V3/ADC FAIXOVÁ, Dominika [25 %], RATVAJ, Marek [25 %], ,CINGEĽOVÁ MARUŠČÁKOVÁ, Ivana[10 %], HRČKOVÁ, Gabriela [5 %], KARAFFOVÁ,Viera [5 %],FAIXOVÁ, Zita [5 %], MUDROŇOVÁ, Dagmar [25 %]. Silybin showed higher cytotoxic, antiproliferative, and anti-inflammatory activities in the CaCo cancer cell line while retaining viability and proliferation in normal intestinal IPEC-1 cells. Life. ISSN 2075-1729,2023, vol. 13, no.2, art. no. 492, p. [1-16]. (2022: 3.2 - IF, 3.200 - IF 5y, 46 - H-index, 0.634 - SJR, Q2 - SJR Best Q, Q2

V3/ADC FAIXOVÁ, Dominika [25 %], RATVAJ, Marek [25 %], ,CINGEĽOVÁ MARUŠČÁKOVÁ, Ivana[10 %], HRČKOVÁ, Gabriela [5 %], KARAFFOVÁ,Viera [5 %],FAIXOVÁ, Zita [5 %], MUDROŇOVÁ, Dagmar [25 %]. Silybin showed higher cytotoxic, antiproliferative, and anti-inflammatory activities in the CaCo cancer cell line while retaining viability and proliferation in normal intestinal IPEC-1 cells. Life. ISSN 2075-1729,2023, vol. 13, no.2, art. no. 492, p. [1-16]. (2022: 3.2 - IF, 3.200 - IF 5y, 46 - H-index, 0.634 - SJR, Q2 - SJR Best Q, Q2 - JCR BestQ). Dostupné na internete

Basic data
OCA1. Surname awarded to the assessed person	Faixová
OCA2. Name awarded to the assessed person	Zita
OCA3. Degrees awarded to the assessed person	Prof. , DVM, PhD, MPH
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6015
OCA5. Area of assessment	study programme veterinary morphology and physiology · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2022
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)
OCA9. Hyperlink to the record in CRPA or CRAA
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://doi.org/10.3390/life13020492
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	V3/ADC FAIXOVÁ, Dominika [25 %], RATVAJ, Marek [25 %], ,CINGEĽOVÁ MARUŠČÁKOVÁ, Ivana[10 %], HRČKOVÁ, Gabriela [5 %], KARAFFOVÁ,Viera [5 %],FAIXOVÁ, Zita [5 %], MUDROŇOVÁ, Dagmar [25 %]. Silybin showed higher cytotoxic, antiproliferative, and anti-inflammatory activities in the CaCo cancer cell line while retaining viability and proliferation in normal intestinal IPEC-1 cells. Life. ISSN 2075-1729,2023, vol. 13, no.2, art. no. 492, p. [1-16]. (2022: 3.2 - IF, 3.200 - IF 5y, 46 - H-index, 0.634 - SJR, Q2 - SJR Best Q, Q2 - JCR BestQ). Dostupné na internete
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://doi.org/10.3390/life13020492
OCA14. Characteristics of the author's contribution	ZF is the author of the article, participated in the writing and finalization of the article. ZF is the principal investigator of one of the grants from which the experiment was funded.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	Milk thistle (Silybum marianum L. Gaernt.) is a traditional medicinal plant. The main potential compound of Silybum marianum is a silymarin complex, which contains mainly flavonoids (silybin, silychristin, silydianin, isosilybin, taxifolin, quercetin, dihydrokaempferol, kaempferol, apigenin, naringin, eriodyctiol, and chrysoeriol) and other compounds in very low concentrations. Silybin is the main component of the silymarin complex and is composed of two stereoisomers, silybin A and silybin B. Silymarin complexes are well known for their proven therapeutic effects. In addition to its antioxidant and hepatoprotective effects, silymarin shows neuro-, nephro- and cardioprotective effects in animal and human trials. Moreover, silymarin has an immunomodulatory effect, which includes immunostimulatory as well as immunosuppressive activities. The anticancer potential of silymarin is well known. The mode of action, including its antiproliferative effect mediated by influencing the cell cycle, suppression of apoptosis, and inhibition of cell-survival kinases, as well as its anti-inflammatory effect mediated by inhibition of inflammatory transcription factors, is also relatively well studied. However, there is a lack of studies comparing the effect of silybin on the same type of healthy and tumor cells, especially intestinal, partly due to the limited availability of nontransformed cell lines from different tissues. We have investigated the effect of different concentrations of silybin on healthy intestinal porcine epithelial cell line-1 (IPEC-1) and the cell line of the human epithelial colorectal adenocarcinoma (CaCo-2). We found that silybin stimulates metabolic viability as well as proliferation in IPEC-1 cells, protects the mitochondrial membrane, and thus exerts a cytoprotective effect, and has only a minimal effect on the gene expression of pro-inflammatory cytokines but significantly increases the expression of anti-inflammatory TGF-β. In contrast, it inhibits metabolic viability in tumor intestinal CaCo-2 cells, has an antiproliferative effect accompanied by increased apoptosis, and significantly reduces the expression of genes for pro-inflammatory interleukins as well as TGF-β.
Other data
OCA16. Annotation of the output in English	The anticancer potential of silymarin is well known, including its anti-inflammatory as well as antiproliferative effect mediated by influencing the cell cycle, suppression of apoptosis, and inhibition of cell-survival kinases. However, less is known about silybin, the main component of the silymarin complex, where studies indicate its dual effect on the proliferation and immune response of various cell types in a dose-dependent manner. Moreover, there is a lack of studies comparing the effect of silybin on the same type of healthy and tumor cells, especially intestinal ones. Therefore, our study aimed to investigate the concentration-dependent effect of silybin on the normal intestinal porcine epithelial cell line-1 (IPEC-1) and the human epithelial colorectal adenocarcinoma cell line (CaCo-2). The metabolic viability, cell cycle, mitochondrial membrane potential, apoptosis, and the relative gene expression for pro- and anti-inflammatory cytokines were monitored in cells treated with silybin. Silybin stimulates metabolic viability as well as proliferation in IPEC-1 cells, protects the mitochondrial membrane, and thus exerts a cytoprotective effect, and has only a minimal effect on the gene expression of pro-inflammatory cytokines but significantly increases the expression of anti-inflammatory TGF-β. In contrast, it inhibits metabolic viability in tumor intestinal CaCo-2 cells, has an antiproliferative effect accompanied by increased apoptosis, and significantly reduces the expression of genes for pro-inflammatory interleukins as well as TGF-β.
OCA17. List of maximum 5 most significant citations corresponding to the output
OCA18. Characteristics of the output's impact on socio-economic practice	The antiproliferative and anti-inflammatory effect of silybin on tumor intestinal cells without a negative effect on healthy cells is a prerequisite for its potential use in the adjuvant therapy of colon cancer; however, further studies are necessary.
OCA19. Characteristics of the output and related activities' impact on the educational process	The results of the study are primarily used in the pedagogical process, solving final theses, but also in scientific research activities.