ID: 177599 | The effect of humic substances on gut microbiota and immune response of broilers / Mudroňová, Dagmar [Autor, 25%] ; Karaffová, Viera [Autor, 25%] ; Pešulová, Terézia [Autor, 10%] ; Koščová, Jana [Autor, 10%] ; Cingeľová Maruščáková, Ivana [Autor, 5%] ; Bartkovský, Martin [Autor, 3%] ; Marcinčáková, Dana [Autor, 10%] ; Ševčíková, Zuzana [Autor, 2%] ; Marcinčák, Slavomír [Autor, 10%]. – DOI 10.1080/09540105.2019.1707780. – WOS CC ; SCO ; CCC In: Food and Agricultural Immunology [textový dokument (print)] [elektronický dokument] . – Abingdon (Veľká Británia) : Taylor & Francis Group. – ISSN 0954-0105. – ISSN (online) 1465-3443. – Roč. 31, č. 1 (2020), s. 137-149 [tlačená forma] [online]

Basic data
OCA1. Surname awarded to the assessed person	Mudroňová
OCA2. Name awarded to the assessed person	Dagmar
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6094
OCA5. Area of assessment	study programme microbiology and immunology · III. degree
OCA6. Category of the research/artistic/other output
OCA7. Year of publication of the research/artistic/other output	2020
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	177599
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=5F1CDE603F7696E939FCF77A83
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.scopus.com/record/display.uri?eid=2-s2.0-85077305212&origin=resultslist&sort=plf-f&src=s&st1=Mudronova&st2=dagmar&nlo=1&nlr=20&nls=afprfnm-t&sid=5341386c82bacfb580142a76233bb2cb&sot=anl&sdt=aut&sl=37&s=AU-ID%28%22Mudro%c5%88ov%c3%a1%2c+Dagmar%22+6507268940%29&relpos=21&citeCnt=9&searchTerm=&featureToggles=FEATURE_NEW_DOC_DETAILS_EXPORT:1
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ID: 177599 \| The effect of humic substances on gut microbiota and immune response of broilers / Mudroňová, Dagmar [Autor, 25%] ; Karaffová, Viera [Autor, 25%] ; Pešulová, Terézia [Autor, 10%] ; Koščová, Jana [Autor, 10%] ; Cingeľová Maruščáková, Ivana [Autor, 5%] ; Bartkovský, Martin [Autor, 3%] ; Marcinčáková, Dana [Autor, 10%] ; Ševčíková, Zuzana [Autor, 2%] ; Marcinčák, Slavomír [Autor, 10%]. – DOI 10.1080/09540105.2019.1707780. – WOS CC ; SCO ; CCC In: Food and Agricultural Immunology [textový dokument (print)] [elektronický dokument] . – Abingdon (Veľká Británia) : Taylor & Francis Group. – ISSN 0954-0105. – ISSN (online) 1465-3443. – Roč. 31, č. 1 (2020), s. 137-149 [tlačená forma] [online]
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.tandfonline.com/doi/full/10.1080/09540105.2019.1707780
OCA14. Characteristics of the author's contribution	The author is one of the bearers of the main idea of the output and at the same time the implementer and creator of the output.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The work was focused on the study of the immunomodulatory and gut-protecting effect of humic substances (HS) in broilers. The diet of experimental chicks was enriched with 0.8% of HS. We noted that HS had a stimulatory effect on the phagocytic activity and the engulfing capacity of phagocytes, however, the level of oxidative burst of phagocytes was not affected. We observed a significant increase of CD4+: CD8+ lymphocyte ratio, an indicator of immune stimulation. HS did not influence the IgA gene expression. In contrast, we observed a significant increase in the expression of MUC-2 (intestinal mucin 2) gene, and a decrease in the expression of IGF-2 (insulin-like growth factor 2) and also AvBD2 (avian beta defensin 2) genes. A decreased Enterobacteriaceae counts in the gut of experimental animals showed a positive effect on intestinal microbiota. We confirmed a gut-protecting and an immunostimulatory effect of HS in broiler chickens. /
Other data
OCA16. Annotation of the output in English	The work studied the immunomodulatory and gut-protecting effect of humic substances (HS) in broilers. We noted that HS had a stimulatory effect on the phagocytic activity and the engulfing capacity of phagocytes, however, the level of oxidative burst of phagocytes was not affected. We observed a significant increase of CD4+: CD8+ lymphocyte ratio, an indicator of immune stimulation. HS did not influence the IgA gene expression. In contrast, we observed a significant increase in the expression of MUC-2 (intestinal mucin 2) gene, and a decrease in the expression of IGF-2 (insulin-like growth factor 2) and also AvBD2 (avian beta defensin 2) genes. A decreased Enterobacteriaceae counts in the gut of experimental animals showed a positive effect on intestinal microbiota. We confirmed a gut-protecting and an immunostimulatory effect of HS in broiler chickens.
OCA17. List of maximum 5 most significant citations corresponding to the output	[1] El-Ratel, I.T., El Basuini, M.F.M., Khattab, A.A.A., Mekawy, A.I., Fouda, S.F. Ameliorative impacts of sodium humate on heat-stressed laying Japanese quail (Coturnix coturnix Japonica), (2023) Journal of Animal Physiology and Animal Nutrition, 107 (2), pp. 712-722. [2] Xu, P., Dong, S., Luo, X., Wei, B., Zhang, C., Ji, X., Zhang, J., Zhu, X., Meng, G., Jia, B., Zhang, J. Humic acids alleviate aflatoxin B1-induced hepatic injury by reprogramming gut microbiota and absorbing toxin, (2023) Ecotoxicology and Environmental Safety, 259, art. no. 115051 [3] Elnesr, S.S., Abdel-Razik, A.H., Abdelsalam, A.M., Nabil, T.M., Elwan, H.A.M. Effect of probiotics and humate substances on blood parameters, intestinal development and immune organs of growing quail, (2023) Animal Biotechnology, 34 (8), pp. 3647-3657. [4] Akaichi, A., Jebali, A., Benlarbi, M., Mahjoub, T., Kaboudi, K., Chaouacha-Chekir, R.B., Haouas, Z., Boudhrioua, N. Effects of humic acid and organic acids supplements on performance, meat quality, leukocyte count, and histopathological changes in spleen and liver of broiler chickens, (2022) Research in Veterinary Science, 150, pp. 179-188. [5] HESAMI, Yaser - ESMAIELZADEH, Ladan - KARIMI-TORSHIZI, Mohammad Amir - SEIDAVI, Alireza - VLCKOVA, Radoslava. Effect of diets containing earthworm powder and vermihumus on egg production, hatchability, blood parameters and immunity of Japanese breeder quails. In JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION. ISSN 0931-2439, 2021, vol. 105, no. 2, pp. 316-325., Registrované v: WoS
OCA18. Characteristics of the output's impact on socio-economic practice	The results of the output confirm the positive effect of humic substances on the immune response and thus on the health status of broilers, which will contribute to the introduction of the use of humic substances in broiler fattening in practice. As humic substances have strong detoxifying properties and the leonardite used is also a rich source of minerals, implementation will have a direct impact on the quality of poultry meat and consequently on the health of consumers.
OCA19. Characteristics of the output and related activities' impact on the educational process	The output brings new knowledge about the immune response of poultry after the application of humic substances. As this area is still little studied, this knowledge will be used for further education of students in the 3rd level of university studies in the Immunology program.