| Basic data |
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| OCA1. Surname awarded to the assessed person | Tkáčiková |
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| OCA2. Name awarded to the assessed person | Ľudmila |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/5991 |
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| OCA5. Area of assessment | study programme microbiology and immunology · III. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2022 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 1004325 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioFormChildE13U66&sid=3695A652D9372D3799417DAF52A9&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://arl4.library.sk/arl-uvlk/sk/detail-uvlk_un_cat-0041026-Immunomodulatory-effect-of-Lactobacillus-reuteri-Limosilactobacillus-reuteri-and-its-exopolysaccha/?disprec=1&iset=15 |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | KIŠŠOVÁ, Zuzana - TKÁČIKOVÁ, Ľudmila - MUDROŇOVÁ, Dagmar - BHIDE, Mangesh Ramesh. Immunomodulatory effect of Lactobacillus reuteri (Limosilactobacillus reuteri) and its exopolysaccharides investigated on epithelial cell line IPEC-J2 challenged with Salmonella Typhimurium. In: Life. 2022. ISSN 2075-1729, Vol. 12, no. 12 (2022), art. no. 1955, p. [1-13]
(2022: 3.200 - IF, 46 - H-index, 3.200 - IF 5y, 0.634 - SJR, Q2 - SJR Best Q, Q2 - JCR Best Q) |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://www.mdpi.com/2075-1729/12/12/1955 |
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| OCA14. Characteristics of the author's contribution | The author is the bearer of the main ideas and co-creator of the output. At the same time, it is also the holder of funds for the VEGA project from which the output was implemented. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | Probiotic bacteria are known for their ability to modulate the mucosal immune system to the benefit of the host organism. Lactobacilli can produce a variety of extracellular polymers known as exopolysaccharides (EPS), which have a wide range of structures, compositions and biological functions. The aim of this study was to investigate how the probiotic strain Limosilactobacillus reuteri L26 and its exopolysaccharide (EPS) affect intestinal epithelial cells of IPEC-J2 pigs infected with Salmonella Typhimurium.
Analysis of mRNA expression for TNF-alpha, IL-8, IL-6, TGF-beta and TLR4, TLR5 signalling pathways in this study indicated the immunoregulatory function of pretreatment of porcine intestinal epithelial cells IPEC-J2 with a probiotic strain of L. reuteri and its exopolysaccharide components compared to Salmonella infection. |
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| Other data |
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| OCA16. Annotation of the output in English | The gastrointestinal tract is the largest and most complex component of the immune
system. Each component influences the production and regulation of cytokines secreted by intestinal epithelial cells. The aim of this study was to see how the probiotic strain Limosilactobacillus reuteri L26 and its exopolysaccharide (EPS) affect porcine intestinal-epithelial cells IPEC-J2 infected with Salmonella Typhimurium. The results revealed that Salmonella infection up-regulated all studied pro-inflammatory cytokines such as TNF-alpha, IL-8, IL-6 and TLR4, TLR5 signaling pathways, while decreasing the expression of TGF-beta. An immunosuppressive activity was found in EPS-treated wells, since the transcriptional levels of the studied pro-inflammatory cytokines were not increased, and the pretreatment with EPS was even able to attenuate up-regulated pro-inflammatory genes induced by Salmonella infection. However, there was a significant increase in the expression of mRNA levels of IL-8 and TNF-alpha in L26-treated cells, although this up-regulation was suppressed in the case of pretreatment. The immunoregulatory function of L. reuteri was also confirmed by the increased level of mRNA expression for TGF-beta, a known immunosuppressive mediator. The most relevant finding of this ex vivo study was a case of immunity modulation, where the probiotic strain L. reuteri stimulated the innate immune-cell response which displayed both anti- and pro-inflammatory activities, and modulated the expression of TLRs in the IPEC-J2 cell line. Our findings also revealed that the pretreatment of cells with either EPS or live lactobacilli before infection has a suppressive effect on the inflammatory response induced by Salmonella Typhimurium. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | LEE, A.H. et al. Limosilactobacillus reuteri - a probiotic gut commensal with contextual impact on immunity. In Gut Microbes [online]. 2025, roč. 17, č. 1. Dostupné na: doi:10.1080/19490976.2025.2451088
KOVÁCS, D. et al. Effects of Luteolin in an In Vitro Model of Porcine Intestinal Infections. In Animals [online]. 2024, roč. 14, č. 13. Dostupné na: doi:10.3390/ani14131952
MA, W. et al. Molecular identification and probiotic potential characterization of lactic acid bacteria isolated from the pigs with superior immune responses. In Frontiers in Microbiology [online]. 2024, roč. 15. Dostupné na: doi:10.3389/fmicb.2024.1361860
SUNG, M. et al. The Immunomodulatory Effect of β-Glucan Depends on the Composition of the Gut Microbiota. In Foods [online]. 2023, roč. 12, č. 17. Dostupné na: doi:10.3390/foods12173148
WALA, S.J. et al. Superior performance of biofilm versus planktonic Limosilactobacillus reuteri in protection of the intestines and brain in a piglet model of necrotizing enterocolitis. In Scientific Reports [online]. 2023, roč. 13, č. 1. Dostupné na: doi:10.1038/s41598-023-44676-5 |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The output provides new insights into the possibilities of using exopolysaccharides produced by the probiotic bacterium Lactobacillus reuteri to influence inflammation of the gastrointestinal tract (GIT) in pigs caused by Salmonella Typhimurium. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The results will be used to transfer knowledge in immunology - the study of the immune response in the gastrointestinal tract (GIT) of pigs. |
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