Nosálová, Natália ; Kešeľaková, Alexandra ; Kello, Martin ; Martinková, Miroslava ; Fabiánová, Dominika ; Bago Pilátová, Martina: Involvement of both extrinsic and intrinsic apoptotic pathways in tridecylpyrrolidine-diol derivative-induced apoptosis in vitro. International journal of molecular sciences 2023, Roč. 24, č. 14

Basic data
OCA1. Surname awarded to the assessed person	Bago Pilátová
OCA2. Name awarded to the assessed person	Martina
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/15102
OCA5. Area of assessment	study programme pharmacy (joint study programme) · I. + II. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2023
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID: 1075338
OCA9. Hyperlink to the record in CRPA or CRAA
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://app.crepc.sk/?fn=detailBiblioForm&sid=CF1246C672FE53AF7A91CFF09697
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	Nosálová, Natália ; Kešeľaková, Alexandra ; Kello, Martin ; Martinková, Miroslava ; Fabiánová, Dominika ; Bago Pilátová, Martina: Involvement of both extrinsic and intrinsic apoptotic pathways in tridecylpyrrolidine-diol derivative-induced apoptosis in vitro. International journal of molecular sciences 2023, Roč. 24, č. 14
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://pmc.ncbi.nlm.nih.gov/articles/PMC10380684/pdf/ijms-24-11696.pdf
OCA14. Characteristics of the author's contribution
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.
Other data
OCA16. Annotation of the output in English	Colorectal cancer remains the third leading cause of cancer-related mortality despite recent improvements in treatment outcomes. This study evaluates the anticancer potential of (2S,3S,4R)-2-tridecylpyrrolidine-3,4-diol hydrochloride in colon cancer cell lines Caco-2 and HCT116. The compound exhibited concentration-dependent antiproliferative effects with low micromolar IC₅₀ values, as determined by MTT and BrdU assays. Further analyses using flow cytometry, qRT-PCR, and Western blot revealed the induction of apoptosis through both intrinsic and extrinsic pathways, including mitochondrial membrane depolarization, caspase activation, and regulation of Bcl-2 family proteins. Additionally, the compound suppressed cancer cell migration in a scratch assay. These findings suggest that tridecylpyrrolidine exerts promising anticancer effects via antiproliferative, pro-apoptotic, and anti-migratory mechanisms, highlighting its potential as a therapeutic agent for colorectal cancer.
OCA17. List of maximum 5 most significant citations corresponding to the output	TUFAIL, Muhammad - JIANG, Can Hua - LI, Ning. Altered metabolism in cancer: insights into energy pathways and therapeutic targets. In Molecular Cancer, 2024, roč. 23, č. 1, 02119; doi.org/10.1186/s12943-024-02119-3; Q1 IF: 27.7
OCA18. Characteristics of the output's impact on socio-economic practice	Colorectal cancer represents a serious societal and economic issue due to its high incidence and mortality rates, particularly in economically developed countries. The findings of this study, which demonstrated promising anticancer activity of (2S,3S,4R)-2-tridecylpyrrolidine-3,4-diol hydrochloride, could significantly contribute to the development of new, more effective, and targeted therapeutic approaches. If the efficacy of this compound is further confirmed in preclinical and clinical studies, it may lead to improved prognosis for patients with colorectal cancer, reduced burden on healthcare systems, and ultimately enhanced quality of life for cancer patients. Furthermore, the potential development of a new drug represents added value for the pharmaceutical industry, innovation, and the transfer of scientific knowledge into practice, thereby supporting economic growth in the field of biomedical research.
OCA19. Characteristics of the output and related activities' impact on the educational process	The results of the study also impact the educational process in undergraduate and postgraduate education in biomedical and pharmaceutical sciences. Through participation in research activities, students can learn to work with modern laboratory techniques such as flow cytometry, qRT-PCR, and Western blot, thereby gaining practical skills and a deeper understanding of the molecular mechanisms of cancer. The study also supports the development of critical thinking and analytical skills in the interpretation of experimental data. Additionally, it creates opportunities for students to engage in multidisciplinary research teams, enhancing their readiness for professional careers in scientific, clinical, and pharmaceutical environments. The research findings can also be incorporated into teaching through lectures focused on modern approaches in oncology research and drug development.