The use of flow cytometry in the analysis of sows’ colostrum and milk / Novotný, Jaroslav [Autor, 50%] ; Mudroňová, Dagmar [Autor, 25%] ; Krepelková, Zuzana [Autor, 10%] ; Bárdová, Katarína [Autor, 10%] ; D´Alfonso, Alexia [Autor, 5%]. – [recenzované]. – DOI 10.2478/fv-2024-0026. In: Folia veterinaria [textový dokument (print)] [elektronický dokument] : the scientific journal of the University of Veterinary Medicine and Pharmacy in Košice. – Košice (Slovensko) : Univerzita veterinárskeho lekárstva a farmácie v Košiciach. – ISSN 0015-5748. – ISSN (online) 2453-7837. – Roč. 68, č. 3 (2024), s. 44-51 [tlačená forma]

Basic data
OCA1. Surname awarded to the assessed personNovotný
OCA2. Name awarded to the assessed personJaroslav
OCA3. Degrees awarded to the assessed personAssoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staffhttps://www.portalvs.sk/regzam/detail/6072
OCA5. Area of assessmentstudy programme general veterinary medicine · I. + II. degree
OCA6. Category of the research/artistic/other output
OCA7. Year of publication of the research/artistic/other output2024
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)1226599
OCA9. Hyperlink to the record in CRPA or CRAAhttps://app.crepc.sk/?fn=detailBiblioForm&sid=7E4A807172B6E37507706C527484
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputschrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://sciendo-parsed.s3.eu-central-1.amazonaws.com/66ec70957d402026d60a47ec/10.2478_fv-2024-0026.pdf?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=ASIA6AP2G7AKDIUHP5RZ%2F20250821%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20250821T094558Z&X-Amz-Expires=3600&X-Amz-Security-Token=IQoJb3JpZ2luX2VjEKH%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaDGV1LWNlbnRyYWwtMSJHMEUCIQDu1H4eiDAAQbOATlkIOQBrUYQq5qXAtrLrSSBgIJN52wIgYTA08nh5PXMcQb3ca2vHbvL4tNbfHEXIRs9GPHyMhJcqxgUI6v%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FARACGgw5NjMxMzQyODk5NDAiDP2HYdMtxSSgRO6aoSqaBTai99A0PQj8CfQgaL2A86V2IUSm%2FBY%2Fseair031LQStoF%2FC7c%2BWCa0rJcW82W%2Bm17DKigBGe%2F14tdT2RtMBtNPyLvshbPPLcEpeUj2xXWBIpWVzEiBGNCxLxBqwAObQPQY%2F2Cue%2Bv9XifJ3i8wJzSwJPRZKP%2BrA4zVO3Avs%2BuIigwabvoiQs5oz5ysK98qma4ePu%2F3JkfrEYLGtXGyGbi%2BfZLehAagKg8zvZsqf7ifydqTkshXWIJsMyfFl6PwSBIiZD6RIoiLxSWeTEevjhFuNs2FeHf3ZxAKLr2jDeaD%2FJ3KaKD06p%2Fw4t4r6CqLs8AzmlXGuraddJBq28nQ3BxuZCWpvSCG7tM14pcVlec7Jy795n%2FIVs2PLCzrsvIBJQKgtR3WwtmkKjSVsXsEX7GBSsY%2BCQTwmrFCq74QV1JSrU4V5XEbR6Z5bWFyLf0F1EamDfnnMZX43qjNEjxWwVXRqvfkmNQEWuDnkW8KMKckFsfaFkFO257xd8ICgnj3P9DLiX%2FlBG2AaIXgBR3DUKm3u2Vkt4QSFaLvFuB%2BTbiaBkiKDCUlTX6oBvGUt5I%2Bgdb7KLuDkM0dgSuRT4CoBtyeGU8OVmG66Xo%2F9WYTFo0T%2Faf0rfbMaySr06Jz8H%2By33opReSZKYJjE4jRxYDgIfcb%2BBWbRKNJHacbZqnx0euZL8zJM1roScYfWsbnor6Tv0H7WPT4huYO0xyR6AxRk%2Fw%2BdCYRiuhZ3jmjAwadqFiFMpgPkhszOYA1Vw%2BpUwq5vMN1CQb0SVmcylEl2NdAXu%2BT20I9RCeXXobQpskrDDoNjW%2BaQ%2BtDkqTM0lUyfKH3R8AvEWFa8hXsbQRg%2FczBJPs%2Fq92b%2FdaxBoaeLYnLRF5Qz9m2MAHn4nUwzQjDJyZvFBjqxAQe%2FX%2BNoVxf92FEYnipH5BhYkSzTYYL6uFcobd8k6byi%2FLH%2F6VxqWcwgiNBGLg7ToQcde3Dr3FLUo0DEDgsaPHuL%2Bq8NUdTfM81M8NvBkNjn2rE5WIGgYPAm4QWV5%2BswbDpsBKLZXzcodvzvy4ELnRFZt4oIht2djQ0Te0rIZZcL9OF%2BgPG%2FEfzTyek4scfVGSHn6qciG%2Bh49%2Bat6GQ2q1HPkHQ8GTXbbmqs4BmQCgZ76A%3D%3D&X-Amz-Signature=302944e0426d1907587cbab3b8d3a34dccf6b3fa27603b133dafd9ddd1af5f01&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputsThe use of flow cytometry in the analysis of sows’ colostrum and milk / Novotný, Jaroslav [Autor, 50%] ; Mudroňová, Dagmar [Autor, 25%] ; Krepelková, Zuzana [Autor, 10%] ; Bárdová, Katarína [Autor, 10%] ; D´Alfonso, Alexia [Autor, 5%]. – [recenzované]. – DOI 10.2478/fv-2024-0026. In: Folia veterinaria [textový dokument (print)] [elektronický dokument] : the scientific journal of the University of Veterinary Medicine and Pharmacy in Košice. – Košice (Slovensko) : Univerzita veterinárskeho lekárstva a farmácie v Košiciach. – ISSN 0015-5748. – ISSN (online) 2453-7837. – Roč. 68, č. 3 (2024), s. 44-51 [tlačená forma]
OCA12. Type of the output (if the output is not registered in CRPA or CRAA) article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)https://www.researchgate.net/publication/384171930_The_Use_of_Flow_Cytometry_in_the_Analysis_of_Sows'_Colostrum_and_Milk
OCA14. Characteristics of the author's contributionConceptualization, Methodology, Investigation, Funding acquisition, Writing – review & editing.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.The flow cytometry method is used in many fields, not only scientific, but also clinical. In science, flow cytometry is used in immunology, molecular biology, microbiology or plant biology. In medicine, has its use, in the diagnosis of tumours, in reproductive and prenatal diagnosis, in transplants or in haematology. In our experimental work, we used this method to analyse colostrum and milk of sows. The aim of this study was to determine the number of somatic cells, the number of leukocytes and the number of T-lymphocytes. Colostrum samples were collected within 24 hours of delivery and then milk samples were collected at weekly intervals during 3 weeks. Statistical analysis revealed significant differences in the number of leukocytes (CD45+) in colostrum and milk. The most significant difference was noted between colostrum and milk in weeks 2 and 3 of the experiment (P < 0.0001), with the highest values found in colostrum. On the other hand, we found that the levels of helper T-cells (CD4+) and cytotoxic T-cells (CD8+) gradually increased over time (P > 0.05), with the highest values observed in the third week after farrowing. We can conclude that flow cytometry can be successfully used for the examination of somatic cells in sows’ milk.
Other data
OCA16. Annotation of the output in English
OCA17. List of maximum 5 most significant citations corresponding to the output
OCA18. Characteristics of the output's impact on socio-economic practiceBased on the provided abstract, the impact of the output on socio-economic practice can be characterized as follows: Improvement of milk quality and piglet health: Identifying the number of somatic cells, leukocytes, and T-lymphocytes in colostrum and milk allows for better assessment of milk immunological quality and the health status of piglets, which can lead to more effective disease prevention in the herd. Support for veterinary practice and herd management: The results provide veterinarians and farmers with concrete data to monitor the immune status of pigs, optimize nutrition and hygiene measures, thereby reducing disease incidence and improving productivity. Economic benefit: Better health and immunity of pigs, along with improved colostrum and milk quality, can reduce mortality and the need for treatment, leading to higher farm efficiency and reduced economic losses in large-scale pig production.
OCA19. Characteristics of the output and related activities' impact on the educational processThe publication is suitable study literature for students of the general veterinary medicine and food hygiene study program.

Last updated: 28.10.2026

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