| Basic data |
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| OCA1. Surname awarded to the assessed person | Tkáčiková |
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| OCA2. Name awarded to the assessed person | Ľudmila |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/5991 |
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| OCA5. Area of assessment | study programme microbiology and immunology · III. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2016 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 0024018 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://www.crepc.sk/portal?fn=*recview&uid=1957227&pageId=resultform&full=0 |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://arl4.library.sk/arl-uvlk/sk/detail-uvlk_un_cat-0024018-Exopolysaccharides-of-Lactobacillus-reuteri-Their-influence-on-adherence-of-E-coli-to-epithelial-c/?disprec=1&iset=2 |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | KŠONŽEKOVÁ, Petra - BYSTRICKÝ, Peter - VLČKOVÁ, Silvia - PÄTOPRSTÝ, Vladimír - BORSZÉKOVÁ PULZOVÁ, Lucia - MUDROŇOVÁ, Dagmar - KUBAŠKOVÁ, Terézia - CSANK, Tomáš - TKÁČIKOVÁ, Ľudmila. Exopolysaccharides of Lactobacillus reuteri: Their influence on adherence of E. coli to epithelial cells and inflammatory response. In Carbohydrate Polymers. ISSN 0144-8617, 2016, vol. 141, s. 10–19. (2016: 4.811 - IF, 5.130 - IF 5y, 192 - H-index, 1.419 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q). |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://www.sciencedirect.com/science/article/pii/S0144861715012151 |
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| OCA14. Characteristics of the author's contribution | The author is the bearer of the main ideas and co-creator of the output. At the same time, it is also the holder of funds for the VEGA project from which the output was implemented. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | The aim of the study was to characterize exopolysaccharides (EPS) originated from Lactobacillus reuteri strain DSM 17938 (EPS-DSM17938) and L. reuteri strain L26 BiocenolTM (EPS-L26) and evaluate their influ- ence on adherence of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells and proinflammatory gene expression. Both EPS were d-glucan polysaccharides with higher molecular weight (Mw), but differing in spatial conformation and elicited variable cytokine profile. EPS-DSM17938, relatively linear polysaccha- ride with (1 → 4) and (1 → 6) glycosidic linkages, increased IL-1β gene expression (P < 0.05), while EPS-L26, more branched polysaccharide with (1 → 3) and (1 → 6) glycosidic linkages, exerted slight but statistically significant up-regulation of NF-kB, TNF-alpha and IL-6 mRNA (P < 0.05). The most significant finding is that preincubation of IPEC-1 cells with both EPS followed by ETEC infection inhibit ETEC adhesion on IPEC-1 cells (P < 0.01) and ETEC-induced gene expression of proinflammatory cytokine IL-1β and IL-6 (P < 0.01). |
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| Other data |
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| OCA16. Annotation of the output in English | The aim of the study was to characterize exopolysaccharides (EPS) originated from Lactobacillus reuteri strain DSM 17938 (EPS-DSM17938) and L. reuteri strain L26 BiocenolTM (EPS-L26) and evaluate their influence on adherence of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells and proinflammatory gene expression. Both EPS were D-glucan polysaccharides with higher molecular weight (Mw), but differing in spatial conformation and elicited variable cytokine profile. EPS-DSM17938, relatively linear polysaccharide with (1 → 4) and (1 → 6) glycosidic linkages, increased IL-1β gene expression (0.1 mg/mL; P < 0.05), while EPS-L26, more branched polysaccharide with (1 → 3) and (1 → 6) glycosidic linkages, exerted slight but statistically significant up-regulation of NF-kB, TNF-alfa and IL-6 mRNA (P < 0.05). The most significant finding is that preincubation of IPEC-1 cells with both EPS followed by ETEC infection inhibit ETEC adhesion on IPEC-1 cells (P < 0.01) and ETEC-induced gene expression of proinflammatory cytokine IL-1β and IL-6 (P < 0.01). |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | YANG, S. et al. Exopolysaccharides from lactic acid bacteria, as an alternative to antibiotics, on regulation of intestinal health and the immune system. In Animal Nutrition [online]. 2023, roč. 13, s. 78 – 89. Dostupné na: doi:10.1016/j.aninu.2023.02.004
ZHANG, J. et al. Lactic acid bacteria-derived exopolysaccharide: Formation, immunomodulatory ability, health effects, and structure-function relationship. In Microbiological Research [online]. 2023, roč. 274. Dostupné na: doi:10.1016/j.micres.2023.127432
HUANG, R. et al. The multiple benefits of bioactive polysaccharides: From the gut to overall health. In Trends in Food Science and Technology [online]. 2024, roč. 152. Dostupné na: doi:10.1016/j.tifs.2024.104677
MANOHARAN, D. et al. Role of Bacteria-Derived Exopolysaccharides in Inflammatory Bowel Disease with a Special Focus on Cyanobacterial Exopolysaccharides. In Applied Microbiology [online]. 2024, roč. 4, č. 1, s. 250 – 274. Dostupné na: doi:10.3390/applmicrobiol4010017
KAVITAKE, D. et al. Production, purification, and functional characterization of glucan exopolysaccharide produced by Enterococcus hirae strain OL616073 of fermented food origin. In International Journal of Biological Macromolecules [online]. 2024, roč. 259. Dostupné na: doi:10.1016/j.ijbiomac.2023.129105 |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The output provides new insights into the potential use of monocyte-derived dendritic cells in in vitro experiments to influence inflammation in the porcine gastrointestinal tract (GIT). |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The output will be used to transfer knowledge in immunology - the study of the immune response in the gastrointestinal tract (GIT) of pigs. |
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