| Basic data |
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| OCA1. Surname awarded to the assessed person | Mudroňová |
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| OCA2. Name awarded to the assessed person | Dagmar |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6094 |
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| OCA5. Area of assessment | study programme microbiology and immunology · III. degree |
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| OCA6. Category of the research/artistic/other output | |
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| OCA7. Year of publication of the research/artistic/other output | 2021 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 431183 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioForm&sid=117E8DF36B6BB49F0650DF69F7 |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://pubmed.ncbi.nlm.nih.gov/34687878/ |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | ID: 431183 | Effect of autochthonous lactobacilli on immunologically important molecules of rainbow trout after bacterial infection studied on intestinal primoculture / Cingeľová Maruščáková, Ivana [Autor, 30%] ; Schusterová, Petra [Autor, 15%] ; Popelka, Peter [Autor, 10%] ; Gancarčíková, Soňa [Autor, 10%] ; Csank, Tomáš [Autor, 5%] ; Fečkaninová, Adriána [Autor, 5%] ; Ratvaj, Marek [Autor, 5%] ; Mudroňová, Dagmar [Autor, 20%]. – DOI 10.1016/j.fsi.2021.10.021. – WOS CC ; SCO ; CCC
In: Fish & shellfish immunology [textový dokument (print)] [elektronický dokument] . – Londýn (Veľká Británia) : Elsevier. Academic Press. – ISSN 1050-4648. – ISSN (online) 1095-9947. – č. 119 (2021), s. 379-383 [tlačená forma] [online] |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://pubmed.ncbi.nlm.nih.gov/34687878/ |
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| OCA14. Characteristics of the author's contribution | The author is one of the bearers of the main idea of the output and at the same time the implementer and creator of the output. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | The output was focused on studying the influence of the autochthonous trout probiotic strain Lactobacillus plantarum R2 on the immune response of trout intestinal primoculture cells, both uninfected and infected with significant bacterial pathogens of salmonid fish - Aeromonas salmonicida subsp. salmonicida and Yersinia ruckeri. Tested probiotic strain reduced inflammation after A. salmonicida infection through decreased expression of pro-inflammatory cytokines and increased expression of anti-inflammatory cytokines. In contrast, after infection with Y. ruckeri, which causes immunosuppression, the probiotic strain stimulated immunity by up-regulation of expression of proinflammatory cytokines and suppressed the expression of anti-inflammatory cytokines. These results obtained on cell cultures are a prerequisite for the immunomodulatory potential of the strain, which was subsequently confirmed in in vivo experiments on trout and salmon. A significant contribution of the study was the introduction of the preparation of intestinal fish primocultures in the workplace. |
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| Other data |
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| OCA16. Annotation of the output in English | The aim of this work was to study the influence of probiotic bacteria on the immune response of trout intestinal cells in primoculture infected with pathogenic bacteria. In the experiment, we tested the effect of pre-treatment of intestinal cells with an autochthonous strain of Lactobacillus plantarum R2 Biocenol™ (CCM 8674) following infection with the most serious salmonid pathogens – Aeromonas salmonicida subsp. salmonicida (CCM 1307) and Yersinia ruckeri (CCM 6093). Tested probiotic strain reduced inflammation after A. salmonicida infection through decreased expression of pro-inflammatory cytokines and increased expression of anti-inflammatory cytokines. In contrast, after infection with Y. ruckeri, which causes immunosuppression, the probiotic strain stimulated immunity by up-regulation of expression of proinflammatory cytokines and suppressed the expression of anti-inflammatory cytokines. These results are a prerequisite for the immunomodulatory potential of the strain, but its action must be confirmed in subsequent in vivo experiments. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | Liu ZY, Yang HL, Wei CY, Cai GH, Ye JD, Zhang CX, Sun YZ. Commensal Bacillus siamensis LF4 induces antimicrobial peptides expression via TLRs and NLRs signaling pathways in intestinal epithelial cells of Lateolabrax maculatus. Fish Shellfish Immunol. 2023 Mar;134:108634. doi: 10.1016/j.fsi.2023.108634. |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The results obtained by testing the autochthonous probiotic strain on trout intestinal primary cells were the basis for the clarification of its immunomodulatory effect and the basis for subsequent in vivo experiments on rainbow trout and Atlantic salmon. As part of the solution of the experiments on the above-mentioned fish species, close cooperation was developed with Nord University in Bodo, Norway, as well as with the Pharmaceutical Faculty of Masaryk University in Brno, The Veterinary Reasearch Institute in Brno, the Veterinary University in Brno, and the Mendel University in Brno. The result of the above-mentioned cooperation is, in addition to joint scientific outputs, two patent applications: a joint Slovak-Norwegian one for probiotic feed for salmonids and a Slovak-Czech one for the technology for preparing probiotic feed for aquatic animals. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The output brings new knowledge about the influence of probiotic bacteria on the immune answer of trout intestinal cells. This knowledge will be used for further education of students in the 3rd level of university studies in the Microbiology and immunology program as well as students of veterinary medicine. |
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