ŠTRKOLCOVÁ, Gabriela [45 %] - MRAVCOVÁ, Kristína [5 %] - MUCHA, Rastislav [5 %] - MULINGE, Erastus [1 %] - SCHREIBEROVÁ, Andrea [44 %]. Occurrence of Hookworm and the first molecular and morphometric identification of Uncinaria stenocephala in dogs in Central Europe. In Acta Parasitologica. ISSN 1230-2821, 2022, vol. 67, no. 2, p. 764-772. (2021: 1.534 - IF, 1.427 - IF 5y, 37 - H-index, 0.439 - SJR, Q3 - SJR Best Q, Q2 - JCR Best Q). Dostupné na internete: .

Basic data
OCA1. Surname awarded to the assessed person	Štrkolcová
OCA2. Name awarded to the assessed person	Gabriela
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/26622
OCA5. Area of assessment	study programme veterinary nurse · I. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2022
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	10.1007/s11686-021-00509-x
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildE678B&sid=A1A5DD38B8522D3353A4CF0555&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://pubmed.ncbi.nlm.nih.gov/35067865/
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ŠTRKOLCOVÁ, Gabriela [45 %] - MRAVCOVÁ, Kristína [5 %] - MUCHA, Rastislav [5 %] - MULINGE, Erastus [1 %] - SCHREIBEROVÁ, Andrea [44 %]. Occurrence of Hookworm and the first molecular and morphometric identification of Uncinaria stenocephala in dogs in Central Europe. In Acta Parasitologica. ISSN 1230-2821, 2022, vol. 67, no. 2, p. 764-772. (2021: 1.534 - IF, 1.427 - IF 5y, 37 - H-index, 0.439 - SJR, Q3 - SJR Best Q, Q2 - JCR Best Q). Dostupné na internete: .
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://link.springer.com/article/10.1007/s11686-021-00509-x
OCA14. Characteristics of the author's contribution	First-author; the bearer of ideas in the article.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The aim of this study was to develop a two-step strategy based on morphology and molecular analysis for identifying the genus and species of eggs and larvae from the Ancylostomatidae family in dogs, which were kept under various living conditions in Slovakia. Fecal samples were collected from 270 dogs, 160 of which were from two different shelters, and 110 from a marginalized Roma community. The samples were processed using the flotation method. Fecal samples that were microscopically positive for hookworm eggs underwent coproculture, and the hatched larvae were morphometrically identified before molecular testing. Molecular assays targeting the 18S ribosomal RNA gene fragment were used to identify hookworm eggs and individual larvae. Species-specific primer sets were also designed for the internal transcribed spacer (ITS 1 region) and the mitochondrial cytochrome c oxidase subunit I (COX1) gene regions. Hookworm eggs were microscopically detected in 9.6% (26/270) of the total fecal samples. The prevalence was higher in the Roma settlement (14.5%, 16/110) compared to the shelters (6.3%, 10/160). PCR and subsequent Sanger sequencing identified Uncinaria stenocephala as the canine hookworm species in all positive samples.
Other data
OCA16. Annotation of the output in English	The aim of this study was to develop a two-step strategy based on morphology and molecular analysis for identifying the genus and species of eggs and larvae from the Ancylostomatidae family in dogs, which were kept under various living conditions in Slovakia. Fecal samples were collected from 270 dogs, 160 of which were from two different shelters, and 110 from a marginalized Roma community. The samples were processed using the flotation method. Fecal samples that were microscopically positive for hookworm eggs underwent coproculture, and the hatched larvae were morphometrically identified before molecular testing. Molecular assays targeting the 18S ribosomal RNA gene fragment were used to identify hookworm eggs and individual larvae. Species-specific primer sets were also designed for the internal transcribed spacer (ITS 1 region) and the mitochondrial cytochrome c oxidase subunit I (COX1) gene regions. Hookworm eggs were microscopically detected in 9.6% (26/270) of the total fecal samples. The prevalence was higher in the Roma settlement (14.5%, 16/110) compared to the shelters (6.3%, 10/160). PCR and subsequent Sanger sequencing identified Uncinaria stenocephala as the canine hookworm species in all positive samples.
OCA17. List of maximum 5 most significant citations corresponding to the output	Echinococcus multilocularis and other zoonotic helminths in red foxes (Vulpes vulpes) from a southern German hotspot for human alveolar echinococcosis / Schneider, Carina [Autor, 14.286%] ; Kratzer, Wolfgang [Autor, 14.286%] ; Binzberger, Andreas [Autor, 14.286%] ; Schlingeloff, Patrycja [Autor, 14.286%] ; Baumann, Sven [Autor, 14.285%] ; Romig, Thomas [Autor, 14.285%] ; Schmidberger, Julian [Autor, 14.286%]. – DOI 10.1186/s13071-023-06026-2. – WOS CC In: Parasites & Vectors [elektronický dokument] . – Londýn (Veľká Británia) : Springer Nature. BioMed Central. – ISSN (online) 1756-3305. – Roč. 16, č. 1 (2023), s. [online] Development of In Vitro Assays with the Canine Hookworm Uncinaria stenocephala and Assessment of Natural Plant Products for Anti-Parasitic Activity / Geisshirt, Heidi A. A. [Autor, 20%] ; Bonde, Charlotte S. S. [Autor, 20%] ; Marcussen, Caroline [Autor, 20%] ; Mejer, Helena [Autor, 20%] ; Williams, Andrew R. R. [Autor, 20%]. – DOI 10.3390/pathogens12040536. – WOS CC In: Pathogens [elektronický dokument] . – Bazilej (Švajčiarsko) : Multidisciplinary Digital Publishing Institute. – ISSN (online) 2076-0817. – Roč. 12, č. 4 (2023), s. [online] The hidden threat: Exploring the parasite burden and feeding habits of invasive raccoon dogs (Nyctereutes procyonoides) in central Europe / Schantz, Anna [Autor, 25%] ; Doerge, Dorian D. [Autor, 25%] ; Peter, Norbert [Autor, 25%] ; Klimpel, Sven [Autor, 25%]. – DOI 10.1016/j.ijppaw.2023.10.004. – WOS CC In: International Journal for Parasitology-Parasites and Wildlife [elektronický dokument] . – Holandsko : Elsevier. – ISSN 2213-2244. – Roč. 22 (2023), s. 155-166 [online] Epidemiological and molecular updates on hookworm species in dogs from southern Italy / Illiano, Sergio [Autor, 10%] ; Ciuca, Lavinia [Autor, 10%] ; Maurelli, Maria Paola [Autor, 10%] ; Pepe, Paola [Autor, 10%] ; Caruso, Valeria [Autor, 10%] ; Bosco, Antonio [Autor, 10%] ; Pennacchio, Saverio [Autor, 10%] ; Amato, Ruggero [Autor, 10%] ; Pompameo, Marina [Autor, 10%] ; Rinaldi, Laura [Autor, 10%]. – DOI 10.1186/s12917-023-03765-3. – WOS CC In: BMC Veterinary Research [elektronický dokument] . – Londýn (Veľká Británia) : BMC. – ISSN (online) 1746-6148. – Roč. 19, č. 1 (2023), s. [online] Is there any change in the prevalence of intestinal or cardiopulmonary parasite infections in companion animals (dogs and cats) in Germany between 2004-2006 and 2015-2017? An assessment of the impact of the first ESCCAP guidelines / Vrhovec, Majda Globokar [Autor, 25%] ; Alnassan, Alaa Aldin [Autor, 25%] ; Pantchev, Nikola [Autor, 25%] ; Bauer, Christian [Autor, 25%]. – DOI 10.1016/j.vetpar.2022.109836. – WOS CC ; SCO ; CCC In: Veterinary parasitology [textový dokument (print)] . – Amsterdam (Holandsko) : Elsevier. – ISSN 0304-4017. – ISSN (online) 1873-2550. – Roč. 312 (2022), art. no. 9836 [tlačená forma]
OCA18. Characteristics of the output's impact on socio-economic practice	The study confirmed that the issue of hookworms in dogs in Slovakia remains a current risk to public health, which requires immediate attention and preventive measures. The high positivity rate detected in dogs indicates that this problem could have serious consequences for public health, especially in areas with a higher concentration of domestic animals. Considering global warming, changing climates, and travel to endemic countries, it is crucial to expand research on the spread of infectious hookworm species, as well as other species from the Ancylostomatidae family, to other parts of Slovakia, particularly the southern and warmer regions. Furthermore, strengthening international cooperation with neighboring countries is essential to confirm the presence and spread of these species, which would allow for the development of more effective preventive strategies to protect public health.
OCA19. Characteristics of the output and related activities' impact on the educational process	The publication is also a suitable reference for PhD students studying in the study programme of parasitic diseases of animals.