DANČOVÁ, Nikola - GREGOVÁ, Gabriela - SZABÓOVÁ, Tatiana. Assessment of bacterial contamination and antimicrobial resistance of Escherichia coli isolates from Slovak dairy farms. In Animals. ISSN 2076-2615, 2024, vol. 14, no. 21, art. no. 3095, p. [1-11].

Basic data
OCA1. Surname awarded to the assessed person	Gregová
OCA2. Name awarded to the assessed person	Gabriela
OCA3. Degrees awarded to the assessed person	Assoc. prof. , DVM, PhD
OCA4. Hyperlink to the entry of the person in the Register of university staff	https://www.portalvs.sk/regzam/detail/6132
OCA5. Area of assessment	habilitation and inauguration procedure – food hygiene and the environment
OCA6. Category of the research/artistic/other output	scientific output
OCA7. Year of publication of the research/artistic/other output	2024
OCA8. ID of the record in the Central Registry of Publication Activity (CRPA) or the Central Registry of Artistic Activity (CRAA)	ID: 1248813
OCA9. Hyperlink to the record in CRPA or CRAA	https://app.crepc.sk/?fn=detailBiblioFormChildQFQII&sid=DBBA30DD5E77A1386234EE194B45&seo=CREP%C4%8C-detail-%C4%8Cl%C3%A1nok
Characteristics of the output that is not registered in CRPA or CRAA
OCA10. Hyperlink to the record in another publicly accessible register, catalogue of research/ artistic/other outputs	https://www.scopus.com/pages/publications/85208631836
OCA11. Characteristics of the output in the format of the CRPA or the CRAA bibliographic record, if the output is not available in a publicly accessible register or catalogue of outputs	DANČOVÁ, Nikola - GREGOVÁ, Gabriela - SZABÓOVÁ, Tatiana. Assessment of bacterial contamination and antimicrobial resistance of Escherichia coli isolates from Slovak dairy farms. In Animals. ISSN 2076-2615, 2024, vol. 14, no. 21, art. no. 3095, p. [1-11].
OCA12. Type of the output (if the output is not registered in CRPA or CRAA)	article
OCA13. Hyperlink to the webpage where the output is available (full text, other documentation, etc.)	https://www.mdpi.com/2076-2615/14/21/3095
OCA14. Characteristics of the author's contribution	The author is the source of the main ideas and co-author of the output.
OCA15. Annotation of the output with contextual information concerning the description of creative process and the content of the research/artistic/other activity, etc.	The conditions in livestock housing are suitable for the survival of airborne microorganisms, mainly due to high temperatures, humidity, and the presence of organic material. The work is focused on the assessment of antimicrobial resistance in E.coli isolated from feces in cattle farms. We phenotypically and genotypically confirmed the resistance of E. coli isolated from cattle feces to the antimicrobial agents investigated. Our findings revealed the presence of multidrug-resistant E. coli strains.
Other data
OCA16. Annotation of the output in English	The conditions in livestock housing are suitable for the survival of airborne microorganisms, mainly due to high temperatures, humidity, and the presence of organic material. The total count of airborne bacteria concentrations in cattle farms ranged from 3.01 log10 CFU/mL to 6.90 log10 CFU/mL; for coliform bacteria, they were from 2.18 log10 CFU/mL to 3.34 log10 CFU/mL; and for molds, they ranged from 3.00 log10 CFU/mL to 4.57 log10 CFU/mL. Bacteria resistant to antimicrobial substances and resistance genes can be spread on animal farms. Antimicrobial resistance in ubiquitous Escherichia coli isolated from cattle feces was investigated. MIC testing was utilized to identify phenotypic resistance profiles, and the PCR method was employed to detect the presence of resistant genes. A higher percentage of resistance was found to amikacin (65%), tetracycline (61%), streptomycin (56%), ampicillin (55%), and nalidixic acid (45%). Multidrug resistance was determined in up to 64.3% of the isolates studied. The most widespread resistance genes were blaTEM (85.7%), sul2 (66.7%), tetB (52.38%), and sul1 (47.6%). We found that 4.8% of the E. coli isolates had the blaCMY gene. We found that, despite phenotypic resistance, E. coli isolates do not necessarily carry genes conferring resistance to that particular antimicrobial agent.
OCA17. List of maximum 5 most significant citations corresponding to the output	1. El-Sehrawy, A.A.M.A., Jasim, S.A., Jasim, H.F. et al. Comprehensive in silico genomics analysis of global trends and host-specific emergence of aminoglycoside resistance in Staphylococcus aureus: a One-Health perspective. BMC Microbiol 26, 766 (2026). https://doi.org/10.1186/s12866-026-05542-2 2. Bachevska, G.; Beev, G.; Dimov, D.; Stancheva, E.; Penev, T. A Comparison of Airborne Microbial Load on Four Housed Dairy Farms. Vet. Sci. 2026, 13, 357. https://doi.org/10.3390/vetsci13040357 3. Conny Riana Tjampakasari , Fithriyah Sjatha, Andi Yasmon, Agus Syahrurachman, Nadia Guntari Mutation analysis of dihydrofolate reductase (dfr) and dihydropteroate synthase (sul) genes in trimethoprim-sulfamethoxazole-resistant Escherichia coli from urinary tract infections. Iranian Journal of Microbiology, 2026, DOI https://doi.org/10.18502/ijm.v18i4.22197 4. László Ózsvári , Attila Dobos, Marietta Máté, Economic costs of veterinary drug and antibiotic use in commercial dairy cattle herds in Central European countries Front. Vet. Sci., 07 January 2026, Sec. Veterinary Epidemiology and Economics, Volume 12 – 2025, htps://doi.org/10.3389/fvets.2025.1714377 5. Lívia Karahutová, Dobroslava Bujňáková, Occurrence of extended- spectrum β-lactamases and ertapenem- mono- resistance in Escherichia coli isolated from diarrheal calves, One Health, Volume 21, 2025, 101138, ISSN 2352-7714, https://doi.org/10.1016/j.onehlt.2025.101138.
OCA18. Characteristics of the output's impact on socio-economic practice	The results of the study are practicaly applicable. It was studied the influence of microclimatic indicators on the incidence of E. coli in cattle farms. The conditions in livestock housing are suitable for the survival of airborne microorganisms, mainly due to high temperatures, humidity, and the presence of organic material. This publication offers an overview of resistance to individual ATBs in E. coli in livestock farms as well as recommendations for treatment and farm hygiene.
OCA19. Characteristics of the output and related activities' impact on the educational process	This publication offers new knowledge about the occurrence and spread of E. coli in dairy farming. Excessive use of antibiotics in veterinary practice and failure to comply with good manufacturing practices in animal hygiene represent a significant factor in the emergence and spread of antibiotic-resistant microorganisms in animal farms. In educational process of animal hygiene and welfare in UVLF, it is also necessary to focus on the use of appropriate methods of housing, sanitation and hygiene of farms to reduce the incidence of antimicrobial resistance of bacteria in farms.