KIRÁLY, Ján - HAJDUČKOVÁ, Vanda - GREGOVÁ, Gabriela - SZABÓOVÁ, Tatiana - PILIPČINEC, Emil. Resistant S. aureus isolates capable of producing biofilm from the milk of dairy cows with subclinical mastitis in Slovakia. In Agriculture. ISSN 2077-0472, 2024, vol. 14, no. 4, art. no. 571, p. [1-16].

Basic data
OCA1. Surname awarded to the assessed person	Gregová
OCA2. Name awarded to the assessed person	Gabriela
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6132
OCA5. Area of assessment	study programme animal hygiene and environment · III. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2024
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID: 1164390
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildUFPMA&sid=E2F93AEC495D93B320491E5E1A87&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.scopus.com/pages/publications/85191357114
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	KIRÁLY, Ján - HAJDUČKOVÁ, Vanda - GREGOVÁ, Gabriela - SZABÓOVÁ, Tatiana - PILIPČINEC, Emil. Resistant S. aureus isolates capable of producing biofilm from the milk of dairy cows with subclinical mastitis in Slovakia. In Agriculture. ISSN 2077-0472, 2024, vol. 14, no. 4, art. no. 571, p. [1-16].
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.mdpi.com/2077-0472/14/4/571
OCA14. Characteristics of the author's contribution	The author is the source of the main ideas and co-author of the output.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	Staphylococcus spp. is the most common cause of mastitis, with a significantly low cure rate. The work is focused on the assessment of antimicrobial resistance, identification of methicillin resistance genes, verification of biofilm formation and detection of genes associated with biofilm formation in S. aureus from milk samples from mastitic dairy cows.
Other data
OCA16. Annotation of the output in English	Staphylococcus spp. is the most common cause of mastitis, with a significantly low cure rate. Bacterial characteristics like adhesion and biofilm formation, as well as extracellular factors, can affect the pathogenesis of staphylococcal mastitis. The study’s objectives were to confirm S. aureus, assess their antibiotic resistance, identify methicillin resistance genes, verify biofilm formation, and detect biofilm-associated genes from bovine mastitis samples using multiplex PCR (mPCR). From 215 milk samples, six were confirmed as S. aureus. Most isolates were sensitive to all measured antibiotics. One isolate was identified as an inducible form of MLSB resistance (macrolides, lincosamides, and streptogramin B resistance), while the other two isolates were resistant to penicillins and carboxypenicillins. In S. aureus cultures used for methicillin resistance genotypic analysis by PCR, the mecA and mecC genes were not found. Biofilm formation phenotypes were determined in four strains. All strains of S. aureus carried icaABCD, agrA, srtA, fnbA, clfA, and clfB genes. Only in one isolate was the fnbB gene detected; the bap gene was not detected in any of the isolates. This emphasizes the importance of using appropriate treatment and continuous monitoring of S. aureus to prevent the spread of antibiotic-resistant strains in dairy cow farms.
OCA17. List of maximum 5 most significant citations corresponding to the output	1. Russo N., Fazio N.A., Licitra F., Gajewska J., Stamilla A., Salonia R., Chajęcka-Wierzchowska W., Randazzo C.L., Caggia C., Antoci F., Cascone G. Clonality, Virulence Genes, and Antimicrobial Resistance of Dairy Ruminants in Mastitic Milk-Associated Staphylococcus aureus in Sicily. (2025) Antibiotics, 14 (2), art. no. 188. DOI: 10.3390/antibiotics14020188 2. González-Machado C., Capita R., Alonso-Calleja C. Methicillin-Resistant Staphylococcus aureus (MRSA) in Dairy Products and Bulk-Tank Milk (BTM) (2024) Antibiotics, 13 (7), art. no. 588. DOI: 10.3390/antibiotics13070588 3. Son H.M., Duc H.M. Prevalence and Phage-Based Biocontrol of Methicillin-Resistant Staphylococcus aureus Isolated from Raw Milk of Cows with Subclinical Mastitis in Vietnam. (2024) Antibiotics, 13 (7), art. no. 638. DOI: 10.3390/antibiotics13070638 4. Alalaf A.S., Taha A.H., Sheet O.H. Genotypic characteristics and phylogenetic tree analysis of Staphylococcus aureus isolates from sheep subclinical mastitis milk. (2025) Open Veterinary Journal, 15 (1), pp. 289 – 299. DOI: 10.5455/OVJ.2025.v15.i1.27 5. Mencía-Ares O., Ramos-Calvo E., González-Fernández A., Aguarón-Turrientes Á., Pastor-Calonge A.I., Miguélez-Pérez R., Gutiérrez-Martín C.B., Martínez-Martínez S. Insights into the Virulence and Antimicrobial Resistance of Staphylococcus hyicus Isolates from Spanish Swine Farms. (2024) Antibiotics, 13 (9), art. no. 871. DOI: 10.3390/antibiotics13090871
OCA18. Characteristics of the output's impact on socio-economic practice	This publication offers new knowledge about the occurrence and spread of S. aureus in dairy farming. Excessive use of antibiotics in veterinary practice and failure to comply with good manufacturing practices in animal hygiene represent a significant factor in the emergence and spread of antibiotic-resistant microorganisms in animal farms. The occurrence of strains capable of forming biofilm in dairy herds points to the need to pay attention to compliance with good milking practices and to implement procedures for effective prevention, control and treatment of mammary gland infection.
OCA19. Characteristics of the output and related activities' impact on the educational process	The publication offers new knowledge about the occurrence and spread of S. aureus in dairy farming and contributes to expanding the knowledge of UVLF students in the field of the spread of antimicrobial resistance in animal husbandry. Antimicrobial resistance poses a serious global threat to humans, animals and the environment. In educational process of animal hygiene and welfare in UVLF, it is also necessary to focus on the use of appropriate methods of housing, sanitation and hygiene of farms to reduce the incidence of antimicrobial resistance of bacteria in farms.