Sulfation of furcellaran and its effect on hemocompatibility in vitro / Štěpánková, Kateřina [Autor, 20%] ; Ozaltin, Kadir [Autor, 15%] ; Gorejová, Radka [Autor, 15%] ; Doudová, Hana [Autor, 5%] ; Domincová Bergerová, Eva [Autor, 5%] ; Maskaľová, Iveta [Autor, 5%] ; Stupavská, Monika [Autor, 5%] ; Sťahel, Pavel [Autor, 5%] ; Trunec, David [Autor, 5%] ; Pelková, Jana [Autor, 5%] ; Mozetic, Miran [Autor, 5%] ; Lehocký, Marián [Autor, 10%]. – [recenzované]. – DOI 10.1016/j.ijbiomac.2023.128840. – CCC ; SCO ; WOS CC. In: International Journal of Biological Macromolecules [textový dokument (print)] [elektronický dokument] : Structure, Function and Interactions. – Oxford (Veľká Británia) : Elsevier. – ISSN 0141-8130. – ISSN (online) 1879-0003. – Roč. 254, č. 1 (2024), art. no. 128840, s. [1-12] [tlačená forma] [online]

Basic data
OCA1. Surname awarded to the assessed person	Maskaľová
OCA2. Name awarded to the assessed person	Iveta
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6064
OCA5. Area of assessment	study programme nutrition of animals and dietetics · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2024
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID: 1128796
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=B13AEFF39C4E73C41E8DF5817B0B
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	Sulfation of furcellaran and its effect on hemocompatibility in vitro / Štěpánková, Kateřina [Autor, 20%] ; Ozaltin, Kadir [Autor, 15%] ; Gorejová, Radka [Autor, 15%] ; Doudová, Hana [Autor, 5%] ; Domincová Bergerová, Eva [Autor, 5%] ; Maskaľová, Iveta [Autor, 5%] ; Stupavská, Monika [Autor, 5%] ; Sťahel, Pavel [Autor, 5%] ; Trunec, David [Autor, 5%] ; Pelková, Jana [Autor, 5%] ; Mozetic, Miran [Autor, 5%] ; Lehocký, Marián [Autor, 10%]. – [recenzované]. – DOI 10.1016/j.ijbiomac.2023.128840. – CCC ; SCO ; WOS CC. In: International Journal of Biological Macromolecules [textový dokument (print)] [elektronický dokument] : Structure, Function and Interactions. – Oxford (Veľká Británia) : Elsevier. – ISSN 0141-8130. – ISSN (online) 1879-0003. – Roč. 254, č. 1 (2024), art. no. 128840, s. [1-12] [tlačená forma] [online]
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.sciencedirect.com/science/article/pii/S0141813023057392?casa_token=qWR_RZE4mncAAAAA:qdIR9dn2N6xaSqfckO76G3D4bEKWAg_Gl_8kLoUgvUwp537uEn5_3G2r2RRgwkpG7NR1pE0BnR8
OCA14. Characteristics of the author's contribution	Co-author and collaborator
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The scientific article analyzes sulfate derivatives and the essential role of sulfate esters in providing anticoagulant activity. These findings support their key role in improving hemocompatibility and provide valuable insights for the comparative evaluation of effective sulfation approaches.
Other data
OCA16. Annotation of the output in English	In this study, furcellaran (FUR) obtained from Furcellaria lumbricalis was firstly employed for sulfation via various methods, including SO3-pyridine (SO3∙Py) complex in different aprotic solvents, chlorosulfonic acid and sulfuric acid with a “coupling” reagent N,N′-Dicyclohexylcarbodiimide. Structural characterization through FT-IR, GPC, XPS and elemental analyses confirmed the successful synthesis of 6-O-sulfated FUR derivates characterized by varying degrees of sulfation (DS) ranging from 0.15 to 0.91 and molecular weight (Mw) spanning from12.5 kDa to 2.7 kDa. In vitro clotting assays, partial thromboplastin time (aPTT), thrombin time (TT), and prothrombin time (PT) underscored the essential role of sulfate esters in conferring anticoagulant activity whereas FUR prepared via chlorosulfonic acid with DS of 0.91 reached 311.4 s in aPPT showing almost 4-fold higher anticoagulant activity than native FUR at the concentration 2 mg/mL. MTT test showed all tested samples decreased cell viability in a dose dependent manner while all of them are non-cytotoxic up to the concentration of 0.1 mg/mL. Furthermore, sulfated derivates deposited onto polyethylene terephthalate surface presented substantial decrease in platelet adhesion, as well as absence of the most activated platelet stages. These findings support the pivotal role of O-6 FUR sulfates in enhancing hemocompatibility and provide valuable insights for a comparative assessment of effective sulfating approaches.
OCA17. List of maximum 5 most significant citations corresponding to the output	Zhu, C., Li, Y., Deng, Q., Guo, R., Huang, Y., Guo, W., ... & Sun, Z. (2026). Achieving superior therapeutic effects on methicillin‐resistant Staphylococcus aureus infection via simple sulfonation process for preparing zwitterionic photosensitizer. Interdisciplinary Medicine, e70095 Petcharat, T., Chaijan, M., Indriani, S., Karnjanapratum, S., Nirmal, N., Singh, J., ... & Nalinanon, S. (2025). Physicochemical, Sensorial and Calcium Bioavailability of Jelly Prepared Using Fish Gelatin in Combination with Furcellaran and Calcium L-Threonate. Gels, 12(1), 26. Nowak, E., Żak, J., Janik, M., Cholewa-Wójcik, A., Juszczak, L., Szuwarzyński, M., ... & Jamróz, E. (2025). Double-Layer Films Based on Furcellaran/Chitosan Complex—Structural and Functional Characteristics of Packaging Materials. International Journal of Molecular Sciences, 26(20), 10049. Premarathna, A. D., Sooäär, A., Humayun, S., Ahmed, T. A., Rjabovs, V., Critchley, A. T., ... & Tuvikene, R. (2025). Structural characterization and in vitro bioactivity of Furcellaria lumbricalis polysaccharides: Implications for skin, immune, and gut health applications. International Journal of Biological Macromolecules, 147696. Humayun, S., Rjabovs, V., Justine, E. E., Darko, C. N. S., Howlader, M. M., Reile, I., ... & Tuvikene, R. (2025). Immunomodulatory activity of red algal galactans and their partially depolymerized derivatives in RAW264. 7 macrophages. Carbohydrate Polymers, 347, 122741.
OCA18. Characteristics of the output's impact on socio-economic practice	Based on the obtained results, the findings support the key role of sulfate esters in improving hemocompatibility and provide valuable insights for the comparative evaluation of effective sulfation approaches.
OCA19. Characteristics of the output and related activities' impact on the educational process	The publication suitably complements the study literature in undergraduate and postgraduate education and provides insight into the use of sulfate esters in providing anticoagulant activity.