MASKAĽOVÁ, Iveta - VAJDA, Vladimír - BUJŇÁK, Lukáš. 2,6-Diaminopimelic acid as a biological marker of rumen synthesis and fermentation capacities in the transition period and early lactation of dairy cows [2,6 Diaminopelová kyselina ako biologický marker bachorovej syntézy a fermentačnej kapacity v prechodnom období a skorej laktácie dojníc]. In: Acta veterinaria Brno. 2014. ISSN 0001-7213, Vol. 83, č. 4 (2014), s. 355-361. (2014: 0.469 - IF, 36 - H-index, 0.047 - IF 5y, 0.297 - SJR, Q3 - SJR Best Q, Q3

MASKAĽOVÁ, Iveta - VAJDA, Vladimír - BUJŇÁK, Lukáš. 2,6-Diaminopimelic acid as a biological marker of rumen synthesis and fermentation capacities in the transition period and early lactation of dairy cows [2,6 Diaminopelová kyselina ako biologický marker bachorovej syntézy a fermentačnej kapacity v prechodnom období a skorej laktácie dojníc]. In: Acta veterinaria Brno. 2014. ISSN 0001-7213, Vol. 83, č. 4 (2014), s. 355-361. (2014: 0.469 - IF, 36 - H-index, 0.047 - IF 5y, 0.297 - SJR, Q3 - SJR Best Q, Q3 - JCR Best Q)

Basic data
OCA1. Surname awarded to the assessed person	Maskaľová
OCA2. Name awarded to the assessed person	Iveta
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6064
OCA5. Area of assessment	study programme nutrition of animals and dietetics · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2014
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID: 635527
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=B2A3331078C3993D45E7E9200C
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	MASKAĽOVÁ, Iveta - VAJDA, Vladimír - BUJŇÁK, Lukáš. 2,6-Diaminopimelic acid as a biological marker of rumen synthesis and fermentation capacities in the transition period and early lactation of dairy cows [2,6 Diaminopelová kyselina ako biologický marker bachorovej syntézy a fermentačnej kapacity v prechodnom období a skorej laktácie dojníc]. In: Acta veterinaria Brno. 2014. ISSN 0001-7213, Vol. 83, č. 4 (2014), s. 355-361. (2014: 0.469 - IF, 36 - H-index, 0.047 - IF 5y, 0.297 - SJR, Q3 - SJR Best Q, Q3 - JCR Best Q)
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://actavet.vfu.cz/83/4/355/
OCA14. Characteristics of the author's contribution	The author is the carrier of ideas and the main researcher in the field.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The scientific article evaluates the effect of different nutrient concentrations and availability in the feed ration on rumen fermentation and synthetic capacity in dairy cows in the transition period and phase 1 of lactation. The obtained results confirmed the use of 2,6-diaminopimelic acid as a promising marker for monitoring and evaluating rumen synthetic function and bacterial biomass production.
Other data
OCA16. Annotation of the output in English	The experiment was conducted to evaluate the effect of different types of available nutrients of total mix rations on fermentation and synthesis capacities of the rumen in Holstein dairy cows divided into groups in the transition period (± 3 weeks after parturition) and early lactation (60–80 days in milk). The diets were fed as a total mixed ration. Results obtained with feeding Diet C (high concentration of available nutrients) to dairy cows confirmed a significant increase in 2,6-diaminopimelic acid from the initial mean 2.2 μg/ml at 21 days before calving to 6.2 μg/ml (P < 0.01) at 21 days after calving or 7.6 μg/ml (P < 0.01) at early lactation, and a parallel significant increase in the concentration of volatile fatty acids (P < 0.01). The concentration of propionic acid increased from the initial mean 25.3 to 39.3 mmol/l (P < 0.01) in the transition period, and to 43.1 mmol/l at early lactation (P < 0.01). All indicators of rumen fermentation and synthesis determined in dairy cows with Diet B (low concentration of available nutrients) of the total mix ration according to productive phases were not significant. High regression coefficients of examined indicators of 2,6-diaminopimelic acid to volatile fatty acid (r = 0.813), propionic acid (r = 0.780) and acetic acid (r = 0.635) indicate direct correlation between fermentation and synthesis activities of the rumen. Our results show that 2,6-diaminopimelic acid appears as a perspective marker for the monitoring and evaluation of the synthesis function of the rumen and production of bacterial biomass under breeding conditions.
OCA17. List of maximum 5 most significant citations corresponding to the output	Chandra Roy, A., Wang, Y., Zhang, H., Roy, S., Dai, H., Chang, G., & Shen, X. (2018). Sodium butyrate mitigates iE-DAP induced inflammation caused by high-concentrate feeding in liver of dairy goats. Journal of agricultural and food chemistry, 66(34), 8999-9009. Orellana, C., Parraguez, V. H., Arana, W., Escanilla, J., Zavaleta, C., & Castellaro, G. (2019). Use of fecal indices as a non-invasive tool for nutritional evaluation in extensive-grazing sheep. Animals, 10(1), 46. Vigh, A., Criste, A., Gragnic, K., Moquet, L., & Gerard, C. (2023). Ruminal solubility and bioavailability of inorganic trace mineral sources and effects on fermentation activity measured in vitro. Agriculture, 13(4), 879. Hanušovský, O., Šimko, M., Rolinec, M., Gálik, B., Kapusniaková, M., Drotárová, S., ... & Juráček, M. (2025). Effect of TMR Physical Structure and Ruminal pH Environment on Production and Milk Quality. Dairy, 6(5), 51. Vigh, A., Criste, A., Gragnic, K., Moquet, L., & Gerard, C. (2023). Ruminal solubility and bioavailability of inorganic trace mineral sources and effects on fermentation activity measured in vitro. Agriculture, 13(4), 879.
OCA18. Characteristics of the output's impact on socio-economic practice	Analysis and evaluation of 2,6-diaminopimelic acid appears to be a promising marker for monitoring and evaluating rumen synthetic function and bacterial biomass production under farm conditions.
OCA19. Characteristics of the output and related activities' impact on the educational process	The publication suitably complements the study literature in undergraduate and postgraduate education in the evaluation of rumen metabolism; it brings a new perspective on the evaluation of microbial protein synthesis and the synthetic capacity of the rumen at the farm level.