| Basic data |
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| OCA1. Surname awarded to the assessed person | Trbolová |
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| OCA2. Name awarded to the assessed person | Alexandra |
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| OCA3. Degrees awarded to the assessed person | Prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6048 |
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| OCA5. Area of assessment | study programme veterinary nurse · I. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2026 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 1467703 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://app.crepc.sk/?fn=detailBiblioForm&sid=6CC5FEA37B00360243F638918D04 |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://arl4.library.sk/arl-uvlk/sk/vysledky/?field=AUPD&term=trbolov%C3%A1&search=H%C4%BEada%C5%A5&op=result&zf=&sort=&guide= |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | BALICKA, Agnieszka Alexandra - HUDÁKOVÁ, Nikola - LAPŠANSKÁ, Mária - RAŠIOVÁ, Adriána - TRBOLOVÁ, Alexandra - KONDRAKHOVA, Daria - KOMANICKÝ, Vladimír - VEČURKOVSKÁ, Ivana - BOBER, Peter - HORŇÁKOVÁ, Ľubica - VIKARTOVSKÁ, Zuzana - ČÍŽKOVÁ, Dáša. Impact of amniotic stem cell-conditioned medium on tear film integrity and MMP-9 levels in canine dry eye syndrome. In Experimental eye research. ISSN 0014-4835, 2026, vol. 262, no., art. no. 110736, p. [1-10]. (2024: 2.7 - IF, 3.3 - IF 5y, 153 - H-index, 0.965 - SJR, Q1 - SJR Best Q, Q2 - JCR Best Q, 1 - Nordic list). Dostupné na internete: . |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://pubmed.ncbi.nlm.nih.gov/41207525/ |
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| OCA14. Characteristics of the author's contribution | The author contributed to the design and implementation of an experimental study evaluating the effect of amniotic stem cell-conditioned medium in dogs with dry eye syndrome. Her contributions included clinical examinations, administration of treatment, assessment of tear film integrity, and determination of MMP-9 levels, as well as the processing and interpretation of the collected data. Her contributions also included the preparation of the manuscript, its peer review, and approval of the final version of the article. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | This article examines the potential use of a medium conditioned by amniotic stem cells in the form of eye drops for the treatment of qualitative keratoconjunctivitis sicca in dogs. The research process involved the design and implementation of an experimental clinical evaluation that monitored tear production, total protein concentration, MMP-9 levels, and tear film structure using the Schirmer test, biochemical analysis, and atomic force microscopy.
The results showed that in some of the treated dogs, there was an increase in tear production and protein concentration, as well as a decrease in MMP-9 levels, suggesting a possible anti-inflammatory and regenerative effect of the therapy. This study represents the first evaluation of this therapeutic strategy for dry eye in dogs and provides a foundation for further research, particularly regarding the optimization of dosage, duration of treatment, and the expansion of diagnostic and immunological parameters. |
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| Other data |
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| OCA16. Annotation of the output in English | This study evaluates the effect of eye drops containing a medium conditioned with amniotic stem cells in dogs with dry eye syndrome. The aim was to assess their impact on tear film production and stability, tear protein concentration, and levels of matrix metalloproteinase 9 (MMP-9), which is associated with inflammatory damage to the ocular surface. The results suggest an improvement in selected tear film parameters and a reduction in MMP-9, indicating the potential anti-inflammatory and regenerative effects of this therapy. The study’s findings support further investigation of amniotic stem cell-conditioned medium as a promising adjunctive treatment for keratoconjunctivitis sicca in dogs. |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | |
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| OCA18. Characteristics of the output's impact on socio-economic practice | This finding has the potential to expand treatment options for dry eye syndrome in dogs, a condition that reduces the animals’ quality of life and often requires long-term therapy. Eye drops containing a medium conditioned by amniotic stem cells may represent a promising complementary, cell-free, and regenerative treatment with potential anti-inflammatory effects. In half of the treated dogs, an increase in tear and protein production was observed, along with a decrease in MMP-9 levels, which supports further clinical validation of this method.
If efficacy is confirmed in larger-scale studies, the findings could lead to improved veterinary care, a reduction in the need for long-term symptomatic treatment, and the development of new veterinary ophthalmic products. The results also support collaboration between veterinary clinical practice, biomedical research, and the pharmaceutical sector. However, it should be noted that these are preliminary findings, and practical application requires further verification of safety, efficacy, optimal dosing, and affordability. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | This project expands educational content in veterinary medicine, particularly in the fields of veterinary ophthalmology, regenerative medicine, cell biology, and the diagnosis of dry eye syndrome in dogs. Based on this work, it is possible to demonstrate to students and professionals the principles of using a medium conditioned with amniotic stem cells, evaluating the tear film using the Schirmer test, determining MMP-9 levels, and utilizing atomic force microscopy to assess changes on the ocular surface.
The results can serve as a basis for practical instruction, seminars, master’s theses, and doctoral dissertations, as well as for further student research projects. At the same time, they promote the development of interdisciplinary thinking and bridge the gap between theoretical knowledge and clinical practice. Since this is an initial evaluation of this therapeutic method, students also learn about the importance of critically interpreting results, the limitations of pilot studies, and the need for further verification of the treatment’s efficacy and safety. |
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