ADM GREGOVÁ, Gabriela - KMEŤ, Vladimír - SZABÓOVÁ, Tatiana. New insight on antibiotic resistance and virulence of escherichia coli from municipal and animal wastewater. In Antibiotics. ISSN 2079-6382, 2021, vol. 10, no. 9, art. no. 1111, p. [1-11]. (2021: 5.222 - IF, 5.396 - IF 5y, 47 - H-index, 0.785 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q). Dostupné na internete: .

Basic data
OCA1. Surname awarded to the assessed person	Gregová
OCA2. Name awarded to the assessed person	Gabriela
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6132
OCA5. Area of assessment	study programme feed and foodstuff safety · I. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2021
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID: 423526
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioForm&sid=7115133E307B1B7E036054C7D5
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.scopus.com/pages/publications/85115341073?origin=resultslist
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	ADM GREGOVÁ, Gabriela - KMEŤ, Vladimír - SZABÓOVÁ, Tatiana. New insight on antibiotic resistance and virulence of escherichia coli from municipal and animal wastewater. In Antibiotics. ISSN 2079-6382, 2021, vol. 10, no. 9, art. no. 1111, p. [1-11]. (2021: 5.222 - IF, 5.396 - IF 5y, 47 - H-index, 0.785 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q). Dostupné na internete: .
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.mdpi.com/2079-6382/10/9/1111
OCA14. Characteristics of the author's contribution	The author is the source of the main ideas and co-author of the output.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	Processing of animal carcasses and other animal wastes in rendering plants is a significant source of antibiotic resistant microorganisms. The main goal of this study was to investigate the resistance to 18 antibacterial agents including β-lactams, fluoroquinolones, colistin and virulence factors (iss, tsh, cvaC, iutA, papC, kps and ibeA genes) in 88 Escherichia coli strains isolated from a rendering plant over 1 year period. ESBL (Extended-spectrum beta-lactamases) and plasmid-mediated Amp were screened by interpretative reading of MIC. ESBL phenotype was detected in 20.4% of samples and high level of resistance to fluoroquinolone was found in 27.2% of strains. Cephalosporinase CTX-M1, cephamycinase CMY-2, integrase 1 and transposon 3 genes were detected by PCR. Furthermore, there were found three CMY-2 producing E. coli with O25b-ST131, resistant to the high level of enrofloxacin and containing the gene encoding the ferric aerobactin receptor (iutA). One enrofloxacin resistant E. coli strain possessed iss, ibeA, kps and papC virulence genes also with CMY-2, integrase1 and Tn3. ST131 E. coli with CMY-2 has a zoonotic potential and presents a serious health risk to humans.
Other data
OCA16. Annotation of the output in English	Processing of animal carcasses and other animal wastes in rendering plants is a significant source of antibiotic resistant microorganisms. The main goal of this study was to investigate the resistance to 18 antibacterial agents including β-lactams, fluoroquinolones, colistin and virulence factors (iss, tsh, cvaC, iutA, papC, kps and ibeA genes) in 88 Escherichia coli strains isolated from a rendering plant over 1 year period. ESBL (Extended-spectrum beta-lactamases) and plasmid-mediated Amp were screened by interpretative reading of MIC. ESBL phenotype was detected in 20.4% of samples and high level of resistance to fluoroquinolone was found in 27.2% of strains. Cephalosporinase CTX-M1, cephamycinase CMY-2, integrase 1 and transposon 3 genes were detected by PCR. Furthermore, there were found three CMY-2 producing E. coli with O25b-ST131, resistant to the high level of enrofloxacin and containing the gene encoding the ferric aerobactin receptor (iutA). One enrofloxacin resistant E. coli strain possessed iss, ibeA, kps and papC virulence genes also with CMY-2, integrase1 and Tn3. ST131 E. coli with CMY-2 has a zoonotic potential and presents a serious health risk to humans.
OCA17. List of maximum 5 most significant citations corresponding to the output	KIMERA, Zuhura I. - MGAYA, Fauster X. - MISINZO, Gerald - MSHANA, Stephen E. - MOREMI, Nyambura - MATEE, Mecky I.N. Multidrug-resistant, including extended-spectrum beta lactamase-producing and quinolone-resistant, escherichia coli isolated from poultry and domestic pigs in dar es salaam, Tanzania. In Antibiotics, 2021-04-01, 10, 4, pp., Registrované v: Scopus Giovanni Dal Poggetto, M. Catauro, G. Crescente, C. Leonelli Efficient Addition of Waste Glass in MK-Based Geopolymers: Microstructure, Antibacterial and Cytotoxicity Investigation, Medicine Polymers, 2021. Registrované v: Scopus Urszula Zarzecka, Wioleta Chajęcka-Wierzchowska, Anna Zadernowska Occurrence of antibiotic resistance among Enterobacterales isolated from raw and ready-to-eat food – phenotypic and genotypic characteristics, Mar 2021Int J Environ Health Res, Registrované v: Scopus Wirth, Roland, et al. \"Functional resistomes in municipal wastewater treatment plants pose challenges to public health.\" Water Research (2025): 124663. Karahutová, Lívia, and Dobroslava Bujňáková. \"Occurrence of extended-spectrum β-lactamases and ertapenem-mono-resistance in Escherichia coli isolated from diarrheal calves.\" One Health (2025): 101138.
OCA18. Characteristics of the output's impact on socio-economic practice	Excessive use of antibiotics in veterinary practice and non-compliance with good manufacturing practice in animal hygiene is an important factor for the emergence and spread of antibiotic-resistant microorganisms. The presented publication is focused on the comparison of antibiotic resistance in E. coli bacteria in a rendering plant, which presents the picture of antibiotic resistance in farm animal. The processing of animal carcasses and other animal wastes in rendering plants is also an important source of the spread of antibiotic-resistant microorganisms into the environment.
OCA19. Characteristics of the output and related activities' impact on the educational process	Antimicrobial resistance poses a serious global threat to humans, animals and the environment. The publication offers an overview of resistance to individual ATBs in indicator bacteria from farms and in the treatment and farm hygiene. In educational process of animal hygiene and welfare in UVLF, it is also necessary to focus on the use of appropriate methods of housing, sanitation and hygiene of farms to reduce the incidence of antimicrobial resistance of bacteria in farms.