healthy Balb/c mice by monitoring viability, changes in mitochondrial activity and apoptosis as well as the effect of substances on the production of free oxygen and nitrogen radicals, H2O2 and changes in the expression of selected genes with a role in endogenous antioxidant mechanisms. Our study showed significant differences in the cytoprotective, antioxidant and anti-apoptotic activities of SB, SCH, and DHSB on splenocytes exposed to mild and AAPH-induced oxidative stress. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The result of the published research was the acquisition of new knowledge about the mechanism of the effects of flavolignans on immune cells, the determination of the antioxidant, cytoprotective and antiapoptotic activities of the individual components of the silymarin complex under study, as well as effective concentrations. Since silymarin or its components are part of drugs used mainly for liver regeneration and protection, this new knowledge will contribute to the development of new, even more effective and precisely targeted drugs. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The output brings new knowledge about the mode of action of flavolignans on immune cells, about the antioxidant, cytoprotective and antiapoptotic efficacy of the individual components of the silymarin complex, as well as about their effective concentrations. This knowledge will be used for further education of students in the 3rd cycle of university studies in the Immunology program. |
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