| OCA16. Annotation of the output in English | Amyloidoses represent a group of pathological conditions characterized by amyloid fibrils accumulating in the form of deposits in intra- or extracellular space, leading to tissue damage. The lysozyme from hen egg-white (HEWL) is often used as a universal model protein to study the anti-amyloid effects of small molecules. The in vitro anti-amyloid activity and mutual interactions of green tea leaf constituents: (−)-epigallocatechin gallate (EGCG), (−)-epicatechin (EC), gallic acid (GA), caffeine (CF) and their equimolar mixtures were studied. The inhibition of HEWL amyloid aggregation was monitored by a Thioflavin T fluorescence assay and atomic force microscopy (AFM). The interactions of the analyzed molecules with HEWL were interpreted by ATR-FTIR and protein-small ligand docking studies. EGCG was the only substance efficiently inhibiting amyloid formation (IC50 ∼193 μM), slowing the aggregation process, reducing the number of fibrils and partially stabilizing the secondary structure of HEWL. Compared to EGCG alone, EGCG-containing mixtures displayed lower overall anti-amyloid efficacy. The decrease in efficiency results from (a) the spatial interference of GA, CF and EC with EGCG while binding to HEWL, (b) the propensity of CF to form a less active adduct with EGCG, which participates in interactions with HEWL in parallel with pure EGCG. This study confirms the importance of interaction studies, revealing the possible antagonistic behavior of molecules when combined. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | 2025 [01/20] / GBR - Kung, Yu Hsuan - Chang, Chia Yu - Lai, You Ren - Li, Jia Xun - How, Su Chun. Examining the inhibitory potency of metal polyphenolic network–coated silver nanoparticles against amyloid fibrillogenesis of lysozyme [elektronický dokument]. DOI 10.1016/j.saa.2024.125375 In: Spectrochimica Acta: Part A: Molecular and Biomolecular Spectroscopy. Oxford: Elsevier. Pergamon Press, 2025, č. 327, art. no. 125375, s. [1-15] [tlačená forma] [online]. ISSN 1386-1425. ISSN (online) 1873-3557. - SCO ; CCC ; WOS CC ; SCIE, s. [1 - 15]
2024 [01/20] / CHE - Gil, María Victoria - Fernández-Rivera, Nuria - Gutiérrez-Díaz, Gloria - Parrón-Ballesteros, Jorge - Pastor-Vargas, Carlos - Betancor, Diana - Nieto, Carlos - Cintas, Pedro. Antioxidant Activity and Hypoallergenicity of Egg Protein Matrices Containing Polyphenols from Citrus Waste [elektronický dokument]. DOI 10.3390/antiox13101154 In: Antioxidants. Bazilej: Multidisciplinary Digital Publishing Institute, 2024, Roč. 13, č. 10, art. no. 1154, s. [1-33] [online]. ISSN (online) 2076-3921. - SCO ; OA ; WOS CC ; SCIE, s. [1 - 33]
2024 [01/20] / NLD - Sun, Yidan - Yan, Chaoqun - Liang, Tingting - Li, Wen - Pei, Minfan - Zhang, Linyan - Li, Qingshan - Liang, Taigang. Comparative study on interactions of phillyrin and phillygenol with lysozyme: Spectroscopy, differential scanning calorimetry and molecular modeling approaches [elektronický dokument]. DOI 10.1016/j.molliq.2024.124571 In: Journal of Molecular Liquids. Amsterdam: Elsevier, 2024, č. 401, art. no. 124571, s. [1-14] [tlačená forma] [online]. ISSN 0167-7322. ISSN (online) 1873-3166. - SCO, s. [1 - 14]
2024 [01/20] / NLD - Hasan, Md Rashed - Haque, Mohammad Majedul - Hoque, Md Amirul - Sultana, Shahin - Rahman, Mohammad Mahbubur - Ali Shaikh, Md Aftab - Sarker, Md Khabir Uddin. Antioxidant activity study and GC-MS profiling of Camellia sinensis Linn [elektronický dokument]. DOI 10.1016/j.heliyon.2023.e23514 In: Heliyon. Amsterdam: Elsevier, 2024, Roč. 10, č. 1, art. no. e23514, s. [1-9] [online]. ISSN (online) 2405-8440. - SCO ; OA ; CCC ; WOS CC, s. [1 - 9]
2024 [01/20] / GBR - Mirzaei-Behbahani, Behnaz - Meratan, Ali Akbar - Moosakhani, Beitollah - Mohammad-Zaheri, Mahya - Mousavi-Jarrahi, Zahra - Nikfarjam, Nasser - Shahsavani, Mohammad Bagher - Saboury, Ali Akbar. Efficient inhibition of amyloid fibrillation and cytotoxicity of α-synuclein and human insulin using biosynthesized silver nanoparticles decorated by green tea polyphenols [elektronický dokument]. DOI 10.1038/s41598-024-54464-4 In: Scientific reports. Londýn: Springer Nature. Nature Publishing Group, 2024, Roč. 14, art. no. 3907, s. [1-17] [online]. ISSN (online) 2045-2322. - SCO ; OA ; CCC ; WOS CC, s. [1 - 17] |
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