| Basic data |
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| OCA1. Surname awarded to the assessed person | Legáth |
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| OCA2. Name awarded to the assessed person | Jaroslav |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, CSc. |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/2269 |
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| OCA5. Area of assessment | study programme animal hygiene and environment · III. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2025 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | |
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| OCA9. Hyperlink to the record in CRPA or CRAA | |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://doi.org/10.3390/ph18010027 |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | MARCINČÁKOVÁ, Dana - HUDÁKOVÁ, Nikola - MILEK, Michal - KOLESÁROVÁ, Mária - DZUGAN, Malgorzata - ČÍŽKOVÁ, Dáša - LEGÁTH, Jaroslav. Evaluation of the antioxidant properties and biological effects of a novel combined barberry root–propolis extract on HEK293T cells. In: Pharmaceuticals. 2025. ISSN 1424-8247, Vol. 18, no. 1 (2025), art. no. 27, p. [1-15]
(2024: 4.800 - IF, 4.900 - IF 5y, 1.019 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q) |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://www.mdpi.com/1424-8247/18/1/27 |
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| OCA14. Characteristics of the author's contribution | Coordination of scientific research |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | The health benefits of bee products and herbs are well known, and combining them appropriately can increase their biological effectiveness. This study investigated the biological properties of a combined extract of barberry root and propolis (PBE) compared to propolis extract (PE), barberry root extract (BE), and pure berberine (BN).
Antioxidant properties were evaluated using the DPPH and FRAP methods, and total phenolic content (TPC) was determined using the Folin–Ciocalteu method. The HPTLC method was used to quantify BE in the tested samples. Their effect on HEK293T cells was monitored in real time using the xCELLigence system, which recorded changes in proliferative activity (PA). Metabolic activity (MA) was evaluated using the MTS test, and cell migration was analyzed using the scratch test.
PE showed a higher TPC (198.67 mg/g) than BE (119.3 mg/g). PBE showed a comparable antioxidant effect to PE. In cell tests, PE, BE, and BN significantly reduced proliferative activity at higher concentrations (p < 0.0001), while PBE showed lower cytotoxicity and proved to be safer for the tested cells. The highest IC50 value was determined for PBE (130 µg/ml), indicating that this combination has reduced cytotoxicity. |
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| Other data |
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| OCA16. Annotation of the output in English | The health benefits of bee products and herbs are well known, and combining them appropriately can increase their biological effectiveness. This study investigated the biological properties of a combined extract of barberry root and propolis (PBE) compared to propolis extract (PE), barberry root extract (BE), and pure berberine (BN).
Antioxidant properties were evaluated using the DPPH and FRAP methods, and total phenolic content (TPC) was determined using the Folin–Ciocalteu method. The HPTLC method was used to quantify BE in the tested samples. Their effect on HEK293T cells was monitored in real time using the xCELLigence system, which recorded changes in proliferative activity (PA). Metabolic activity (MA) was evaluated using the MTS test, and cell migration was analyzed using the scratch test.
PE showed a higher TPC (198.67 mg/g) than BE (119.3 mg/g). PBE showed a comparable antioxidant effect to PE. In cell tests, PE, BE, and BN significantly reduced proliferative activity at higher concentrations (p < 0.0001), while PBE showed lower cytotoxicity and proved to be safer for the tested cells. The highest IC50 value was determined for PBE (130 µg/ml), indicating that this combination has reduced cytotoxicity. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | 1. 2025: [1] ZIENIUK, Bartłomiej - PAWEŁKOWICZ, Magdalena. Berberine as a Bioactive Alkaloid: Multi-Omics Perspectives on Its Role in Obesity Management. In Metabolites, 2025-07-01, 15, 7, pp. Dostupné na: https://doi.org/10.3390/metabo15070467. |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The health benefits of bee products and herbs are well known, and combining them appropriately can increase their biological effectiveness. This study investigated the biological properties of a combined extract of barberry root and propolis (PBE) compared to propolis extract (PE), barberry root extract (BE), and pure berberine (BN).
Antioxidant properties were evaluated using the DPPH and FRAP methods, and total phenolic content (TPC) was determined using the Folin–Ciocalteu method. The HPTLC method was used to quantify BE in the tested samples. Their effect on HEK293T cells was monitored in real time using the xCELLigence system, which recorded changes in proliferative activity (PA). Metabolic activity (MA) was evaluated using the MTS test, and cell migration was analyzed using the scratch test.
PE showed a higher TPC (198.67 mg/g) than BE (119.3 mg/g). PBE showed a comparable antioxidant effect to PE. In cell tests, PE, BE, and BN significantly reduced proliferative activity at higher concentrations (p < 0.0001), while PBE showed lower cytotoxicity and proved to be safer for the tested cells. The highest IC50 value was determined for PBE (130 µg/ml), indicating that this combination has reduced cytotoxicity. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The results published in this paper are useful in the educational process of training pharmacists. |
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