Solárová Zuzana, Kello Martin, Hamuľaková Slávka, Mirossay Ladislav, Solár, Peter: Anti-cancer effect of tacrine-coumarin derivatives on diverse human and mouse cancer cell lines, Acta Chimica Slovenica, ADC - Vedecké práce v zahraničných karentovaných časopisoch IF 1,263, Q2-SJR, citované 0x

Basic data
OCA1. Surname awarded to the assessed person	Solárová
OCA2. Name awarded to the assessed person	Zuzana
OCA3. Degrees awarded to the assessed person	Assoc. prof. , M.D., PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/15120?mode=full
OCA5. Area of assessment	study programme pharmacy (joint study programme) · I. + II. degree
OCA6. Category of the research/artistic/other output
OCA7. Year of publication of the research/artistic/other output	2021
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildGO9M4&sid=D79AF36B738BACD5C0416029&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	Solárová Zuzana, Kello Martin, Hamuľaková Slávka, Mirossay Ladislav, Solár, Peter: Anti-cancer effect of tacrine-coumarin derivatives on diverse human and mouse cancer cell lines, Acta Chimica Slovenica, ADC - Vedecké práce v zahraničných karentovaných časopisoch IF 1,263, Q2-SJR, citované 0x
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)
OCA14. Characteristics of the author's contribution	SOLÁROVÁ, Zuzana (75%) - KELLO, Martin (10%) - SOLÁR, Peter (15%)
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	Phytoalexins are substances with antimicrobial properties that plants require after attack by microorganisms, especially phytopathogenic fungi and viruses. They are also currently being investigated for their antitumor effect. Our aim was to study homobrassinin and thiazino[6,5-b]indole for the stimulation of apoptosis in human ovarian adenocarcinoma A2780 and A2780cis cells using flow cytometry - Annexin V/PI analysis, caspase activity.
Other data
OCA16. Annotation of the output in English	Phytoalexins are substances with antimicrobial properties produced by plants after being attacked by microorganisms, especially phytopathogenic fungi and viruses. They are also currently being studied for their antitumor effect. We aimed to study the apoptosis-stimulating effect of homobrassinin and thiazino[6,5-b]indol in human ovarian adenocarcinoma A2780 and A2780cis cells via flow cytometric analysis of annexin V/PI, caspase 3 and 9 activity, cytochrome C release, and smac-diablo accumulation. Using the western blot technique, we also monitored the effect of both indoles on the response of heat shock proteins in these cells. Thiazino[6,5-b]indol showed more pronounced sensitizing and/or pro-apoptotic effect compared to homobrassinin accompanied by increased smac-diablo accumulation at earlier time intervals and pronounced externalization of phosphatidylserine at 72 h in A2780cis compared to A2780 cells. The apoptosis stimulating effect of thiazino[6,5-b]indol in A2780cis cells was associated with significant irreversible downregulation of HSP70 and HSP90 and partly with a decrease of HSP40.
OCA17. List of maximum 5 most significant citations corresponding to the output
OCA18. Characteristics of the output's impact on socio-economic practice	The work is focused on the possible antitumor effect of natural substances, as well as the search for new targets of anticancer drugs, which we have been dealing with at our institute for a long time. Numerous bioactive phytochemicals have been studied for their antitumor potential. This article focuses on the antiproliferative effects of selected indoles. Indoles, as substances of natural origin, affect a new target - heat shock proteins, which are highly expressed in cancer cells.
OCA19. Characteristics of the output and related activities' impact on the educational process	The anticancer drugs are part of lectures for medical students. For PhD students, the potential antitumor effect of natural substances is an important part of their studies. This work can help them understand the new targets for many of anticancer drugs.