| Basic data |
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| OCA1. Surname awarded to the assessed person | Tkáčiková |
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| OCA2. Name awarded to the assessed person | Ľudmila |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/5991 |
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| OCA5. Area of assessment | study programme microbiology and immunology · III. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2024 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 1172418 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://link.springer.com/article/10.1007/s11259-023-10237-4 |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://arl4.library.sk/arl-uvlk/sk/detail-uvlk_un_cat-0043762-Immunomodulatory-effect-of-probiotic-exopolysaccharides-in-a-porcine-in-vitro-coculture-model-mimic/?disprec=1&iset=16 |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | KIŠŠOVÁ, Zuzana - MUDROŇOVÁ, Dagmar - LINK, Róbert - TKÁČIKOVÁ, Ľudmila. Immunomodulatory effect of probiotic exopolysaccharides in a porcine in vitro co-culture model mimicking the intestinal environment on ETEC infection. In: Veterinary Research Communications. 2024. ISSN 0165-7380, Vol. 48, no. 2 (2024), p. 705-724.
(2022: 2.200 - IF, 60 - H-index, 2.300 - IF 5y, 0.526 - SJR, Q1 - SJR Best Q, Q2 - JCR Best Q) |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://link.springer.com/article/10.1007/s11259-023-10237-4 |
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| OCA14. Characteristics of the author's contribution | The author is the bearer of the main ideas and co-creator of the output. At the same time, it is also the holder of funds for the VEGA project from which the output was implemented. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | This study evaluates the immunomodulatory effect of EPS-L26 isolated from the probiotic strain Lactobacillus (Limosilactobacillus) reuteri L26 Biocenol™ in a model of enterotoxigenic E. coli (ETEC) infection by establishing monocultures consisting of the IPEC-J2 cell line or monocyte-derived dendritic cells (moDCs), and by creating a 3D model of cell co-cultures formed from IPEC-J2 and moDCs. The immunomodulatory and immunoprotective potential of the EPS-L26 used was confirmed in monocultures in the experimental group of pretreated cells as well as in a co-culture model on directly treated IPEC-J2 cells cultured on embedded chambers (apical compartment) and also on indirectly treated moDCs cultured in the lower chamber (basolateral compartment), However, in the co-culture model, the expression of inflammasome cytokines at the mRNA level was attenuated compared to monocultures. Pretreatment of cells on the embedded chambers showed immunoprotective properties of EPS-L26, which were reflected by reduced mRNA levels in both cell lines compared to the ETEC challenge. Our results suggest intercellular communication via humoral signals originating from IPEC-J2 cells by affecting gene expression of indirectly treated moDCs located in the basolateral compartment. |
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| Other data |
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| OCA16. Annotation of the output in English | The aim of this study was to evaluate the immunomodulatory effect of EPS-L26 isolated from the probiotic strain Lactobacillus (Limosilactobacillus) reuteri L26 Biocenol™, in a model of infection with an enterotoxigenic E. coli (ETEC) by establishing monocultures consisting of the IPEC-J2 cell line or monocyte-derived dendritic cells (moDCs) and creating a 3D model of cell co-cultures established with IPEC-J2 cells and moDCs. The immunomodulatory and immunoprotective potential of used EPS-L26 was confirmed in monocultures in an experimental group of pretreated cells, where our study showed that pretreatment of cells with EPS-L26 and subsequent exposure to infection resulted in significantly down-regulated mRNA levels of genes encoding inflammatory cytokines compared to ETEC challenge in single cell cultures (in IPEC-J2, decreased mRNA levels for TNF-α, IL-6, IL-1β, IL-12p35; in moDCs, decreased mRNA levels for IL-1β). Similar to monocultures, we also demonstrated the immunostimulatory potential of the ETEC strain in the co-culture model on directly treated IPEC-J2 cells cultivated on insert chambers (apical compartment) and also on indirectly treated moDCs cultivated in the lower chamber (basolateral compartment), however in the co-culture model the expression of inflammatory cytokines was attenuated at the mRNA level compared to monocultures. Pretreatment of the cells on the insert chambers pointed to the immunoprotective properties of EPS-L26, manifested by decreased mRNA levels in both cell lines compared to ETEC challenge (in IPEC-J2 decreased mRNA levels for IL-12p35; in moDCs decreased mRNA levels for IL-1β, IL-6). Our results suggest intercellular communication via humoral signals derived from IPEC-J2 cells by influencing the gene expression of indirectly treated moDC cells located in the basolateral compartment. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | SONG, M. et al. Research progress on the regulatory effects of probiotics and their metabolites on intestinal homeostasis in piglets;. In Zhejiang Daxue Xuebao (Nongye yu Shengming Kexue Ban)/Journal of the Zhejiang University - Agriculture and Life Science [online]. 2024, roč. 50, č. 4, s. 495 – 505. Dostupné na: doi:10.3785/j.issn.1008-9209.2023.11.151
MATSUMOTO, K. et al. Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses. In Bioscience of Microbiota, Food and Health [online]. 2024, roč. 43, č. 4, s. 342 – 351. Dostupné na: doi:10.12938/BMFH.2024-0046 |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The result provides new insights into the possibility of using a 3D cell model to study the effect of exopolysaccharides produced by probiotic bacteria Lactobacillus reuteri in influencing gastrointestinal tract (GIT) inflammation induced by ETEC in pigs. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The output will be used to transmit knowledge in immunology, the study of the immune response in pigs\' gastrointestinal tracts (GIT). |
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