| Basic data |
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| OCA1. Surname awarded to the assessed person | Legáth |
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| OCA2. Name awarded to the assessed person | Jaroslav |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, CSc. |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/2269 |
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| OCA5. Area of assessment | study programme pharmacy (joint study programme) · I. + II. degree |
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| OCA6. Category of the research/artistic/other output | |
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| OCA7. Year of publication of the research/artistic/other output | 2024 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | https://doi.org/10.1038/s41598 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | DYBA, Barbara - RUDOLPHI-SZYDŁO, Elżbieta - KRECZMER, Barbara - BARBASZ, Anna - PETRILLA, Vladimír - PETRILLOVÁ, Monika - LEGÁTH, Jaroslav - BOCIAN, Aleksandra - HUS, Konrad Kamil. Exploring the effects of three-finger toxins from Naja ashei venom on neuronal and immunological cancer cell membranes. In: Scientific Reports. 2024. ISSN 2045-2322, Vol. 14, no. 1 (2024), art. no. 18570, p. [1-12]
(2022: 4.600 - IF, 282 - H-index, 4.900 - IF 5y, 0.973 - SJR, Q1 - SJR Best Q, Q2 - JCR Best Q) |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://www.nature.com/articles/s41598-024-69459-4 |
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| OCA14. Characteristics of the author's contribution | |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | |
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| Other data |
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| OCA16. Annotation of the output in English | Three-finger proteins are the most abundant toxins in the venom of Naja ashei, a snake species from the Elapidae family. This research aimed to describe the effects of varying charges of these proteins, isolated from Naja ashei venom using SEC and IEX chromatography. The study examined how differently charged three-finger toxin fractions interact with and affect neuroblastoma (SK-N-SH) and promyeloblast (HL-60) cells, as well as model Langmuir membranes and liposomes designed to mimic cellular lipid composition. Findings revealed that protein surface charges significantly impact cell survival (MTT assay), membrane damage (lactate dehydrogenase release, malondialdehyde formation), and the structural and electrochemical properties of model membranes (Langmuir membranes and zeta potential for liposomes and cancer cell lines). Results indicated that SK-N-SH cells, characterized by a higher negative charge on their cell membranes, interacted more effectively with positively charged toxins than HL-60 cells. However, the mechanism of these electrostatic interactions is complex. The research demonstrated that electrostatic and mechanical membrane modifications induced by venom proteins can significantly affect cell metabolism. Additionally, the total charge of the membrane, influenced by polar lipid components and phospholipid saturation, plays a decisive role in toxin interaction. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | |
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| OCA18. Characteristics of the output's impact on socio-economic practice | |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | |
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