| Basic data |
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| OCA1. Surname awarded to the assessed person | Legáth |
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| OCA2. Name awarded to the assessed person | Jaroslav |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, CSc. |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/2269 |
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| OCA5. Area of assessment | study programme animal hygiene and environment · III. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2024 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | |
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| OCA9. Hyperlink to the record in CRPA or CRAA | |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://doi.org/10.3390/ani14111634 |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | BEKEŠOVÁ, Barbora - PETRILLA, Vladimír - POLLÁKOVÁ, Magdaléna - ANDREJČÁKOVÁ, Zuzana - VLČKOVÁ, Radoslava - DYBA, Barbara - SOPKOVÁ, Drahomíra - PETRILLOVÁ, Monika - PETROVOVÁ, Eva - LEGÁTH, Jaroslav. A comparative analysis of the cytotoxic and vascular activity effects of western diamondback rattlesnake (Crotalus atrox) and eastern diamondback rattlesnake (Crotalus adamanteus) venoms using a chick embryo model. In: Animals. 2024. ISSN 2076-2615, Vol. 14, no. 11 (2024), art. no. 1634, p. [1-14]
(2024: 2.700 - IF, 3.200 - IF 5y, 0.733 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q) |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://www.mdpi.com/2076-2615/14/11/1634 |
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| OCA14. Characteristics of the author's contribution | Coordination of the scientific team |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | Crotalus snake envenomation poses a serious challenge due to its diverse toxicological effects, including neurological, myotoxic, and cytotoxic symptoms, often leading to death. The aim of the study was to elucidate the physiological effects of exposure to Crotalus atrox and Crotalus adamanteus venoms and to assess toxicity using chicken embryo models. Currently, there is not a lot of research demonstrating the physiological effects of venom, including its potential impact on embryos, in accordance with the 3R rules. The applied research model consisted of the chick embryotoxicity screening test (CHEST) and the chick chorioallantoic membrane (CAM) test, which allowed for (i) a demonstration of the greater toxicity of C. adamanteus venom and (ii) an observation of the embryotoxic effect and vasoactive nature of the tested venom species. Additionally, (iii) morphological abnormalities (such as Siamese twins) emerged, and (iv) changes in the activity of acetylcholinesterase (AChE) were identified (resulting solely from its presence in the examined tissues due to the lack of this component in exogenously applied venom). These results provide crucial insights into the mechanisms of Crotalus venom toxicity and their potential biomedical applications. |
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| Other data |
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| OCA16. Annotation of the output in English | Crotalus snake envenomation poses a serious challenge due to its diverse toxicological effects, including neurological, myotoxic, and cytotoxic symptoms, often leading to death. The aim of the study was to elucidate the physiological effects of exposure to Crotalus atrox and Crotalus adamanteus venoms and to assess toxicity using chicken embryo models. Currently, there is not a lot of research demonstrating the physiological effects of venom, including its potential impact on embryos, in accordance with the 3R rules. The applied research model consisted of the chick embryotoxicity screening test (CHEST) and the chick chorioallantoic membrane (CAM) test, which allowed for (i) a demonstration of the greater toxicity of C. adamanteus venom and (ii) an observation of the embryotoxic effect and vasoactive nature of the tested venom species. Additionally, (iii) morphological abnormalities (such as Siamese twins) emerged, and (iv) changes in the activity of acetylcholinesterase (AChE) were identified (resulting solely from its presence in the examined tissues due to the lack of this component in exogenously applied venom). These results provide crucial insights into the mechanisms of Crotalus venom toxicity and their potential biomedical applications. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The study evaluated the embryotoxic effects of snake venoms from Crotalus adamanteus and Crotalus atrox species on the development of chicken embryos using the chick embryotoxicity screening test (CHEST) and the chorioallantoic membrane assay (HET-CAM). The results suggest that snake venoms may significantly affect embryonic development and the health of chicken embryos. C. adamanteus venom application revealed higher embryotoxicity and morphological abnormalities, such as Siamese twins, and exhibited greater toxicity. The administration of C. atrox venom caused more pronounced bleeding with a faster onset of action, which may relate to more hemotoxic components in C. atrox venom. Furthermore, the application of both venoms induced changes in the whole embryo, heart, and liver weights as well as massive hemorrhages in a dose-dependent manner with various intensities and a slight-to-moderate irritant potential. Specific concentrations of the venoms inhibited acetylcholinesterase activity in the liver and heart tissues of embryos. These results highlight the need for further studies and a better understanding of the toxicological effects of Crotalus snake venoms on different tissues and organisms. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | In addition to diploma theses, where proven methodologies can be used, the results published in the thesis can be presented in toxicology courses for pharmacists and veterinarians. |
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