| Basic data |
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| OCA1. Surname awarded to the assessed person | Tkáčiková |
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| OCA2. Name awarded to the assessed person | Ľudmila |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/5991 |
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| OCA5. Area of assessment | study programme microbiology and immunology · III. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2024 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 1240596 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioFormChildS1LQNG&sid=111DEF23A8694C1DD0D92E2AF267&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://arl4.library.sk/arl-uvlk/sk/vysledky/?field=TITLE&term=Exopolysaccharides+from+Limosilactobacillus+reuteri%3A+their+influence+on+in+vitro+activation+of+porcine+monocyte-derived+dendritic+cells+-+brief+report&search=H%C4%BEada%C5%A5&op=result&zf=&sort=&guide= |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | KIŠŠOVÁ, Zuzana – SCHUSTEROVÁ, Petra – Mudroňová, Dagmar – NOVOTNÝ, Jaroslav – TKÁČIKOVÁ, Ľudmila. Exopolysaccharides from Limosilactobacillus reuteri: their influence on in vitro activation of porcine monocyte-derived dendritic cells - brief report. In Veterinary Research Communications [online]. 2024, roč. 48, č. 5, s. 3315 – 3321. Dostupné na: doi:10.1007/s11259-024-10445-6 |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://link.springer.com/article/10.1007/s11259-024-10445-6 |
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| OCA14. Characteristics of the author's contribution | The author is the bearer of the main ideas and co-creator of the output. At the same time, it is also the holder of funds for the VEGA project from which the output was implemented. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | The aim of this study was to evaluate the immunomodulatory potential of exopolysaccharides produced by Limosilactobacillus reuteri L26 Biocenol™ (EPS-L26) and L. reuteri DSM17938 (EPS-DSM17938) in terms of their effect on the in vitro activation of porcine monocyte-derived dendritic cells (MoDCs). We found , that both EPS used promote MoDC maturation. MoDCs treated with both EPS used significantly increased mRNA levels not only for IL-10 but also for IL-12. These cytokines are known to regulate T-cell kinetics and play a key role in maintaining immune homeostasis. Interestingly, we also found that only EPS-DSM17938 significantly increased gene expression of the pro-inflammatory cytokines IL-1β and IL-6. Nevertheless, it is important to emphasize the need for further studies focusing on cytokine kinetics in DCs, as well as co-culture studies with allogeneic T-lymphocytes. |
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| Other data |
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| OCA16. Annotation of the output in English | The aim of this study was to evaluate the immunomodulatory potential of two α-D-glucans from Limosilactobacillus reuteri L26 Biocenol™ (EPS-L26) and L. reuteri DSM17938 (EPS-DSM17938), with respect to their influence on in vitro activation of porcine dendritic cells (DCs). We used immature DCs differentiated from porcine blood monocytes under in vitro conditions. Based on the surface expression of MHC II and costimulatory CD80/86 molecules, we showed that both used EPSs favour the maturation of monocyte-derived DCs (MoDCs) similarly to the commonly used stimulant tumour necrosis factor α (TNF-α). In contrast to TNF-α stimulation, MoDCs treated with both used EPSs significantly up-regulated the mRNA levels not only for interleukin (IL)-10 (P |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | NGUYEN, H.-T. et al. Advances in Microbial Exopolysaccharides: Present and Future Applications. In Biomolecules [online]. 2024, roč. 14, č. 9. Dostupné na: doi:10.3390/biom14091162 |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The output provides new insights into the potential use of exopolysaccharides produced by probiotic strains of Limosilactobacillus reuteri in modulating the immune response in the gastrointestinal tract of pigs. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The results will be used to transfer knowledge in immunology - studying the immune response in the gastrointestinal tract (GIT) of pigs. |
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