PETRILLA, Vladimír - POLLÁKOVÁ, Magdaléna - BEKEŠOVÁ, Barbora - ANDREJČÁKOVÁ, Zuzana - VLČKOVÁ, Radoslava - MARCINČÁKOVÁ, Dana - PETRILLOVÁ, Monika - PETROVOVÁ, Eva - SOPKOVÁ, Drahomíra - LEGÁTH, Jaroslav. A comprehensive study monitoring the venom composition and the effects of the venom of the rare ethiopian endemic snake species Bitis parviocula. In Toxins. ISSN 2072-6651, 2021, vol. 13, no. 5, art. no. 299, p. [1-22]. (2021: 5.075 - IF, 5.305 - IF 5y, 91 - H-index, 0.884 - SJR, Q1 - SJR Best Q, Q1

PETRILLA, Vladimír - POLLÁKOVÁ, Magdaléna - BEKEŠOVÁ, Barbora - ANDREJČÁKOVÁ, Zuzana - VLČKOVÁ, Radoslava - MARCINČÁKOVÁ, Dana - PETRILLOVÁ, Monika - PETROVOVÁ, Eva - SOPKOVÁ, Drahomíra - LEGÁTH, Jaroslav. A comprehensive study monitoring the venom composition and the effects of the venom of the rare ethiopian endemic snake species Bitis parviocula. In Toxins. ISSN 2072-6651, 2021, vol. 13, no. 5, art. no. 299, p. [1-22]. (2021: 5.075 - IF, 5.305 - IF 5y, 91 - H-index, 0.884 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q).

Basic data
OCA1. Surname awarded to the assessed person	Petrilla
OCA2. Name awarded to the assessed person	Vladimír
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/23878
OCA5. Area of assessment	study programme man-animal relationship and its use in canistherapy and hipotherapy · I. degree
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2021
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID: 253704
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildIFVVU&sid=39B3F1A9CB8F598F743F2CDD7C&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://arl4.library.sk/arl-uvlk/sk/detail-uvlk_un_cat-0037211-A-comprehensive-study-monitoring-the-venom-composition-and-the-effects-of-the-venom-of-the-rare-ethi/?disprec=1&iset=3
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	PETRILLA, Vladimír - POLLÁKOVÁ, Magdaléna - BEKEŠOVÁ, Barbora - ANDREJČÁKOVÁ, Zuzana - VLČKOVÁ, Radoslava - MARCINČÁKOVÁ, Dana - PETRILLOVÁ, Monika - PETROVOVÁ, Eva - SOPKOVÁ, Drahomíra - LEGÁTH, Jaroslav. A comprehensive study monitoring the venom composition and the effects of the venom of the rare ethiopian endemic snake species Bitis parviocula. In Toxins. ISSN 2072-6651, 2021, vol. 13, no. 5, art. no. 299, p. [1-22]. (2021: 5.075 - IF, 5.305 - IF 5y, 91 - H-index, 0.884 - SJR, Q1 - SJR Best Q, Q1 - JCR Best Q).
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.mdpi.com/2072-6651/13/5/299
OCA14. Characteristics of the author's contribution	Comprehensive preparation and management of the experiment, including the collection of snake venoms, the execution of toxicological and biochemical analyses on animal models in vivo and in vitro, the processing and statistical evaluation of obtained data, as well as the professional preparation of a scientific manuscript.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The Ethiopian endemic snake of the species Bitis parviocula, recognized for its colorful patterns, might be more interesting as we look deeper into the venom activity. We assayed the effects of venoms from the most widespread venomous African Bitis arietens and closely related species B. parviocula using The Hen’s Egg Test—Chorioallantoic membrane test and Chicken embryotoxicity screening test, acetylcholinesterase analysis, cytotoxicity assay performed on cell lines and protein analysis of selected venoms. Our results indicated that B. parviocula venom contains vasoactive compounds that have a direct effect on blood vessels. The AChE analysis showed significant ability inhibiting AChE activity in embryonic tissue. Cytotoxicity observed on carcinom cells indicates the possible presence of cytotoxic agents in B. parviocula venom. Based on similarities in vasoactive effects observed after administration of venoms onto a chicken chorioallantoic membrane, we suggest that venom from B. arietans and B. parviocula might share certain venom proteins responsible for haemotoxicity. The main active components of B. parviocula venom are unknown. Our results suggest that it might be worth performing proteomic analysis of B. parviocula venom as it might contain medically valuable compounds.
Other data
OCA16. Annotation of the output in English	The Ethiopian endemic snake of the species Bitis parviocula, recognized for its colorful patterns, might be more interesting as we look deeper into the venom activity. We assayed the effects of venoms from the most widespread venomous African Bitis arietens and closely related species B. parviocula using The Hen’s Egg Test—Chorioallantoic membrane test and Chicken embryotoxicity screening test, acetylcholinesterase analysis, cytotoxicity assay performed on cell lines and protein analysis of selected venoms. Our results indicated that B. parviocula venom contains vasoactive compounds that have a direct effect on blood vessels. The AChE analysis showed significant ability inhibiting AChE activity in embryonic tissue. Cytotoxicity observed on carcinom cells indicates the possible presence of cytotoxic agents in B. parviocula venom. Based on similarities in vasoactive effects observed after administration of venoms onto a chicken chorioallantoic membrane, we suggest that venom from B. arietans and B. parviocula might share certain venom proteins responsible for haemotoxicity. The main active components of B. parviocula venom are unknown. Our results suggest that it might be worth performing proteomic analysis of B. parviocula venom as it might contain medically valuable compounds.
OCA17. List of maximum 5 most significant citations corresponding to the output	KANE, Daniel - TAPLEY, Benjamin - CARTER, Kimberley C. - MICHAELS, Christopher J. Reproduction of the Ethiopian Mountain Adder, Bitis parviocula Böhme, 1976 (Reptilia: Viperidae), at ZSL London Zoo. In Herpetology Notes, 2022-01-14, 15, pp. 423-429., Registrované v: SCOPUS ALSHAMMARI, A.M. Screening of Phytochemicals Against Snake Venom Metalloproteinase: Molecular Docking and Simulation Based Computational Approaches. In Archives of Pharmacy Practice. ISSN 2320-5210, 2022, vol. 13, no. 3, p. 76-84., Registrované v: WoS PETROVIC, Marija - TANKOV, Stoyan - KIENING, Martin - CHAKRADHAR, Yakkala - RAFAEL, Duran - WALKER, Paul R. - BORCHARD, Gerrit - JORDAN, Olivier. How to outsmart the cold tumor microenvironment: Design of STING ligand nanoparticles for improved cancer immunotherapy. In OpenNano, 2023-07-01, 12, pp. ISSN 23529520. Dostupné na: https://doi.org/10.1016/j.onano.2023.100157., Registrované v: SCOPUS FEKI, A. - LAKHRAM, M. - BOUJHOUD, Z. - ESSAYAGH, S. - HILALI, S. - KALLEL, H. - AMARA, I. Chicken Embryo Model for in vivo Toxicological Study: Evaluation of Thiamethoxam Effect through HET-CAM and CHEST Methods. In Current Research in Life SciencesChapter. Publ. AkiNik Publications, 2024. Cgapter 2. P. 143. ISBN 978-93-6135-427-4.
OCA18. Characteristics of the output's impact on socio-economic practice	The expansion of knowledge on the biological activity of the venom of the endemic African snake Bitis parviocula may have significant implications for biomedical research and the development of new pharmacological agents. The identification of vasoactive, cytotoxic, and acetylcholinesterase-inhibiting effects indicates the presence of biologically active compounds with potential applications in drug development, particularly in the fields of cardiovascular diseases, neuropharmacology, and oncology research. The findings also support further proteomic investigation of the venom, which may lead to the discovery of new molecules with medical and biotechnological significance, thereby opening opportunities for innovation in the pharmaceutical industry and the transfer of knowledge into practice. In a broader economic context, this research may contribute to the development of biotechnology sectors and the strengthening of international scientific collaboration. Furthermore, the results expand the knowledge base on African venomous species, which is also relevant for toxicological safety, antivenom development, and the improvement of clinical management of snakebite envenomation, thereby indirectly contributing to the protection of public health.
OCA19. Characteristics of the output and related activities' impact on the educational process	The expansion and deepening of teaching in the fields of toxicology, physiology, and biomedical research through the integration of current experimental methods and real scientific data. Research findings on the venoms of snakes of the genus Bitis enable students to better understand the mechanisms of action of biologically active substances, including vasoactivity, cytotoxicity, and acetylcholinesterase inhibition, thereby enhancing their ability to link theoretical knowledge with experimental practice. Related activities, such as protein analysis, interpretation of results from embryonic and cell-based assays, and discussion of interspecies similarities in venoms, support the development of students’ critical thinking, scientific reasoning, and methodological skills. At the same time, they contribute to a better understanding of the importance of proteomics and modern bioanalytical techniques in venom research. The output thus contributes to improving the training of students in natural and medical sciences, supports their research competencies, and increases their readiness for work in biomedical and laboratory environments.