| Basic data |
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| OCA1. Surname awarded to the assessed person | Čurlík |
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| OCA2. Name awarded to the assessed person | Ján |
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| OCA3. Degrees awarded to the assessed person | Assoc. prof. , DVM, PhD |
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| OCA4. Hyperlink to the entry of the person in the Register of university staff | https://www.portalvs.sk/regzam/detail/6068 |
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| OCA5. Area of assessment | study programme cynology · I. degree |
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| OCA6. Category of the research/artistic/other output | scientific output |
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| OCA7. Year of publication of the research/artistic/other output | 2022 |
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| OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA) | 436815 |
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| OCA9. Hyperlink to the record in CRPA or CRAA | https://vsk.uvlf.sk/sk/my_pc/?pageno=2 |
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| Characteristics of the output that is not registered in CRPA or CRAA |
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| OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs | https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.801466/full |
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| OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs | HRUŠKOVICOVÁ, Jana - BHIDE, Katarína - PETROUŠKOVÁ, Patrícia - TKÁČOVÁ, Zuzana - MOCHNÁČOVÁ, Evelína - ČURLÍK, Ján - BHIDE, Mangesh Ramesh - KULKARNI, Amod. Engineering the single domain antibodies targeting receptor binding motifs within the domain III of West Nile Virus envelope glycoprotein. In Frontiers in Microbiology. ISSN 1664-302X, 2022, vol. 13, no., art. no. 801466, p. [1-15]. (2022: 5.2 - IF, 6.2 - IF 5y, 201 - H-index, 1.19 - SJR, Q1 - SJR Best Q, Q2 - JCR Best Q, 2 - Nordic list). Dostupné na internete: . |
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| OCA12. Type of the output (if the output is not registered in CRPA or CRAA) | article |
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| OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.) | https://pmc.ncbi.nlm.nih.gov/articles/PMC9012491/ |
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| OCA14. Characteristics of the author's contribution | The author contributed to the research conceptualization, data collection and analysis, interpretation of the results, and manuscript preparation, and collaborated on formulating its main objectives and scope. |
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| OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc. | The scientific output was created as part of the authors\' work on a neurotrophic flavivirus that causes mild febrile illness to encephalitis and acute flaccid paralysis with long-term or permanent neurological disorders. In the study, single-domain antibodies (sdAbs) against domain III (DIII) of the WNV envelope glycoprotein were developed to disrupt the interaction between DIII and human brain microvascular endothelial cells (hBMEC). |
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| Other data |
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| OCA16. Annotation of the output in English | Steps involving the isolation of peripheral blood mononuclear cells (PBMCs) from the blood of the immunized llama, amplification of VHH (variable domain of the heavy chain only antibodies, gene fragment encompassing framework 1-framework 4 of sdAbs), its cloning into phagemid pJB12, and subsequent transformation of E. coli XL-1 blue (New England Biolabs, Frankfurt, Germany) to generate the VHH-E. coli library were performed exactly as described in our earlier publication (Kulkarni et al., 2020). |
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| OCA17. List of maximum 5 most significant citations corresponding to the output | Verma, V., Sinha, N., & Raja, A. (2025, December). Nanoscale warriors against viral invaders: a comprehensive review of Nanobodies as potential antiviral therapeutics. In MAbs (Vol. 17, No. 1, p. 2486390). Taylor & Francis.
Minatel, V. M., Prudencio, C. R., Barraviera, B., & Ferreira Jr, R. S. (2024). Nanobodies: a promising approach to treatment of viral diseases. Frontiers in Immunology, 14, 1303353.
Jozefiaková, J., Kulkarni, A., Dhumal, D., Kevély, Á., Nováková, E., Koči, K., ... & Bhide, M. (2025). Nanobody conjugated with dendrimer nanoparticles effectively neutralizes tick-borne encephalitis virus. Scientific Reports, 15(1), 35482.
Mochnáčová, E., Bhide, K., Kucková, K., Jozefiaková, J., Maľarik, T., & Bhide, M. (2025). Antimicrobial cyclic peptides effectively inhibit multiple forms of Borrelia and cross the blood-brain barrier model. Scientific Reports, 15(1), 6147.
Fatima, M., An, T., Park, P. G., & Hong, K. J. (2025). Advancements and challenges in addressing zoonotic viral infections with epidemic and pandemic threats. Viruses, 17(3), 352. |
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| OCA18. Characteristics of the output's impact on socio-economic practice | The output represents the creation and methodological development of a library of VHH antibody fragments derived from an immunized llama, which constitutes an important tool for the further development of recombinant single-domain antibodies (sdAbs/nanobodies). The isolation of PBMCs, amplification of VHH genes, their cloning into the pJB12 phagemid, and subsequent transformation into E. coli enable the creation of a comprehensive VHH library that can serve as a source of candidate antibodies with high specificity and affinity toward selected target antigens.
From a socio-economic perspective, this output has the potential to contribute to the development of modern diagnostic procedures, particularly through the development of new tools for the detection and characterization of biologically relevant molecules. |
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| OCA19. Characteristics of the output and related activities' impact on the educational process | The scientific output has significant potential for improving the educational process in the field of molecular biology, virology, immunology and biomedical research. The acquired knowledge on the development and characterization of single-domain antibodies (sdAb) against West Nile virus (WNV) allows connecting the theoretical knowledge of students with practical procedures used in current biomedical research. The results of the study can be directly used in the educational process and topics focused on the structure and function of viral proteins, mechanisms of viral infection and neuropathogenesis, virus-host interactions, the production of recombinant proteins and antibodies, phage display, molecular cloning and others. The implementation of the results into the educational process will thus contribute to the modernization and updating of the teaching content. |
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